Quantitative analysis of Caffeine in the leaves and flowers of Camellia chrysantha by high-performance liquid chromatography with DAD detection

Pham Cao Bach, Nguyen Phi Hung, Cam Thi Inh, Pham Minh Quan, Tran Quoc Toan, Pham Thi Hong Minh, Pham Quoc Long, Nguyen Thi Hong Van
Author affiliations

Authors

  • Pham Cao Bach Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Nguyen Phi Hung Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Cam Thi Inh Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Pham Minh Quan Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Tran Quoc Toan Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Pham Thi Hong Minh Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Pham Quoc Long Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
  • Nguyen Thi Hong Van Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/58/3/14366

Keywords:

Caffeine, Camellia thrysantha, Golden Camellia, HPLC method

Abstract

Caffeine (1,3,7-trimethylxanthine) is the active alkaloid component which is a naturally occurring substance found in the leaves, seeds or fruits of over 63 plants species worldwide. Although caffeine intoxications are rare, they prove the toxic potential of this common constituent, resulting the tachycardia, atrial arrhythmias, convulsions or even coma. Caffeine toxic effect is increased when taken with other medicines, because it is metabolized through the cytochrome P450 system – primarily by the isoenzyme cYP1A2. This enzyme is also inhibited by antipsychotics and antiarrhythmic drugs, which could become toxic in presence of caffeine. This paper describes the methods for qualitative and quantitative determination of caffeine by HPLC. The results present optimal conditions for the rapid analysis of caffeine with high precision and accuracy which is suitable for its determination in the plant. The obtained results revealed that no caffeine is detected in the leaves and flowers of golden camellia (Camellia thrysantha). Thus, we may suggest that taking of golden camellia leaves and flowers as natural alternative to current decaffeinated tea could be avoid some unwanted side effects that caffeine produced beside the antioxidant content and health benefits of the plant material.

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Published

12-05-2020

How to Cite

[1]
P. C. Bach, “Quantitative analysis of Caffeine in the leaves and flowers of Camellia chrysantha by high-performance liquid chromatography with DAD detection”, Vietnam J. Sci. Technol., vol. 58, no. 3, pp. 267–273, May 2020.

Issue

Section

Natural Products

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