Enzymatic hydrolysis of sea rough fish

Tran Thi Y Nhi, Ha Le Thi Thanh, Thien Do Truong
Author affiliations

Authors

  • Tran Thi Y Nhi Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Ha Le Thi Thanh Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam
  • Thien Do Truong Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Cau Giay District, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16667

Keywords:

alcalase/peptidase combined enzymes, sea rough fish

Abstract

In this stuty, sea rough fish were hydrolyzed with alcalase/peptidase combined enzymes (EA/EP). Response surface methodology (RSM) was employed to optimize the EA/EP, hydrolysis time, hydrolysis temperature, and enzyme to substrate ratio (E/S) to obtain a high hydrolysis degree. The optimum RSM operating conditions were EA/EP of 2.9, temperature 55.8oC, incubation time of 4.9 h, and substrate concentration at 0.45 %. Under these conditions, DH value of 56.2 % was obtained. Raw materials proximate composition, amino acid content of the hydrolysate was also evaluated. The results indicated that sea rough fish contains potentially valuable fraction with high protein content and were successfully converted into hydrolysates rich in essential amino acids for potential recommendations in the supplementation of animal and human diets.

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Published

17-04-2023

How to Cite

[1]
T. T. Y. Nhi, H. Le Thi Thanh, and T. Do Truong, “Enzymatic hydrolysis of sea rough fish”, Vietnam J. Sci. Technol., vol. 61, no. 2, pp. 199–208, Apr. 2023.

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