Preparation and potential of nanoparticles containing curcuminoids to control fungal diseases in tropical fruits

Le Dang Quang; Nguyen Tuan Anh; Tran Quang De; Nguyen Thi Thu Trang; Vu Xuan Minh; Nguyen Cuong Quoc; Vo T. Kieu Anh; Bui Van Cuong; Tran Dai Lam; Tran Thanh Men; Do Tan Khang; Nguyen Trong Tuan

doi:10.15625/2525-2518/17620

Author affiliations

Authors

Le Dang Quang Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0001-7203-8081
Nguyen Tuan Anh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Tran Quang De College of Natural Sciences, Can Tho University, Campus II, 3/2 street, Xuan Khanh, Ninh Kieu, Can Tho city, Viet Nam
Nguyen Thi Thu Trang Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Vu Xuan Minh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Cuong Quoc College of Natural Sciences, Can Tho University, Campus II, 3/2 street, Xuan Khanh, Ninh Kieu, Can Tho city, Viet Nam
Vo T. Kieu Anh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Bui Van Cuong Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Tran Dai Lam Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Tran Thanh Men College of Natural Sciences, Can Tho University, Campus II, 3/2 street, Xuan Khanh, Ninh Kieu, Can Tho city, Vietnam
Do Tan Khang Institute of Food and Biotechnology, Can Tho University, Campus II, 3/2 street, Xuan Khanh, Ninh Kieu, Can Tho, Viet Nam
Nguyen Trong Tuan College of Natural Sciences, Can Tho University, Campus II, 3/2 street, Xuan Khanh, Ninh Kieu, Can Tho city, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/17620

Keywords:

anthracnose, Colletotrichum gloeosporioides, Colletotrichum orbiculare, curcuminoids, turmeric extract

Abstract

Colletotrichum species cause anthracnose in various tropical crops in both cultivation and postharvest periods. The current synthetic fungicides to treat anthracnose sometime show toxic effects for farmers, residues on foods, and environmental impacts. In this work, turmeric extract containing curcuminoids was used as an active ingredient and formulated into a nanoemulsion to control anthracnose in red pepper and tomato fruits. The nanoparticles containing turmeric extract (25 %) were successfully prepared by encapsulation using Tween-80 and PEG 400 as emulsifiers. Out of the three main curcuminoids in the turmeric extract, BDMC was determined as the most abundant constituent via HPLC analysis. Turmeric nanoparticles showed an average particle size of 203 nm, polydispersity index (PI) of 0.322, and zeta potential of -92.7 mV. In the in vitro antifungal bioassay, this nanoformulation significantly inhibited the mycelial growth of Colletotrichum gloeosporioides and Colletotrichum orbiculare in a dose-dependent manner. Red pepper anthracnose was also consistently suppressed by turmeric nanoparticles but the tomato anthracnose was resistant to this formulation at 10 mg/mL in vivo. The study results proved the effectiveness of turmeric nanoparticles to control red pepper anthracnose in postharvest preservation and suggested developing the innovative nanoformulation as a green fungicide.

Downloads

Download data is not yet available.

References

Czaja, K., Góralczyk, K., Struciński, P., Hernik, A., Korcz, W., Minorczyk, M., Łyczewska, M., Ludwicki, J.K., - Biopesticides-towards increased consumer safety in the European Union, Pest Manag. Sci. 71 (2015) 3–6. DOI: https://doi.org/10.1002/ps.3829

Sparks, T. C., Wessels, F. J., Lorsbach, B. A., Nugent, B. M., Watson, G. B. - The new age of insecticide discovery-the crop protection industry and the impact of natural products, Pesticide biochemistry and physiology. 161 (2019) 12-22. DOI: https://doi.org/10.1016/j.pestbp.2019.09.002

Nuruzzaman, M., Rahman, M.M., Liu, Y., Naidu, R. - Nanoencapsulation, Nano-guard for Pesticides: A New Window for Safe Application, J Agric Food Chem. 64 (2016) 1447–1483. DOI: https://doi.org/10.1021/acs.jafc.5b05214

Pascoli, M., Jacques, M.T., Agarrayua, D.A., Avila, D.S., Lima, R., Fraceto, L.F. - Neem oil based nanopesticide as an environmentally-friendly formulation for applications in sustainable agriculture: An ecotoxicological perspective, Science of The Total Environment. 677 (2019) 57–67. DOI: https://doi.org/10.1016/j.scitotenv.2019.04.345

Pavela, R., Benelli, G., Pavoni, L., Bonacucina, G., Cespi, M., Cianfaglione, K., Bajalan, I., Morshedloo, M.R., Lupidi, G., Romano, D., Canale, A., Maggi, F. - Microemulsions for delivery of Apiaceae essential oils-Towards highly effective and eco-friendly mosquito larvicides?, Industrial Crops and Products. 129 (2019) 631–640. DOI: https://doi.org/10.1016/j.indcrop.2018.11.073

Li, Yi, Zhao, R., Li, Yan, Zhou, Z. - Limonin enhances the antifungal activity of eugenol nanoemulsion against Penicillium italicum in vitro and in vivo tests, Microorganisms. 9 (2021) 969. DOI: https://doi.org/10.3390/microorganisms9050969

Ahmad, N., Ahmad, R., Al-Qudaihi, A., Edrees Alaseel, S., Zuhair Fita, I., Saifuddin Khalid, M., Hyder Pottoo, F. - Preparation of a novel curcumin nanoemulsion by ultrasonication and its comparative effects in wound healing and the treatment of inflammation, RSC Advances. 9 (2019) 20192–20206. DOI: https://doi.org/10.1039/C9RA03102B

Bhawana, Basniwal, R.K., Buttar, H.S., Jain, V.K., Jain, N. - Curcumin nanoparticles: preparation, characterization, and antimicrobial study, J. Agric. Food Chem. 59 (2011) 2056–2061. DOI: https://doi.org/10.1021/jf104402t

Akter, J., Amzad Hossain, M., Sano, A., Takara, K., Islam, Z., Hou, D. X. - Antifungal activity of various species and strains of turmeric (Curcuma spp.) against Fusarium solani sensu lato, Pharmaceutical Chemistry Journal 52 (2018) 320-325. DOI: https://doi.org/10.1007/s11094-018-1815-4

Akter, J., Islam, M.Z., Takara, K., Hossain, M.A., Gima, S. and Hou, D.X. - Plant growth inhibitors in turmeric (Curcuma longa) and their effects on Bidens pilosa, Weed Biology and Management. 18 (2018) 136-145. DOI: https://doi.org/10.1111/wbm.12157

Rex, B., Prabhu, S., & Kumar, J. S. - Antifungal efficacies of plant extracts against Alternaria solani (Ellis and Martin) jones and groutunder in vitro condition, Annals of Phytomedicine. 8 (2019) 1-5. DOI: https://doi.org/10.21276/ap.2019.8.1.19

Yang, C., Lu, J. H., Xu, M. T., Shi, X. C., Song, Z. W., Chen, T. M., Wang, S. Y. - Evaluation of chitosan coatings enriched with turmeric and green tea extracts on postharvest preservation of strawberries, LWT (2022) 113551. DOI: https://doi.org/10.1016/j.lwt.2022.113551

Bordoh, P. K., Ali, A., Dickinson, M., and Siddiqui, Y. - Antimicrobial effect of rhizome and medicinal herb extract in controlling postharvest anthracnose of dragon fruit and their possible phytotoxicity, Scientia Horticulturae. 265 (2020) 109249. DOI: https://doi.org/10.1016/j.scienta.2020.109249

Bui, V.C., Le, T.T., Nguyen, T.H., Pham, N.T., Vu, H.D., Nguyen, X.C., De Tran, Q., Hoang, T., Le Dang, Q., Lam, T.D. - Curcumin-removed turmeric oleoresin nano-emulsion as a novel botanical fungicide to control anthracnose ( Colletotrichum gloeosporioides ) in litchi, Green Processing and Synthesis. 10 (2021) 729–741. DOI: https://doi.org/10.1515/gps-2021-0071

Do, H.T.T., Nguyen, T.H., Nghiem, T.D., Nguyen, H.T., Choi, G.J., Ho, C.T., Le Dang, Q. - Phytochemical constituents and extracts of the roots of Scutellaria baicalensis exhibit in vitro and in vivo control efficacy against various phytopathogenic microorganisms, South African Journal of Botany. 142 (2021) 1–11. DOI: https://doi.org/10.1016/j.sajb.2021.05.034

Tan, T.N., Trung, H.T., Le Dang, Q., Thi, H.V., Vu, H.D., Ngoc, T.N., Thi Do, H.T., Nguyen, T.H., Quang, D.N., Tran Dinh, T. - Characterization and antifungal activity of limonoid constituents isolated from Meliaceae plants Melia dubia, Aphanamixis polystachya, and Swietenia macrophylla against plant pathogenic fungi in vitro, Journal of Chemistry. 2021 (2021) 1–12. DOI: https://doi.org/10.1155/2021/4153790

Meng, R., Wu, Z., Xie, Q.-T., Cheng, J.-S., Zhang, B. - Preparation and characterization of zein/carboxymethyl dextrin nanoparticles to encapsulate curcumin: Physicochemical stability, antioxidant activity and controlled release properties, Food Chemistry. 340 (2021) 127893. DOI: https://doi.org/10.1016/j.foodchem.2020.127893

Sari, T.P., Mann, B., Kumar, R., Singh, R.R.B., Sharma, R., Bhardwaj, M., Athira, S. - Preparation and characterization of nanoemulsion encapsulating curcumin, Food Hydrocolloids. 43 (2015) 540–546. DOI: https://doi.org/10.1016/j.foodhyd.2014.07.011

Wuthi-Udomlert, M., Grisanapan, W., Luanratana, O., Caichompoo, W. - Antifungal activity of Curcuma longa grown in Thailand, The Southeast Asian journal of tropical medicine and public health. 31 (2000) 178-182.

Cho, J. Y., Choi, G. J., Lee, S. W., Jang, K. S., Lim, H. K., Lim, C. H., Kim, J. C. - Antifungal activity against Colletotrichum spp. of curcuminoids isolated from Curcuma longa L. rhizomes, Journal of microbiology and biotechnology. 16 (2000) 280-285.

Yang, R., Miao, J., Shen, Y., Cai, N., Wan, C., Zou, L., Chen, C., Chen, J. - Antifungal effect of cinnamaldehyde, eugenol and carvacrol nanoemulsion against Penicillium digitatum and application in postharvest preservation of citrus fruit, LWT. 141 (2021) 110924. DOI: https://doi.org/10.1016/j.lwt.2021.110924