Characterization of Tetragenococcus halophilus from Vietnamese fish sauce

Le Thanh Ha; Nguyen Nam Trinh; Le Thi My Chau; Nguyen Thi Minh Tu

doi:10.15625/2525-2518/57/5/13272

Author affiliations

Authors

Le Thanh Ha School of Biotechnology and Food Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Nam Trinh School of Biotechnology and Food Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Le Thi My Chau School of Chemistry, Biology and Enviroment, Vinh University, 182 Le Duan street, Vinh city, Nghe An province, Viet Nam
Nguyen Thi Minh Tu School of Biotechnology and Food Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/57/5/13272

Keywords:

fish sauce, Tetragenococcus halophilus, aminopeptidase activity, volatile compound

Abstract

The intracellular aminopeptidase activities and volatile compound profile of isolated halophilic lactic acid bacteria Tetragenococcus halophilus was investigated. Tetragenococcus halophilus CH2-4 and CH6-2 exhibited highest aminopeptidase activities 2.2 U/mL toward Glutamic-pNA. Meanwhile T. halophilus CH6-1 and V7-2 showed highest aminopeptidase activities 1.4 U/mL toward Leucine-pNA. The total 19 volatile compounds, including esters, alcohols, ketones, phenols, hydrocarbons and sulfur containing compounds were detected in fish broth inoculated with T. halophilus.T. halophilus CH6-2 produced most volatile compounds, 10/19 detected ones in this study.

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References

N. Udomsil, S. Rodtong, S. Tanasupawat, and J. Yongsawatdigul. Improvement of Fish Sauce Quality by Strain CMC5-3-1: A Novel Species of Staphylococcus sp. Journal of Food Science. 80 (2015) M2015-M2022.

J. Yongsawatdigul, S. Rodtong, and N. Raksakulthai. Acceleration of Thai Fish Sauce Fermentation Using Proteinases and Bacterial Starter Cultures. Journal of Food Science. 72 (2007) M382-M390.

T.V.A. Nguyễn, T.T.H. Bùi, Đ.T. Nguyễn, and T.H. Nguyễn. Tuyển chọn vi khuẩn lactic và Bacillus có khả năng chịu muối cao ứng dụng cho quá trình sinh hương trong sản xuất nước mắm. Hội nghị KHCN sinh học toàn quốc 2013. 2 (2013) 54-59.

N. Udomsil, S. Rodtong, Y.J. Choi, Y. Hua, and J. Yongsawatdigul. Use of Tetragenococcus halophilus as a Starter Culture for Flavor Improvement in Fish Sauce Fermentation. Journal of Agricultural and Food Chemistry. 59 (2011) 8401-8408.

M. Villar, A.P. de Ruiz Holgado, J.J. Sanchez, R.E. Trucco, and G. Oliver. Isolation and characterization of Pediococcus halophilus from salted anchovies (Engraulis anchoita). Applied and Environmental Microbiology. 49 (1985) 664-666.

K. Sakaguchi. Studies on the Activities of Bacteria in Soy Sauce Brewing. Bulletin of the Agricultural Chemical Society of Japan. 22 (1958) 345-362.

M. Satomi, B. Kimura, M. Mizoi, T. Sato, and T. Fujii. Tetragenococcus muriaticus sp. nov., a New Moderately Halophilic Lactic Acid Bacterium Isolated from Fermented Squid Liver Sauce. International Journal of Systematic and Evolutionary Microbiology. 47 (1997) 832-836.

S.H. Lee, J.Y. Jung, and C.O. Jeon. Bacterial community dynamics and metabolite changes in myeolchi-aekjeot, a Korean traditional fermented fish sauce, during fermentation. Int J Food Microbiol. 203 (2015) 15-22.

T.T. Nga, D.X. Tra, and L.T. Ha. Isolation of halophilic lactic bacteria Tetragenococcus halophilus from Vietnamese fish sauce. Vietnam Journal of Science and Technology. 55 (2017) 186-194.

A.A.A. Magboul and P.L.H. McSweeney. Purification and characterization of an aminopeptidase from Lactobacillus curvatus DPC2024. International Dairy Journal. 9 (1999) 107-116.

Y.-F. Yang, S.-R. Chen, H. Ni, and X.-Q. Ye. Analysis of volatile components in a Chinese fish sauce, Fuzhou Yulu, by gas chromatography-mass spectrometry. Journal of Zhejiang University Science B. 9 (2008) 977-981.

N. Udomsil, S. Rodtong, S. Tanasupawat, and J. Yongsawatdigul. Proteinase-producing halophilic lactic acid bacteria isolated from fish sauce fermentation and their ability to produce volatile compounds. International Journal of Food Microbiology. 141 (2010) 186-194.

M. Yvon and L. Rijnen. Cheese flavour formation by amino acid catabolism. 11 (2001) 185-201.

J.-N. Park, T. Watanabe, K.-I. Endoh, K. Watanabe, and H. Abe. Taste-active components in a Vietnamese fish sauce. Fisheries Science. 68 (2002) 913-920.

J. Jiang, Q. Zeng, and Z. Zhu. Analysis of Volatile Compounds in Traditional Chinese Fish Sauce (Yu Lu). Food Bioprocess Technol 4 (2011) 266-271.

M. Gürtler, M.G. Gänzle, G. Wolf, and W.P. Hammes. Physiological Diversity Among Strains of Tetragenococcus halophilus. Systematic and Applied Microbiology. 21 (1998) 107-112.