Bioactive of betel leaves extract grown in Vinh Long province and its application to extend shelf-life of Da Xanh pomelo

Author affiliations

Authors

  • Tran Thanh Truc Institute of Food And Biotechnology, Can Tho University, 3/2 street, Ninh Kieu, Can Tho City, Viet Nam https://orcid.org/0000-0002-5476-5098
  • Phan Minh Trong Institute of Food And Biotechnology, Can Tho University, 3/2 street, Ninh Kieu, Can Tho City, Viet Nam https://orcid.org/0009-0001-2480-9424
  • To Nguyen Phuoc Mai Institute of Food And Biotechnology, Can Tho University, 3/2 street, Ninh Kieu, Can Tho City, Viet Nam https://orcid.org/0009-0009-8670-3199
  • Nguyen Van Muoi Institute of Food And Biotechnology, Can Tho University, 3/2 street, Ninh Kieu, Can Tho City, Viet Nam https://orcid.org/0000-0001-6550-4769

DOI:

https://doi.org/10.15625/2525-2518/18887

Keywords:

piper betel leaf extract, coating, Da Xanh pomelo, shelf-life, storage

Abstract

It is well known that plant extract is a safe additive for coating systems that can extend fruit shelf-life. In this study, bioactive compounds of betel leaves (Piper betle L.) grown in Vinh Long province were extracted, characterized, and proposed to use in the coating preparation for Da Xanh pomelo (Citrus maxima (Burn.) Merr.). Characteristic tests showed that piper betel leaves extract (PBE) had high phenolic content and antioxidant activities. It could also inhibit seven different strains of mould presented in Da Xanh peel. Thus, PBE proved to be a suitable additive to coating Da Xanh pomelo. PBE was then mixed in the coating preparation at different concentrations (150; 200; 250; 300, and 400 µg GAE/mL), and the fruits were then coated with the coating sample. After 15 days of storage, Da Xanh pomelo coated at 250 µg GAE/mL showed the least changes in ∆E value, weight loss, and total yeast and mould count. Extended storage test results also showed that Da Xanh pomelos coated with betel leaf extract-added preparation were stable after 30 days of storage at room condition.

Downloads

Download data is not yet available.

References

1. Ministry of Agriculture and Rural Development - Decision no: 1648/QĐ-BNN-TT July 17th, 2013 “Approval of the master plan for the key fruit tree cultivation area with concentrated cultivation and staggered planting orientation of some fruit trees in the Southwestern region by 2020”. https://sofri.org.vn/vi/thu-vien/detail/Quyet-dinh-1648-qd-bnn-tt-bo-nong-nghiep-va-phat-trien-nong-thon-71.html?download=2&id=0 (accessed 15 March 2023).

2. General Statistics Office - (2024). Statistical Yearbook 2024. https://www.nso.gov.vn/wp-content/uploads/2025/06/NGTK-Cuc-TK-2024_BQ.pdf (accessed 02 October 2025).

3. Nhan dan online - Planning and developing areas specializing in quality and effective Da Xanh pomelo cultivation. https://nhandan.vn/quy-hoach-phat-trien-vung-chuyen-canh-buoi-da-xanh-chat-luong-hieu-qua-post285464.html (accessed 15 March 2023).

4. Madhumita, M., Guha, P., Nag, A. - Bio-actives of betel leaf (Piper betle L.): A comprehensive review on extraction, isolation, characterization, and biological activity. Phytother. Res. 34 (10) (2020) 2609-2627. https://doi.org/10.1002/ptr.6715.

5. Tran V. T., Tran B. N., Nguyen N. H. C., Do N. N. H, Le K. P. - Recent applications of natural bioactive compounds from Piper betle (L.) leaves in food preservation, Food Control 154 (2023) 110026. https://doi.org/10.1016/j.foodcont.2023.110026.

6. Trang D. T. X., Linh T. C., Nhi N. T., Men T. T., Dinh P. K., and Tuan N. T. - Investigation of bioactivities of the extract from Premna serratifolia (L.) leaves, Can Tho Univ. J. Sci. 54 (9) (2018) 46-52. https://doi.org/10.22144/ctu.jvn.2018.159.

7. Mandal V., Mohan Y., and Hemalatha S. J. P. R. - Microwave assisted extraction-an innovative and promising extraction tool for medicinal plant research, Pharmacognosy reviews 1 (1) (2007) 7-18.

8. Ayesha S., Premakumari K. B., and Roukiya S. - Antioxidant activity and estimation of total phenolic content of Muntingia calabura by colorimetry, International Journal of ChemTech Research 2 (1) (2010) 205-208.

9. Chmelová D., Ondrejovič M., Havrlentová M., and Hozlár P. - Antioxidant activity in naked and hulled oat (Avena sativa L.) varieties. Journal of Microbiology, Biotechnology and Food Sciences 4 (Special issue 3) (2015) 63-65. https://doi.org/ 10.15414/jmbfs.2015.4.special3.63-65.

10. Nenadis N., Wang L. F., Tsimidou M., and Zhang H. Y. - Estimation of scavenging activity of phenolic compounds using the ABTS•+ assay, Journal of agricultural and food chemistry 52 (15) (2004) 4669-4674. https://doi.org/10.1021/jf0400056.

11. Soto C., Caballero E., Pérez E., and Zúñiga M. E. - Effect of extraction conditions on total phenolic content and antioxidant capacity of pretreated wild Peumus boldus leaves from Chile, Food and Bioproducts Processing 92 (3) (2014) 328-333. https://doi.org/10.1016/ j.fbp.2013.06.002.

12. Benzie I. F. F., and Strain J. J. - The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay, Analytical Biochemistry 239 (1) (1996) 70-76. https://doi.org/10.1006/abio.1996.0292.

13. Zhu Q. Y., Hackman R. M., Ensunsa J. L., Holt R. R., and Keen C. L. - Antioxidative activities of oolong tea, Journal of Agricultural and Food Chemistry 50 (23) (2002) 6929-6934. https://doi.org/10.1021/jf0206163.

14. Prieto P., Pineda M., and Aguilar M. - Spectrophotometric quantitation of antioxidant capacity through the formation of complex: Specific application to the determination of vitamin E, Analytical Biochemistry 269 (2) (1999) 337-341. https://doi.org/10.1006 /abio.1999.4019.

15. Alisi C. S., Onyeze G. O. C. - Nitric oxide scavenging ability of ethyl acetate fraction of methanolic leaf extracts of Chromolaena odorata (Linn.), African Journal of Biochemistry Research 2 (7) (2008) 145-150.

16. Gakuubi M. M., Maina A. W., and Wagacha J. M. - Antifungal activity of essential oil of Eucalyptus camaldulensis Dehnh. against selected Fusarium spp, International Journal of Microbiology (2017). https://doi.org/10.1155/2017/8761610.

17. Tam H. N. T., Tuoi N. T. K., and Toan H. T. - Isolation and identification of fungi on the peels of Da Xanh and Nam Roi pomelo growing in the Mekong Delta, Can Tho Univ. J. Sci. 57 (2021) 108-117. https://doi.org/10.22144/ctu.jsi.2021.012.

18. Lam N. D., Tuan D. V. - Preservation of some Hanoi’s citrus specialties by using of micro-emulsion fruit coatings, Proceedings of Int. Workshop on Agrl. & Bio-Systems Engineering, Agriculture Publishing House, 2009, pp. 259-267.

19. Muruganandam L., Krishna A., Reddy J., and Nirmala G. S. - Optimization studies on extraction of phytocomponents from betel leaves, Resource-Efficient Technology 3 (4) (2017) 385-393. https://doi.org/10.1016/ j.reffit.2017.02.007.

20. Tagrida M., and Benjakul S. - Ethanolic extract of Betel (Piper betle L.) and Chaphlu (Piper sarmentosum Roxb.) dechlorophyllized using sedimentation process: Production, characteristics, and antioxidant activities, J. Food Biochem. 44 (1) (2020) 13508. DOI: 10.1111/jfbc.13508.

21. Sebaugh J. L. - Guidelines for accurate EC50/IC50 estimation, Pharm. Stat. 10 (2) (2011) 128-134. DOI: 10.1002/pst.426.

22. Fidrianny I., Suhendy H., and Insanu M. - Correlation of phytochemical content with antioxydant potential of various sweet potato (Ipomoea batatas) in West Java, Indonesia, Asian Pac. J. Trop. Biomed. 8 (1) (2018) 25. DOI: 10.4103/2221-1691.221131.

23. Ali I., Khan F. G., Suri K. A., Gupta B. D., Satti N. K., Dutt P., Afrin F., Qazi G. N., and Khan I. A. - In vitro antifungal activity of hydroxychavicol isolated from Piper betle L., Ann. Clin. Microbiol. Antimicrob. 9 (1) (2010) 7. https://doi.org/10.1186/1476-0711-9-7.

24. Trakranrungsie N., Chatchawan-chonteera A., and Khunkitti W. - Ethnoveterinary study for antidermatophytic activity of Piper betle, Alpinia galanga and Allium ascalonicum extracts in vitro, Res. Vet. Sci. 84 (2008) 80-84. doi.org/10.1016/j.rvsc.2007.03.006.

25. Martha Perez Gutierrez R., Maria Neira Gonzalez A., and Hoyo-Vadillo C. - Alkaloids from piper: a review of its phytochemistry and pharmacology, Mini-Rev. Med. Chem. 13 (2) (2013) 163-193.

26. Paliyath G., Tiwari K., Sitbon C., and Whitaker B. D. - Biochemistry of fruits. Food biochemistry and food processing (2012) 531-553. https://doi.org/10.1002/ 9781118308035.ch27.

27. Putnik P., Bursać Kovačević D., Režek Jambrak A., Barba F. J., Cravotto G., Binello A., Lorenzo J. M., and Shpigelman A. - Innovative “green” and novel strategies for the extraction of bioactive added value compounds from citruswastes - A review, Molecules 22 (5) (2017) 22050680. https://doi.org/10.3390/molecules22050680.

28. Hoque M. M., Rattila S., Shishir M. A., Bari M. L., Inatsu Y., and Kawamoto S. - Antibacterial Activity of Ethanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens, Bangladesh J. Microbiol. 28 (2) (2011) 58-63. https://doi.org/ 10.3329/bjm.v28i2.11817.

29. Intellectual Property Office of Viet Nam - Geographical Indication Protection of “Ben Tre” for Da Xanh Pomelo. https://ipvietnam.gov.vn/phat-trien-chi-dan-ia-ly/asset_publisher/ SGA9PgvmYtWI/content/bao-ho-chi-dan-ia-ly-ben-tre-cho-san-pham-buoi-da-xa-1 (accessed 15 March 2023).

Downloads

Published

12-12-2025

How to Cite

[1]T. T. Tran, M. T. Phan, P. M. To Nguyen, and V. M. Nguyen, “Bioactive of betel leaves extract grown in Vinh Long province and its application to extend shelf-life of Da Xanh pomelo”, Vietnam J. Sci. Technol., vol. 63, no. 6, pp. 1083–1093, Dec. 2025.

Issue

Section

Natural Products

Similar Articles

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 > >> 

You may also start an advanced similarity search for this article.