Analyzing movement behavior of zebrafish  in different sized confined areas

Quach Kha Quang; Nguyen Quoc Anh

doi:10.15625/2525-2518/16476

Author affiliations

Authors

Quach Kha Quang International Affairs Office, No.783, Pham Huu Lau street, Ward 6, Cao Lanh city, Dong Thap, Viet Nam
Nguyen Quoc Anh International Affairs Office, International Affairs Office, No.783, Pham Huu Lau street, Ward 6, Cao Lanh city, Dong Thap, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16476

Keywords:

Confined areas, Monitoring, Movement behavior, Zebrafish (Danio rerio)

Abstract

Movement behavior of zebrafish (Danio rerio) was analysed according to different sizes of observation arena (four sizes:and ). The observation arena was separated into corner, boundary and central areas based on experimental data. The results showed that the shapes of the corner, boundary and central areas were accordingly different in different sizes of the arena. Individuals stayed in the boundary zone for a substantial proportion (approximately 55-58 %) of the observation period while in the corner zone they stayed for the shortest time period (approximately 12-14 %). Movement parameters (speed, acceleration, etc.) of each individual varied in different areas on different sizes and speed was highest in the size of while acceleration was highest in the size of . These reveal that the movement behaviors of zebrafish were affected by different sizes of arenas, at larger sizes they move more actively compared to smaller sizes. However, Transition probability matrices (TPMs) of moving between different areas in the observation were stable. These findings imply that there is a stereotypic inner state that maintains basic behaviors in animals. Information from this work would provide backgrounds of real-life process mechanism and would be useful for monitoring in response to environmental changes in practical aspect and be applicable to a wide range of fields including pharmacology, neurology, and genetics.

Downloads

Download data is not yet available.

References

Brodin T., Nordling J., Lagesson A., Klaminder J., Hellström G., Christensen B., and Fick J. - Environmental relevant levels of a benzodiazepine (oxazepam) alters

important behavioral traits in a common planktivorous fish, (Rutilus rutilus), Journal of Toxicology and Environmental Health, Part A, 80 (16-18) (2017) 963-970. https://doi.org/10.1080/15287394.2017.1352214 DOI: https://doi.org/10.1080/15287394.2017.1352214

Saaristo M., Brodin T., Balshine S., Bertram M. G., Brooks B. W., Ehlman S. M., McCallum E. S., Sih A., Sundin J., Wong B. B., and Arnold K. E. - Direct and indirect effects of chemical contaminants on the behaviour, ecology and evolution of wildlife, Proceedings of the Royal Society B 285 (1885) (2018) 20181297. https://doi.org/10.1098/rspb.2018.1297 DOI: https://doi.org/10.1098/rspb.2018.1297

Araujo-Silva H., Pinheiro-da-Silva J., Silva P. F., and Luchiari A. C. - Individual differences in response to alcohol exposure in zebrafish (Danio rerio), PLoS One 13 (6) (2018) e0198856. https://doi.org/10.1371/journal.pone.0198856. DOI: https://doi.org/10.1371/journal.pone.0198856

Severo E. S., Marins A. T., Cerezer C., Costa D., Nunes M., Prestes Zanella R., and Loro V. L. - Ecological risk of pesticide contamination in a Brazilian river located near a rural area: a study of biomarkers using zebrafish embryos, Ecotoxicology and environmental safety 190 (2020) 110071. https://doi.org/10.1016/j.ecoenv.2019.110071 DOI: https://doi.org/10.1016/j.ecoenv.2019.110071

Eilam D. - Open-field behavior withstands drastic changes in area size, Behavioural Brain Research, 142 (1-2) (2003) 53-62. https://doi.org/10.1016/S0166-4328(02)00382-0. DOI: https://doi.org/10.1016/S0166-4328(02)00382-0

Stewart A. M., Gaikwad S., Kyzar E., and Kalueff A. V. - Understanding spatio-temporal strategies of adult zebrafish exploration in the open field test, Brain research 1451 (2012) 44-52. https://doi.org/10.1016/j.brainres.2012.02.064 DOI: https://doi.org/10.1016/j.brainres.2012.02.064

Yaski O., Portugali J., and Eilam D. - Arena geometry and path shape: When rats travel in

straight or in circuitous paths?, Behavioural brain research 225 (2) (2011) 449-454. https://doi.org/10.1016/j.bbr.2011.07.055 DOI: https://doi.org/10.1016/j.bbr.2011.07.055

Jeanson R., Blanco S., Fournier R., Deneubourg J. L., Fourcassié V., and Theraulaz G. - A model of animal movements in a bounded space, Journal of Theoretical Biology 225 (2003a) 443-451. https://doi.org/10.1016/S0022-5193(03)00277-7. DOI: https://doi.org/10.1016/S0022-5193(03)00277-7

Braun C. D., Gaube P., Afonso P., Fontes J., Skomal G. B., and Thorrold S. R. - Assimilating electronic tagging, oceanographic modelling, and fisheries data to estimate movements and connectivity of swordfish in the North Atlantic, ICES Journal of Marine Science, 76 (7) (2019), 2305-2317. https://doi.org/10.1093/icesjms/fsz106 DOI: https://doi.org/10.1093/icesjms/fsz106

Liu Y., Chon T. S., and Lee S. H. - Analysis of behavioral changes of zebrafish (Danio rerio) in response to formaldehyde using Self-organizing map and a hidden Markov model, Ecological Modelling, 222 (14) (2011) 2191-2201.

https://doi.org/10.1016/j.ecolmodel.2011.02.010. DOI: https://doi.org/10.1016/j.ecolmodel.2011.02.010

Quach Q. K., Chon T. S., Kim H., and Nguyen T. V. - One and two-individual movements of fish after chemical exposure, Journal of the Korean Physical Society, 63 (1) (2013) 18-27. https://doi.org/10.3938/jkps.63.18. DOI: https://doi.org/10.3938/jkps.63.18

Katz Y., Tunstrøm K., Ioannou C. C., Huepe C. and Couzin I. D. - Inferring the structure and dynamics of interactions in schooling fish, Proceedings of the National Academy of Sciences, 108 (46) (2011) 18720-18725. https://doi.org/10.1073/pnas.1107583108. DOI: https://doi.org/10.1073/pnas.1107583108