• Tran Van Cuong Department of Agricultural Processing and Food Technology, Tay Nguyen University, Vietnam
  • Nguyen Thi Thoa The Western Highland Agriculture and Forestry Science Institute, 53 Nguyen Luong Bang, Buon Ma Thuot, Daklak, Vietnam
  • NGUYEN THI THOA The Western Highland Agriculture and Forestry Science Institute, 53 Nguyen Luong Bang, Buon Ma Thuot, Daklak, Vietnam




Diabetes mellitus, glucose, hyperglycemia, VHSV, zebrafish


This study, we conducted a high glucose immersion protocol to induce hyperglycemic zebrafish, later on, with the aim to evaluate the relationship between hyperglycemia and VHSV infection using the zebrafish model. As the results, zebrafish immersed in a 2000 ppm glucose solution for 10 days, FBG has increased two times as compared to control. In addition, hyperglycemic zebrafish was more susceptible to the virus infection resulted in increased the viral mRNA expression levels as well as the viral copy number as 1.7 Log higher than control.


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Author Biography

Tran Van Cuong, Department of Agricultural Processing and Food Technology, Tay Nguyen University, Vietnam

Giảng viên Khoa Nông Lâm nghiệp, Đại học Tây Nguyên; Nghiên cứu sinh Tiến sĩ tại Khoa Khoa học và Công nghệ thực phẩm, Đại học Quốc gia Chonnam, Hàn Quốc.

Lĩnh vực quan tâm và nghiên cứu: Hoạt tính kháng oxy hóa, kháng khuẩn, kháng virus từ các hợp chất tự nhiên; Thực phẩm chức năng; Công nghệ sinh học thực phẩm (Công nghệ tế bào, vi sinh vật và công nghệ enzyme; tương tác protein, biểu hiện gen, cơ chế phân tử, con đường tín hiệu); Khoa học và Công nghệ chế biến các thực phẩm cơ (thịt).


Oka, T. et al. - Diet-induced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity. BMC Physiol. 10 (2010) 21–33.

Meguro, S., Hasumura, T. & Hase, T. - Body fat accumulation in zebrafish is induced by a diet rich in fat and reduced by supplementation with green tea extract. PLoS One 10 (2015) 1–12.

Garg, S. K., Maurer, H., Reed, K. & Selagamsetty, R. - Diabetes and cancer: Two diseases with obesity as a common risk factor. Diabetes, Obes. Metab. 16 (2014) 97–110.

Novoa, B. et al. - Zebrafish (Danio rerio) as a model for the study of vaccination against viral haemorrhagic septicemia virus (VHSV). Vaccine 24 (2006) 5806–5816.

Kim, S. H., Guo, T. C., Vakharia, V. N. & Evensen, Ø. - Specific nucleotides at the 3’-terminal promoter of viral hemorrhagic septicemia virus are important for virulence in vitro and in vivo. Virology 476 (2015) 226–232.

Trede, N. S., Langenau, D. M., Traver, D., Look, A. T. & Zon, L. I. - The use of zebrafish to understand immunity. Immunity 20 (2004) 367–379.

Sullivan, C. & Kim, C. H. - Zebrafish as a model for infectious disease and immune function. Fish Shellfish Immunol. 25 (2008) 341–350.

Seth, A., Stemple, D. L. & Barroso, I. - The emerging use of zebrafish to model metabolic disease. Dis. Model. Mech. 6 (2013) 1080–1088.

Zang, L., Maddison, L. A. & Chen, W. - Zebrafish as a Model for Obesity and Diabetes. Front. Cell Dev. Biol. 6 (2018) 1–13.

Carradice, D. & Lieschke, G. J. - Zebrafish in hematology: Sushi or science? Blood 111 (2008) 3331–3342.

Medina, C. & Royo, J. L. - Zebrafish as a model organism to study host-pathogen interactions. Methods 62 (2013) 241–245.

Feng, C.-W. et al. - Effects of 6-Hydroxydopamine Exposure on Motor Activity and Biochemical Expression in Zebrafish ( Danio Rerio ) Larvae. Zebrafish 11 (2014) 227–239.

Hachicho, N. et al. - Body mass parameters, lipid profiles and protein contents of zebrafish embryos and effects of 2,4-dinitrophenol exposure. PLoS One 10 (2015) 1–19.

Zang, L., Shimada, Y. & Nishimura, N. - Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus. Sci. Rep. 7 (2017) 1–11.

Capiotti, K. M. et al. - Persistent impaired glucose metabolism in a zebrafish hyperglycemia model. Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 171 (2014) 58–65.

Connaughton, V. P., Baker, C., Fonde, L., Gerardi, E. & Slack, C. - Alternate Immersion in an External Glucose Solution Differentially Affects Blood Sugar Values in Older Versus Younger Zebrafish Adults. Zebrafish 13 (2016) 87–94.

Encinas, P. et al. - Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach. BMC Genomics 11 (2010) 518–533.

Yang, D. et al. - Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nat. Commun. 9 (2018) 3052–3063.

Jung, S. H., Kim, Y. S., Lee, Y. R. & Kim, J. S. - High glucose-induced changes in hyaloid-retinal vessels during early ocular development of zebrafish: A short-term animal model of diabetic retinopathy. Br. J. Pharmacol. 173 (2016) 15–26.

Castro, R. et al. - Early Immune Responses in Rainbow Trout Liver upon Viral Hemorrhagic Septicemia Virus (VHSV) Infection. PLoS One 9 (2014) e111084.

Chen, L., Chen, R., Wang, H. & Liang, F. - Mechanisms Linking Inflammation to Insulin Resistance. Int. J. Endocrinol. 2015 (2015) 1–9.

Rehman, K. & Akash, M. S. H. - Mechanisms of inflammatory responses and development of insulin resistance: How are they interlinked? J. Biomed. Sci. 23 (2016) 1–18.

Chen, L. et al. - Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 9 (2018) 7204–7218.

Gupta, S. et al. - Analysis of inflammatory cytokine and TLR expression levels in Type 2 Diabetes with complications. Sci. Rep. 7 (2017) 1–10.

Memon, A. A. et al. - The association between cytokines and insulin sensitivity in Iraqi immigrants and native Swedes. BMJ Open 3 (2013) 1–7.

Tafalla, C., Coll, J. & Secombes, C. J. - Expression of genes related to the early immune response in rainbow trout (Oncorhynchus mykiss) after viral haemorrhagic septicemia virus (VHSV) infection. Dev. Comp. Immunol. 29 (2005) 615–626.

McBeath, A. J. A., Snow, M., Secombes, C. J., Ellis, A. E. & Collet, B. - Expression kinetics of interferon and interferon-induced genes in Atlantic salmon (Salmo salar) following infection with infectious pancreatic necrosis virus and infectious salmon anaemia virus. Fish Shellfish Immunol. 22 (2007) 230–241.

Laybutt, D. R. et al. - Endoplasmic reticulum stress contributes to beta cell apoptosis in type 2 diabetes. Diabetologia 50 (2007) 752–763.

Shan, B. et al. - The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity. Nat. Immunol. 18 (2017).

Cha, I. S. et al. - Heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with Streptococcus parauberis. Fish Shellfish Immunol. 34 (2013) 1455–1462.




How to Cite

Cuong, T. V., Thoa, N. T., & THOA, N. T. (2020). EFFECT OF HIGH GLUCOSE-INDUCED HYPERGLYCEMIA ON VIRAL HAEMORRHAGIC SEPTICAEMIA VIRUS (VHSV) INFECTION IN ADULT ZEBRAFISH. Vietnam Journal of Science and Technology, 58(1), 1. https://doi.org/10.15625/2525-2518/58/1/13530



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