Factor influencing the survivability of Tetragenococcus halophilus CH6-2 in the spray drying process

Nguyen Trung Thanh, Vu Thi Kieu Oanh, Le Thanh Ha
Author affiliations

Authors

  • Nguyen Trung Thanh School of Biotechnology and Food Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
  • Vu Thi Kieu Oanh School of Biotechnology and Food Technology, Hanoi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
  • Le Thanh Ha School of Biotechnology and FoodTechnology, Hanoi University of Science and Technology,1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam https://orcid.org/0000-0003-4650-1639

DOI:

https://doi.org/10.15625/2525-2518/16310

Keywords:

cell survivability, growth conditions, heat stress, osmotic stress, protective agents

Abstract

In this study, several factors affecting Tetragenococcus halophilus CH6-2 cell survival rate in spray drying process including growth conditions, protective agents, and stress adaption were investigated. T. halophilus CH6-2, which was grown in different media, was mixed with different protective agents or at different ratios of cell concentration to protective agents or was subjected to stress adaption before spray drying. The survival rate after spray drying and after storage was determined. The results showed that skim milk had the strongest protective effect on T. halophilus CH6-2 cells during spray drying. De Man, Rogosa and Sharpe (MRS) was a better medium than our developed M7 optimal medium for culturing T. halophilus CH6-2 in respect of survivability after storage. Increasing the cell/protective agent ratio from 1/20 to 1/3 could increase the survivability after spray drying but not during storage. The heat stress and osmotic stress in this study did not improve the cell survivability after spray drying and storage.

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Published

28-02-2023

How to Cite

[1]
N. Trung Thanh, V. T. K. Oanh, and L. T. Ha, “Factor influencing the survivability of Tetragenococcus halophilus CH6-2 in the spray drying process”, Vietnam J. Sci. Technol., vol. 61, no. 1, pp. 47–56, Feb. 2023.

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Section

Natural Products

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