Primary study on the probiotic properties of Lactobacillus casei LC 304.08 following international standard

Nguyen Ngoc Hong; Van Huong Giang; Nguyen Thi Phuong; Dao Ngoc Ha; Nguyen Duy Ha; Le Huy Hoang; Nguyen Quynh Uyen; Hoang Van Vinh

doi:10.15625/2525-2518/16284

Author affiliations

Authors

Nguyen Ngoc Hong VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Van Huong Giang VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Nguyen Thi Phuong VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Dao Ngoc Ha VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Nguyen Duy Ha Military Institute of Preventive Medicine, 21 Trung Liet, Dong Da, Hanoi, Viet Nam
Le Huy Hoang Institute of New Technology, Academy of Military Science and Technology, 17 Hoang Sam, Cau Giay, Hanoi, Viet Nam
Nguyen Quynh Uyen VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
Hoang Van Vinh VNU, Institute of Microbiology and Biotechnology, Vietnam National University Hanoi, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16284

Keywords:

β-galactosidase, hemolytic activity, , Lactobacillus casei

Abstract

Abstract Products containing beneficial bacteria for humans have been widely used recently. The bacteria in these products generally belong to the genus Lactobacillus. Among the species of this genus, Lactobacillus casei attracts a lot of interest due to their valuable properties. With these properties, many L.casei strains can be used as probiotics. Based on the recommendation of FAO/WHO for probiotic bacteria, in our study strain LC 304.08 was identified based on morphology and biochemical properties as well as nucleotide sequence of 16S rDNA. The strain was then evaluated for its properties in vitro such as the ability to survive at low pH and in the presence of bile salt, the ability to produce antibacterial substances and enzyme as well as its safety regarding hemolytic and antibiotic resistance. The results showed that the strain was identified as Lactobacillus casei. It survived well at low pH and in the presence of bile salt, and it produced β-galactosidase with the activity of 81 ± 1.1 (U/mL). In addition, the strain was safe regarding its hemolytic activity and antibiotic resistance.

Downloads

Download data is not yet available.

References

FAO/WHO - Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria, Food and Agriculture Organization of the United Nations and World Health Organization, Joint FAO/WHO Expert Consultation Group: Cordoba, Argentina, 2001.

Anandharaj M., Rizwana P. R. and Swain R. M. - Production of high quality probiotics by fermentation, in: Gupta K. V., Treichel H., Shapaval V., Oliveira A. L. and Tuohy G. M. (Eds.), Microbial Functional Foods and Nutraceuticals, John Wiley & Sons Ltd., 2018, pp. 235–266. DOI: https://doi.org/10.1002/9781119048961.ch10

Đo T. B. T. and Nguyen T. D. H. - Determination of salt tolerance and some potential probiotic properties of lactic acid bacteria strains of species Lactobacillus fermentum isolated from gut of scad fish (Decapterus lajang), Vietnam Journal of Vietnam Agricultural Science and Technology 2 (2) (2018) 799–805, (in Vietnamese).

Ho L. Q. C., Ho T. T., Nguyen T. K. Q., Tran T. H. - Study on aggregation ability and antibacterial characteristics in in vitro condition of nine microorganism strains with potentials as probiotics, Hue University Journal of Science 57 (2010) 5–13.

Saitou N. and Nei M. - The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol. 4 (1987) 406. https://doi.org/10.1093/oxfordjournals.molbev.a040454. DOI: https://doi.org/10.1093/oxfordjournals.molbev.a040454 https://doi.org/10.1093/oxfordjournals.molbev.a040454.">

Felsen S. J. - Confidence limits on phylogenies: an approach using the bootstrap, Evolution 39 (1985) 783. http://dx.doi.org/10.2307/2408678 DOI: https://doi.org/10.2307/2408678 http://dx.doi.org/10.2307/2408678">

Clinical and Laboratory Standard Institute Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, 10th edition, Clinical and Laboratory Standard Institute, Wayne, Pa (2009), USA.

Fijan S. - Antimicrobial effect of probiotics against common pathogens, in: Dr. Venketeshwer Rao (Ed.), Probiotics and prebiotics in human nutrition and health, IntechOpen, 2016, pp. 191-221. DOI: https://doi.org/10.5772/63141

Vinderola C. G. and Reinheimer J. A. - Lactic acid starter and probiotic bacteria, a comparative “in vitro” study of probiotic characteristics and biological barrier resistance, J. Food Res. Int. 36 (2003) 895-904. https://doi.org/10.1016/S0963-9969(03)00098-X DOI: https://doi.org/10.1016/S0963-9969(03)00098-X https://doi.org/10.1016/S0963-9969(03)00098-X">

Noriega L., Gueimonde M., Sa´nchez B., Margolles A. and de los Reyes-Gavila´n G. C. - Effect of the adaptation to high bile salts concentrations on glycosidic activity, survival at low PH and cross-resistance to bile salts in Bifidobacterium, Int. J. Food Microbiol. 94 (2004) 79–86. https://doi.org/10.1016/j.ijfoodmicro.2004.01.003 DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.01.003 https://doi.org/10.1016/j.ijfoodmicro.2004.01.003">

Dunne C., O’Mahony L., Murphy L., Thornton G., Morrissey D., O’Halloran S., Feeney M., Flynn S., Fitzgerald G., Daly C., Kiely B., O’Sullivan C. G., Shanahan F. and Collins K. J. - In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings, Am J Clin Nutr 73 (suppl) (2001) 386S–92S. https://doi.org/10.1093/ajcn/73.2.386s. DOI: https://doi.org/10.1093/ajcn/73.2.386s https://doi.org/10.1093/ajcn/73.2.386s.">

Pisano B. M., Viale S., Conti S., Fadda E. M., Deplano M., Melis P. M., Deiana M. and Cosentino S. - Preliminary evaluation of probiotic properties of Lactobacillus strains isolated from Sardinian Dairy Products, Biomed Res Int (2014) 9. http://dx.doi.org/10.1155/2014/286390. DOI: https://doi.org/10.1155/2014/286390 http://dx.doi.org/10.1155/2014/286390.">

Sutula J., Coulthwaite L. and Verran J. - Culture media for differential isolation of Lactobacillus casei Shirota from oral samples, J. Microbiol. Methods 90 (2012) 65–71. http://dx.doi.org/10.1016/j.mimet.2012.03.015. DOI: https://doi.org/10.1016/j.mimet.2012.03.015 http://dx.doi.org/10.1016/j.mimet.2012.03.015.">

Widodo H., Taufi T. T., Aryati E., Kurniawati A. and Asmara W. - Human origin Lactobacillus casei isolated from Indonesian infants demonstrating potential characteristics as probiotics in vitro, Indones. J. of Biotechnol. 17 (1) (2012) 79–89. https://doi.org/10.22146/ijbiotech.7852 https://doi.org/10.22146/ijbiotech.7852">

Sharma K., Sharma N. and Sharma R. - Identification and Evaluation of In vitro Probiotic Attributes of Novel and Potential Strains of Lactic Acid Bacteria Isolated from Traditional Dairy Products of North-West Himalayas, J. Clin. Microbiol. Biochem. Technol. 2 (1) (2016) 018-025. DOI: https://doi.org/10.17352/jcmbt.000011

Delcour J., Ferain T., Deghorain M., Palumbo E. and Hols P. - The biosynthesis and functionality of the cell-wall of lactic acid bacteria, Antonie Van Leeuwenhoek 76 (1999) 159–184. https://doi.org/10.1023/A:1002089722581 DOI: https://doi.org/10.1007/978-94-017-2027-4_7 https://doi.org/10.1023/A:1002089722581">

Sharma P., Tomar S. K., Goswami P., Sangwan V. and Singh R. - Antibiotic resistance among commercially available probiotics, Food Res. Int. 57 (2014) 176–195. https://doi.org/10.1016/j.foodres.2014.01.025. DOI: https://doi.org/10.1016/j.foodres.2014.01.025 https://doi.org/10.1016/j.foodres.2014.01.025.">

Gueimonde M., Sánchez B., de Los Reyes-Gavilán C. G. and Margolles A. - Antibiotic resistance in probiotic bacteria, Front. Microbiol. 4 (2013) 202. https://doi.org/10.3389/fmicb.2013.00202. DOI: https://doi.org/10.3389/fmicb.2013.00202 https://doi.org/10.3389/fmicb.2013.00202.">

Campedelli I., Mathur H., Salvetti E., Clarke S., Rea M. C., Torriani S., Ross R. P., Hill C. and O’Toole P. W. - Genus-wide assessment of antibiotic resistance in Lactobacillus spp, Appl. Environ. Microbiol. 85 (2019) e01738-18. https://doi.org/1128/AEM.01738-18. DOI: https://doi.org/10.1128/AEM.01738-18 https://doi.org/1128/AEM.01738-18.">

Kirtzalidou E., Pramateftaki P., Kotsou M. and Kyriacou A. - Screening for lactobacilli with probiotic properties in the infant gut microbiota, Anaerobe 17 (6) (2011) 440–443. https://doi. org/10.1016/j.anaerobe.2011.05.007. DOI: https://doi.org/10.1016/j.anaerobe.2011.05.007