Isolation and identification of microorganisms causing water-based paint and wall paint destruction

Tran Thi Thanh Van; Bui Van Cuong; Cao Thi Hong; Nguyen Trung Huy; Vu Quoc Thai; Trinh Quang Dung; Nguyen Thi Xuyen; Vo Thi Kieu Anh; Nguyen Van `Trang; Tran Dai Lam

doi:10.15625/2525-2518/16672

Author affiliations

Authors

Tran Thi Thanh Van Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Bui Van Cuong Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Cao Thi Hong Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Nguyen Trung Huy Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Vu Quoc Thai Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Trinh Quang Dung Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Nguyen Thi Xuyen Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Vo Thi Kieu Anh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Nguyen Van `Trang Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam
Tran Dai Lam Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam

DOI:

https://doi.org/10.15625/2525-2518/16672

Keywords:

bacteria, biocorrosion, biodegradation, paint destruction, mold

Abstract

From samples of moldy wall paint and damaged water-based paint, we isolated and quantified the density of microorganisms present in the samples and determined the morphology of the isolates; thereby, identifying the genera of the bacteria and mold strains. The results showed that the density of the strains collected from the wall paint samples was much higher than that of the water-based paint samples (10⁶ CFU/mL and 10⁴ CFU/mL, respectively). In the wall paint samples, only mold colonies were observed, not bacterial colonies, while in the water-based paint samples both mold and bacterial colonies appeared, although mold colonies were still predominant. Based on the observation of colony formation and microscopic morphology of molds, we classified six mold strains into five genera: Aspergillus sp., Cladosporum sp., Acremoium sp., Chaetomium sp., and Fusarium sp. The frequency of strains belonging to the genus Aspergillus sp. accounted for the majority in both wall and water-based paint samples. Among the three bacterial strains isolated, we identified two bacterial strains as G (-) and one strain as G (+).

Downloads

Download data is not yet available.

References

Ravikumar H.R., Rao S.S., Karigar C.S., - Biodegradation of paints: a current status, Ind. J. Sci. and Technol. 5 (1) (2012) 1977–1987.DOI: 10.17485/ijst/2012/v5i1.33. DOI: https://doi.org/10.17485/ijst/2012/v5i1.33

Orehek J., Dogsa I., Tomšič M., Jamnik A., Kočar D., Stopar D., - Structural investigation of carboxymethyl cellulose biodeterioration by Bacillus subtilis subsp. subtilis NCIB 3610, Int. Biodeter.&Biodegrade. 77 (2013) DOI:10–17.10.1016/j.ibiod.2012.10.007. DOI: https://doi.org/10.1016/j.ibiod.2012.10.007

Obidi O.F., Aboaba O.O., Makanjuola M.S., Nwachukwu S.C.U., - Microbial evaluation and deterioration of paints and paint-products, J. Envir.Bio. 30 (5) (2009) 835–840.

Obidi O., Nwachukwu S., Aboaba O., - Investigation on the Biodegradative Potential of Pseudomonas aeruginosa on Water-based Paints, Researcher, 2 (1) (2010) 57–67.

Giacomucci L., Toja F., Sanmartín P., Toniolo L., Prieto B., Villa F., Cappitelli F., - Degradation of nitrocellulose–based paint by Desulfovibrio desulfuricans ATCC 1354, Biodegradation, 23 (2012) 705–716. DOI:10.1007/s10532-012-9546-9. DOI: https://doi.org/10.1007/s10532-012-9546-9

Ishfaq S., Ali N., Tauseef I., Khattak M.N.K., Shinwari Z.K., Ali M.I., - Analysis of paint degradation by fungal and bacterial species, Pakistan J. of Bot. 47 (2015) 753–760.

http://www.ftsl.itb.ac.id/kk/rekayasa_air_dan_limbah_cair/wp-ontent/uploads/2010/11/pe-ww7- dwipayana-15305020, 2016 (accessed 10 March 2016). http://www.ftsl.itb.ac.id/kk/rekayasa_air_dan_limbah_cair/wp-ontent/uploads/2010/11/pe-ww7- dwipayana-15305020, 2016 (accessed 10 March 2016).">

Wojturska J., - Enzymatic degradation of polyurethane coatings, Och. Prz. Kor. 52 (12) (2009) 595–600.

Halim A., El-Sayed M.M., Mahmoud W.M., Davis E.M., Coughlin R.W.,- Biodegradation of Polyurethane Coatings by Hydrocarbon-Degrading Bacteria, Inter. Biodeter.& Biodegrad. 37 (1996) 69–79. DOI: https://doi.org/10.1016/0964-8305(95)00091-7

Gautam R., Bassi A.S., Yanful E.K., Cullen E., - Biodegradation of automotive waste polyester polyurethane foam using Pseudomonas chlororaphis ATCC55729, Inter. Biodeter.& Biodegrad. 60 (2007) 245–249. DOI:10.1016/j.ibiod.2007.03.009. DOI: https://doi.org/10.1016/j.ibiod.2007.03.009

Howard G.T., - Microbial biodegradation of polyurethane. Recent Develop.in Poly. Rec., 2011.DOI:10.1016/S0964-8305(02)00051-3 DOI: https://doi.org/10.1016/S0964-8305(02)00051-3

Dang V. H. M. - Vietnam mold system. Science and Technics Publishing House, 2001.

Kirk P.M., Cannon P.F., Stalpers J.A., - Dictionary of the Fungi 10th edition. CABI, 2008.