Clerodendrum japonicum (Thunb.) Sweet leaf extract supported nanosilver particles: Characterization, antioxidant and antibacterial activity: Clerodendrum japonicum nanosilver particles: antioxidant and antibacterial activity

Deepak Rabha; Himangshu Sarma; Manjit Borah; Vivek Ghose; Dharmeswar  Barman; Someswar Rao; Hemanta Kumar Sarma; Rajlakshmi Devi; Dulal Chandra Boruah

doi:10.15625/2525-2518/19376

Author affiliations

Authors

Deepak Rabha Department of Botany, Goalpara College, Goalpara-783101, Assam, India https://orcid.org/0000-0001-5973-9828
Himangshu Sarma Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati- 781035, Assam, India https://orcid.org/0000-0002-8585-8182
Manjit Borah Department of Physics, Goalpara College, Goalpara-783101, Assam, India
Vivek Ghose Mater in Science
Dharmeswar Barman Department of Botany, Goalpara College, Goalpara-783101, Assam, India
Someswar Rao PhD
Hemanta Kumar Sarma Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati- 781035, Assam, India https://orcid.org/0000-0003-2632-4903
Rajlakshmi Devi Department of Pharmaceutical Sciences, Faculty of Science & Engineering, Dibrugarh University, Dibrugarh, 786004, Assam, India
Dulal Chandra Boruah PhD https://orcid.org/0000-0001-7423-1555

DOI:

https://doi.org/10.15625/2525-2518/19376

Keywords:

green synthesis, silver nanoparticles, nanotherapeutics

Abstract

Silver nanoparticles have been synthesized in numerous ways, due to their diverse applications, including green procedures that used an extract of plants for the reduction of metal ions. This study delineates the green synthesis and characterization of silver nanoparticles (AgNPs) from the leaf extract of Clerodendrum japonicum (Thunb.) Sweet (CJ) and assesses the antioxidant as well as antibacterial characteristics. The biosynthesis of AgNPs was accomplished by reacting the aqueous leaf extract of the plant with a solution of silver nitrate (AgNO₃). The creation of AgNPs was indicated by the visual colour change of the reaction concoction from golden to deep brown and the absorption peak at 442 nanometer (nm) in the ultraviolet-visible spectroscopy. The Field Emission Scanning Electron Microscope (FESEM) and the High-Resolution Transmission Electron Microscope (HRTEM) images have revealed the generated AgNPs as spherical and oblate in shape which is 20-40 nm in size, whereas X-Ray power Diffraction evaluation revealed the crystalline feature. The existence of functional groups of synthesized AgNPs was detected by the Fourier Transform Infra-Red (FTIR) spectrum. The synthesized AgNPs showed free radical scavenging activity using 2, 2-Diphenyl-1-picryl hydrazyl assay with IC₅₀ value, 7.02±1 μg/mL. The antibacterial assay showed an effective activity towards E. Coli and S. aureus by developing a well-defined zone of inhibition. The results of this study accentuate the biomedical potential of the above-mentioned plant, though further research is needed to implement it in clinical practice.

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