NGHIÊN CỨU CHIẾT PHLOROTANNIN VỚI HOẠT TÍNH CHỐNG OXY HÓA TỪ RONG NÂU Sargassum mcclurei BẰNG PHƯƠNG PHÁP NGÂM DẦM CÓ HỖ TRỢ VI SÓNG

Abstract

The extraction condition for phlorotannin with antioxidant activity from brown algae Sargassum mcclurei was focused to research in the paper. Microwave-assisted maceration method with the 100% ethanol 96⁰ extraction solvent was used to research. Phlorotannin content with total antioxidant activity and reducing power activity were evaluated. Some factors of extraction step ( microwave power: 100 – 800W; the ratio of solvent-material from 10:1 to 50:1 (w/w); time: 1 – 5 minutes; pH 5 – 9 and times) were researched. The result showed that the fit extraction condition was as follow: the microwave power of 100W, the solvent-material ratio of 30:1 (v/w), the time of 2 minutes, pH of 7 and the 3 times). At this fit extraction condition, phlorotannin content with total antioxidant activity and reducing power activity were corresponded with 3,88 ± 0,033 mg phloroglucinol/g DW, 15,096 ± 0,037 mg acid ascorbi/g DW and 5,365 ± 0,028 mg FeSO₄/ g DW. ANOVA analysis showed that the object functions were correlated each other follow the non-linear function.