Degradation of deproteinized natural rubber by Gordonia sp. isolated from enrichment consortia

Nguyen Lan Huong; Nguyen Thi  Thanh; Nguyen Hoan Dung; Nguyen Thi Thu Trang; Pham Thi Quynh

doi:10.15625/2525-2518/58/1/14579

Author affiliations

Authors

Nguyen Lan Huong School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Thi Thanh School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Hoan Dung School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Thi Thu Trang School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Pham Thi Quynh School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/58/1/14579

Keywords:

isolation, deproteinized natural rubber, degradation, Gordonia sp.

Abstract

Biodegradation is a potential way of decomposing deproteinized natural rubber (DPNR). The enrichment consortia were demonstrated from a rubber processing factor waste. Nine DPNR-degrading bacteria were isolated from those consortia. The highest DPNR film weight loss in a mineral salt medium (MSM) was 43.92 ± 2.30 % after 30 days incubation using strain 5A1. The formation of aldehyde group during rubber degradation of 5A1 was determined using Schiff staining and Fourier Transform Infrared spectroscopy (FTIR) analysis. The 16S rRNA gene sequence, of 5A1 showed the highest identity with that of Gordonia soli CC-AB07. This is the first report to demonstrate a strong ability to degrade DPNR by Gordonia sp.

Downloads

Download data is not yet available.

References

Mooibroek H., and Cornish K.- Alternative sources of natural rubber. Appl. Microbiol. Biotechnol. 53 (2000) 355–365.

Huber M.A., and Terezhalmy G.T.- Adverse reactions to latex products: preventive and therapeutic strategies. J. Contemp Dent Pract., 7 (2006) 97-106.

Tsuchii, A., Takeda, K., and Tokiwa, Y.- Colonization and degradation of rubber pieces by Nocardia sp. Biodegradation, 7, (1996) 41-48.

Linos, A., Steinbüchel, A., Spröer, C., and Kroppenstedt, R. M.- Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from automobile tire, Int. J. Syst. Bacteriol., 49, (1999), 1785-1791.

Linos, A., Berekaa, M. M., Steinbüchel, A., Kim, K. K., Spröer, C., and Kroppenstedt, R. M.: Gordonia westfalica sp. nov., a novel rubber-degrading actinomycete, Int. J. Syst. Evol. Microbiol., 52, (2002), 1133-1139.

Hiessl S., Schuldes J., Thurmer A., Halbsguth T., Broker D., Angelov A., Liebl W., Daniel R., Steinbuchel A.,- Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2. Appl Environ Microbiol 78(8) (2012), 2874–2887.

Berekaa M. M., Lino A., Reichelt R., Keller U., and Steinbuchel A.- Effect of pretreatment of rubbber material on its biodegradability by various rubber degrading bacteria. FEMS Microbiol. Lett. 184 (2000), 199-206.

Braga S. P., Santos, A. P., Paganini T., Barbosa D Epamino G. W. C., Morais C., Martins L. F., Silva A. M., Setubal J. C., Vallim M. A., and Pascon R. C.- First report of cis-1,4-polyisoprene degradation by Gordonia paraffinivorans. Brazilian Journal of Microbiology, 2019, https://doi.org/10.1007/s42770-019-00143-w. https://doi.org/10.1007/s42770-019-00143-w.">

Linh D.V., Huong N.L., Tabata M., Imai S., Iijima S., Kasai D., Anh T.K., Fukuda M.- Characterization and functional expression of a rubber degradation gene of a Nocardia degrader from a rubber-processing factory.Journal of Bioscience and Bioengineering,123(4) (2017) 412-418.

Kawahara, S.; Klinklai, W.; Kuroda, H.; Isono, Y.- Removal of proteins from natural rubber with ure. Polym. Adv. Technol. 15(4), (2004) 181-184.

Nawong C., Umsakul K., and Sermwittayawong N.- Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples.Brazilian Journal of Microbiology, 49(3) (2018) 481-488.

Gallert C.- Degradation of Latex and of Natural Rubber by Streptomyces Strain La 7. System. Appl. Microbial. 23 (2000) 433-441.

Trang B.T., Linh D.V., Huong N.L., Anh T.K., Nghia P.T., Fukuda M.- Screening of natural rubber-degrading microorganisms from rubber processing factory waste in Vietnam. Internat. J. Waste Resources, 3 (2013) 9-12.

Hiessl S, Böse D, Oetermann S, Eggers J, Pietruszka J, Steinbüchel A.- Latex clearing protein an oxygenase cleaving poly(-1,4-isoprene) rubber at the cis double bond. Appl Environ Microbiol 80(2014) 5231-5240

Bosco F., Antonioli D., Casale A ,Gianotti V., Mollea C., Laus M., Malucelli G.- Biodegradation of unvulcanized natural rubber by microorganisms isolated from soil and rubber surface: A preliminary study. Bioremediation Journal, 22(1-2) (2018) 43-52.

Vidya T. V. and Growther L.- Biodegradation of rubber using actinobacteria isolated from rubber contaminated soil, Indian Journal of Environmental Protection, 37 (2017)1016-1025.

Andler R., Altenhoff A. L., Masing F., Steinbüchel A.- In vitro studies on the degradation of poly(cis-1,4-isoprene), Biotechnol Prog. 34(4) (2018) 890-899.

Linos A., Steinbüchel A. – Microbial degradation of natural and synthetic rubber by novel bacteria belonging to the genus Gordonia. Kautsch. Gummi Kunstst. 51 (1998), 496-499.

Linos A., Berekaa M.M., Reichelt R., Keller U., Schmitt J., Flemming H., Kroppenstedt R.M., and Steinbuchel A.- Biodegradation of cis-1,4-Polyisoprene Rubbers by Distinct Actinomycetes: Microbial Strategies and Detailed Surface Analysis. Applied and Environmental Microbiology, 66(4) (2000) 1639-1645.

Yikmis, M. and Steinbüchel, A.- Historical and recent achievements in the field of microbial degradation of natural and synthetic rubber, Appl. Environ. Microbiol., 78, (2012), 4543-4551.