ANTI-DIABETIC EFFECTS OF GLUCOMANNO-OLIGOSACCHARIDES VIA AMPK ACTIVATION IN C2C12 MYOTUBES
Keywords:glucomanno-oligosaccharides, glucose uptake, AMP-activated protein kinase, C2C12 myotube
AbstractAbstract-HCTN7. In this paper, antidiabetic activities of glucomanno-oligosaccharides (GMO) in vitro and in vivo were investigated. GMO significantly increased AMP-activated protein kinase (AMPK) phosphorylation in a concentration-independent manner. Treatment with 100μg/ml and 50μg/ml of GMO for 1 hour caused 1.47-fold and 1.81-fold phosphorylation of AMPK, respectively. Oral administration of GMO (6g/kg-1 of body weight day-1) lowered blood glucose levels (p < 0.05) at 120 min as compared to control group. These results suggested that GMO exhibited anti-diabetic effects via activation of AMPK and could be useful for diabetes prevention
K. Katsuraya, K. Okuyama, K. Hatanaka, R. Oshima, T. Sato, and K. Matsuzaki. - Constitution of konjac glucomannan: Chemical analysis and 13C NMR spectroscopy, Carbohydr. Polym. 53 (2) (2003) 183–189.
Mei Xu, Dong-Sheng Li, Bin Li, ChaoWang. - Comparative study on molecular weight of konjac glucomannan by gel permeation chromatography-laser light scattering-refractive index and laser light-scattering methods, J. Spectrosc. 1 (1) (2013) 2–6.
Bakht Ramin Shah, Bin Li, Ling Wang. - Health benefits of konjac glucomannan with special focus on diabetes, Bioact. Carbohydrates Diet. Fibre. 5 (2) (2015) 179–187.
Jianhua Liu, Qiuhong Xu, Jingjing Zhang. - Preparation, composition analysis and antioxidant activities of konjac oligo-glucomannan, Carbohydr. Polym. 130 (2015) 398–404.
L. John and A. Jeffery. - Beneficial effects of viscous dietary fiber from Konjac-mannan in subjects with the insulin resistance syndrome, Diabete Care 23 (1) (2000) 9–14.
Nobukazu Onishi, Sei ji Kawamoto. - A new immunomodulatory function of low-viscous konjac glucomannan with a small particle size: Its oral intake suppresses spontaneously occurring dermatitis in NC/Nga mice, Int. Arch. Allergy Immunol. 136 (3) (2005) 258–265.
S. L. Yeh, M. S. Lin, and H. L. Chen. - Partial hydrolysis enhances the inhibitory effects of konjac glucomannan from Amorphophallus konjac C. Koch on DNA damage induced by fecal water in Caco-2 cells, Food Chem. 119 (2) (2010) 614–618.
H. L. Chen, Y. H. Fan, M. E. Chen, and Y. Chan. - Unhydrolyzed and hydrolyzed konjac glucomannans modulated cecal and fecal microflora in Balb/c mice, Nutrition, 21 (10) (2005) 1059–1064.
M. Jiang, H. Li, J.-S. Shi, and Z.-H. Xu - Depolymerized konjac glucomannan: preparation and application in health care, J Zhejiang Univ-Sci B (Biomed Biotechnol). 19 (7) (2018) 505–514.
J. Liu, Y. Zhang, Y. Yin, F. Peng, P. Cai, and S. Yang. - Controlled acid hydrolysis and acetylation of glucomannans as drug carriers with designed pharmacokinetic behaviors, J. Control. Release. 152 (2011) e61–e62.
S. Kim, G. W. Go, and J. Y. Imm. - Promotion of Glucose Uptake in C2C12 Myotubes by Cereal Flavone Tricin and Its Underlying Molecular Mechanism, J. Agric. Food Chem., 65 (19) (2017) 3819–3826.
E. A. Richter and N. B. Ruderman. - AMPK and the biochemistry of exercise: Implications for human health and disease, Biochem. J. 418 (2) (2009) 261–275.
N. Musi, T. Hayashi, N. Fujii, M. F. Hirshman, L. A. Witters, and L. J. Goodyear. -AMP-activated protein kinase activity and glucose uptake in rat skeletal muscle, Am. J. Physiol. Metab. 280 (5) (2017) 677–684.
N. Musi and L. J. Goodyear. -AMP-activated Protein Kinase and Muscle glucose uptake Acta Physiol Scand. 178 (2003) 337–345.
Nu T. T., Nhi T. T. Y., and Thien D. T.. - Chemical structure of konjac glucomanno oligosaccharides prepared by, Vietnam J. Chem. 57 (2019) 291–295.
Nhi T. T. Y., Nu T. T., Thien D. T. - Response surface methodology for hydorolysis parameter optimization of konjac glucomannan using endo-1 , 4 β mannanase, Vietnam J. Chem., 57 (2019) 296–300.
B. T. Kurien and R. H. Scofield, - Western blotting, Methods. 38, (4) (2006) 283–293.
D. G. Hardie. - Role of AMP-activated protein kinase in the metabolic syndrome and in heart disease, FEBS Lett. 582 (1) (2008) 81–89.
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