Primary study on the probiotic properties of Lactobacillus casei LC 304.08 following international standard

Nguyen Ngoc Hong; Van Huong Giang; Nguyen Thi Phuong; Dao Ngoc Ha; Nguyen Duy Ha; Le Huy Hoang; Nguyen Quynh Uyen; Hoang Van Vinh

doi:10.15625/2525-2518/16284

Author affiliations

Authors

Nguyen Ngoc Hong VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Van Huong Giang VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Nguyen Thi Phuong VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Dao Ngoc Ha VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Nguyen Duy Ha Military Institute of Preventive Medicine, 21 Trung Liet, Dong Da, Hanoi, Viet Nam
Le Huy Hoang Institute of New Technology, Academy of Military Science and Technology, 17 Hoang Sam, Cau Giay, Hanoi, Viet Nam
Nguyen Quynh Uyen VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Hoang Van Vinh VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16284

Keywords:

β-galactosidase, hemolytic activity, , Lactobacillus casei

Abstract

Abstract Products containing beneficial bacteria for humans have been widely used recently. The bacteria in these products generally belong to the genus Lactobacillus. Among the species of this genus, Lactobacillus casei attracts a lot of interest due to their valuable properties. With these properties, many L.casei strains can be used as probiotics. Based on the recommendation of FAO/WHO for probiotic bacteria, in our study strain LC 304.08 was identified based on morphology and biochemical properties as well as nucleotide sequence of 16S rDNA. The strain was then evaluated for its properties in vitro such as the ability to survive at low pH and in the presence of bile salt, the ability to produce antibacterial substances and enzyme as well as its safety regarding hemolytic and antibiotic resistance. The results showed that the strain was identified as Lactobacillus casei. It survived well at low pH and in the presence of bile salt, and it produced β-galactosidase with the activity of 81 ± 1.1 (U/mL). In addition, the strain was safe regarding its hemolytic activity and antibiotic resistance.

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