Improvement of SERS signal measured by portable Raman instrument using random sampling technique

Nguyen Van Tien; Nguyen  Trong Nghia; Nghiem Thi Ha Lien; Vu Duong; Do Quang Hoa; Duong Chi Dung; Phan Nguyen Nhue; Nguyen Minh Hue

doi:10.15625/2525-2518/16384

Author affiliations

Authors

Nguyen Van Tien Department of Physics, Graduate University of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Viet Nam https://orcid.org/0000-0002-2432-9527
Nguyen Trong Nghia Center for Quantum and Electronics, Institute of Physics, 18 Hoang Quoc Viet, Ha Noi, Viet Nam
Nghiem Thi Ha Lien Center for Quantum and Electronics, Institute of Physics, 18 Hoang Quoc Viet, Ha Noi, Viet Nam
Vu Duong Center for Quantum and Electronics, Institute of Physics, 18 Hoang Quoc Viet, Ha Noi, Viet Nam https://orcid.org/0000-0001-9813-723X
Do Quang Hoa Center for Quantum and Electronics, Institute of Physics, 18 Hoang Quoc Viet, Ha Noi, Viet Nam
Duong Chi Dung Department of Optical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet, Ha Noi, Viet Nam
Phan Nguyen Nhue Department of Optical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet, Ha Noi, Viet Nam
Nguyen Minh Hue Department of Physics, Le Quy Don Technical University, 236 Hoang Quoc Viet, Ha Noi, Viet Nam https://orcid.org/0000-0002-6059-6964

DOI:

https://doi.org/10.15625/2525-2518/16384

Keywords:

β-galactosidase, hemolytic activity, Lactobacillus casei, probiotic

Abstract

In recent years, portable Raman spectrometers and commercialized surface-enhanced Raman scattering (SERS) substrates have become increasingly popular. They have turned out to be great tools for both substance detection, identification, and analysis on-site. This work addresses the technique to collect proper Raman spectra using SERS substrates and portable Raman spectrometers. We propose a random sampling technique that gives representative and high-quality spectra with high intensity and good resolution. This technique was tested on a home-built portable Raman spectrometer and SERS substrates based on metal film over nano-sphere (MFON) structure. Experimental results showed that peaks of Raman spectrum collected using random sampling technique are significantly narrower than those of spectra measured in conventional one and prevent samples and SERS substrates from photoinduced degradation. Potentially, this method can promote quantitative SERS and chemical trace analysis using portable Raman spectrometers.

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