Optimization of medium components for the production of ectoine by a halophilic bacterium isolated from Can Gio mangrove

Doan Van Thuoc; Tran Thi Hien

doi:10.15625/2525-2518/57/2/12979

Optimization of medium components for the production of ectoine by a halophilic bacterium isolated from Can Gio mangrove

Doan Van Thuoc, Tran Thi Hien

Author affiliations

Authors

Doan Van Thuoc Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, 136 XuanThuy, Cau Giay, Ha Noi, Viet Nam
Tran Thi Hien Department of Biotechnology and Microbiology, Faculty of Biology, Hanoi National University of Education, 136 XuanThuy, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/57/2/12979

Keywords:

ectoine, mangrove, halophilic bacteria, D209

Abstract

The effect of different carbon and nitrogen sources on growth of producer strain was investigated. Sucrose and glucose were found to be suitable carbon sources, and monosodium glutamate was favorable nitrogen source for bacterial cell growth. Optimum salt concentrations for bacterial growth was ranged from 4 to 6%, whereas, NaCl concentrations from 12 to 15% found to be good for ectoine accumulation. Two-step fed-batch fermentation was then designed, biomass and ectoine content were significant increased, maximum CDW of 25 g/l and ectoine content of 10.3% were obtained.

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References

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Published

05-04-2019

How to Cite

[1]

D. V. Thuoc and T. T. Hien, “Optimization of medium components for the production of ectoine by a halophilic bacterium isolated from Can Gio mangrove”, Vietnam J. Sci. Technol., vol. 57, no. 2, pp. 146–154, Apr. 2019.

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Issue

Vol. 57 No. 2 (2019)

Section

Natural Products

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