DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Yangia sp. ND199

Authors

  • Truong Huu Phong
  • Dang Minh Khuong
  • Duong Van Hop
  • Doan Van Thuoc

DOI:

https://doi.org/10.15625/0866-708X/55/2/8677

Keywords:

Yangia sp. ND199, fed batch fermentation, PHB production, fructose

Abstract

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         

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Author Biography

Truong Huu Phong

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Published

2017-04-17

Issue

Section

Natural Products