IMPEDIMETRIC IMMUNOSENSOR FOR ATRAZINE DETECTION BASED ON POLYPYRROLE NANOWIRES

Authors

  • Nguyen Van Anh School of Chemical Engineering, Hanoi University of Science and Technology
  • Nguyen Van Quan Institute of Materials Science, Vietnam Academy of Science and Technology
  • Nguyen Hai Binh
  • Nguyen Le Huy
  • Le Trong Huyen
  • Tran Dai Lam

DOI:

https://doi.org/10.15625/0866-708X/52/6/3595

Abstract

Animpedimetric immunosensor based on polypyrrole (PPy) nanowires was developedand applied for atrazine (ATZ) herbicide detection. In this work, the PPynanowires were synthesized by an electrochemical free-template method onto aninterdigitated platinum micro-electrode (IDµE). The immunosensors were preparedby immobilization of monoclonal anti-atrazine antibody (a-ATZ) onto a PPy surface using glutaraldehyde asa cross-linker. The electrodes were characterized using scanning electronmicroscopy (SEM), cyclic voltammetry (CV) and electrochemical impedancespectroscopy (EIS) techniques. The ATZ analysis is based on the immunoreactionof free ATZ and a-ATZ attached on top of the IDµE area (fingersand inter-digits space) by means of EIS. Thanks to immunoreagent specificity,these sensors exhibit high selectivity and sensitivity for ATZ detection with athreshold of 10 ng/mL

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Author Biography

Nguyen Van Quan, Institute of Materials Science, Vietnam Academy of Science and Technology

Phòng Vật liệu Nano Y-sinh, Viện Khoa học vật liệu

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Published

14-01-2015

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Articles