FISH SCALE DERIVED COLLAGEN/GINSENOSIDE RB1 BIOCOMPOSITES: PREPARATION, CHARACTERIZATION AND THEIR HEMOSTATIC ABILITY

Authors

  • Chinh Thuy Nguyen Institute for Tropical Technology, VAST https://orcid.org/0000-0001-8016-3835
  • Tien Thuy Nguyen Hanoi University of Industry
  • Manh Quoc Vu Graduate University of Science and Technology
  • Hoang Thai Institute for Tropical Technology

DOI:

https://doi.org/10.15625/2525-2518/59/1/15423

Keywords:

Fish scale collagen, ginsenoside Rb1, characteristics, hemostatic ability, clotting time

Abstract

This paper reports the characteristics and hemostatic ability of the collagen/ginsenoside Rb1 composites with different ginsenoside Rb1 content, in which, collagen was extracted from fresh water fish scales and ginsenoside Rb1 was extracted from Panax notoginseng. Glucose and glutaraldehyde were used as crosslinking agents for collagen molecules. Infrared (IR) spectroscopy and Field emission scanning electron microscopy (FESEM) were applied to assess the functional groups, interactions and morphology of the collagen/ginsenoside Rb1 composites. Glutaraldehyde exhibits a positive effect on improvement the dispersion of ginsenoside Rb1 in collagen matrix as well as on the interactions between ginsenoside Rb1 and collagen. In addition, the biocompatibility of the collagen/ginsenoside Rb1 in simulated body fluid was evaluated by ultraviolet-visible spectroscopy (UV-Vis). The obtained result shows that ginsenoside Rb1 can release well from the composite containing 1 wt.% of ginsenoside Rb1. Besides, the hemostatic ability of the composites was also tested and discussed.

Downloads

Download data is not yet available.

Author Biography

Chinh Thuy Nguyen, Institute for Tropical Technology, VAST

Cán bộ nghiên cứu

References

Aras, O., Kazanci, M. - Production of collagen micro- and nanofibers for potential drug-carrier systems, Journal of Enzyme Inhibition and Medicinal Chemistry 30 (6) (2015) 1013–1016.

Ziemys, A., Yokoi, K., Kojic, M. - Capillary collagen as the physical transport barrier in drug delivery to tumor microenvironment, Tissue Barriers 3 (3) (2015) e1037418.

Yang, C., Wu, H., Wang, J. - Formulation and evaluation of controlled-release of steroidal saponins-loaded collagen microspheres, Materials Technology 34 (9) (2019) 534–539.

Petrisor, G., Ion, R. M., Brachais, C. H., Boni, G., Plasseraud, L., Couvercelle, J.P., Chambin, O. - In vitro release of local anaesthetic and anti-inflammatory drugs from crosslinked collagen based device, Journal of Macromolecular Science, Part A 49 (9) (2012) 699–705.

Qerimi, B., Baumann, P., Hüsing, J., Hanns-Peter, K., Schumacher, H. - Collagen hemostat significantly reduces time to hemostasis compared with cellulose: COBBANA, a single-center, randomized trial, The American Journal of Surgery 205 (6) (2013) 636-641.

Nakamura, N., Hashimoto, T., Sakai, H., Yasue, S., Nagayama, I., Sasaki, T., Basugi, N., Kim, K., Kitamura, K., Yoshimasu, M. - Clinical application of microfibrillar collagen hemostat (Avitene) in neurosurgical field, No Shinkei Geka 16 (2013) 933-938.

Tam, T. M., Binh, P. N. D. - Collecting collagen biofilms for treatment of burn from food processing industry source, Scientific Journal of Van Lang University 1 (2017) 85-92.

Jun, K. W., Kim, P. Y., Yong, Y. C., Timothy, T. Y., Nguan, S. T., Véronique, A., Cleo, C. - Fish scale-derived collagen patch promotes growth of blood and lymphatic vessels in vivo, Acta Biomater 63 (2017) 246-260.

Pathan, I. B., Munde, S. J., Shelke, S., Ambekar, W., Mallikarjuna Setty, C. - Curcumin loaded fish scale collagen-HPMC nanogel for wound healing application: Ex-vivo and In-vivo evaluation, International Journal of Polymeric Materials and Polymeric Biomaterials 68 (4) (2018) 165–174.

Chinh, N. T., Manh, V. Q., Thuy, P. T., Trung, V. Q., Quan, V. A., Giang, B. L., Hoang, T. - Novel pH-sensitive hydrogel beads based on carrageenan and fish scale collagen for allopurinol drug delivery, Journal of Polymers and the Environment 28 (6) (2020) 1795-1810.

Ramanathan, G., Singaravelu, S., Muthukumar, T., Thyagarajan, S., Rathore, H. S., Sivagnanam, U. T., Perumal, P. T. - Fabrication of Arothron stellatus skin collagen film incorporated with Coccinia grandis as a durable wound construct, International Journal of Polymeric Materials and Polymeric Biomaterials 66 (11) (2017) 558–568.

Xiaoyue, Y., Tang, C., Xiong, S., Yuan, Q., Gu, Z., Li, Z., Hu, Y. - Modification of collagen for biomedical applications: A review of physical and chemical methods, Current Organic Chemistry 20 (2016) 1797-1812.

Marissa, M. D., Lauren, S. B., Jenny, E. R., Alice, C., Abigail, C. - Flowable collagen-based hemostat and methods of use, US patent WO2012122044A2 (2012).

Lau, A. J., Toh, D. F., Chua, T. K., Pang, Y. K., Woo, S. O., Koh, H. L. - Antiplatelet and anticoagulant effects of Panax notoginseng: comparison of raw and steamed Panax notoginseng with Panax ginseng and Panax quinquefolium, Journal of Ethnopharmacology 125 (3) (2009) 380–386.

Ying, C. - Pharmacodynamical research and clinical application of Panax pseudo-ginseng saponins. Guangxi Medical Journal 20 (6) (1998) 1109–1112.

Byong-Kyu, S., Sung, W. K., Jeong, H. P. - Chemical diversity of ginseng saponins from Panax ginseng, Journal of Ginseng Research 39 (4) (2015) 287-298.

Qing, F. X., Liao, L. F., Dao, F. C. - Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats, Journal of Ethnopharmacology 84 (2–3) (2003) 187-192.

Yuan, Q., Jiang, Y. W., Ma, T. T., Fang, Q. H., Pan, L. - Attenuating effect of Ginsenoside Rb1 on LPS-induced lung injury in rats, Journal of Inflammation (London, England) 11 (1) (2014) 40.

Olde Damink, L. H. H., Dijkstra, P. J., Van Luyn, M. J. A. - Glutaraldehyde as a crosslinking agent for collagen-based biomaterials, Journal of Materials Science: Materials in Medicine 6 (1995) 460–472.

Downloads

Published

2021-01-15

Issue

Section

Materials