Antioxidant, angiotensin converting enzyme inhibitory and anthelmintic activities of bark essential oil of Cinnamomum bejolghota (Buch.-Ham.) Sweet from North East India

Barnali Gogoi; Kashyap J. Tamuli; Snigdha Saikia; Neipihoi Lhouvum; Manobjyoti Bordoloi; Hemanta K. Sarma

doi:10.15625/2525-2518/17346

Author affiliations

Authors

Barnali Gogoi Department of Chemistry, Cotton University, Panbazar, Guwahati 781001, Assam, India
Kashyap J. Tamuli Department of Chemistry, Cotton University, Panbazar, Guwahati 781001, Assam, India
Snigdha Saikia Chemical Science and Technology Division, CSIR North East Institute of Science and Technology, Jorhat 785006, Assam, India
Neipihoi Lhouvum
Manobjyoti Bordoloi Chemical Science and Technology Division, CSIR North East Institute of Science and Technology, Jorhat 785006, Assam, India
Hemanta K. Sarma Pharmaceutical Sciences Department, Dibrugarh University, Dibrugarh 786004, Assam, India

DOI:

https://doi.org/10.15625/2525-2518/17346

Keywords:

Cinnamomum bejolghota, bark essential oil, antioxidant, angiotensin-converting enzyme inhibition, anthelmintic activity

Abstract

Cinnamomum bejolghota (Buch.-Ham.) Sweetisa traditional anthelmintic and cardiotonic medicinal plant. The essential oil extracted from the bark of C. bejolghota was characterized by gas chromatography-mass spectrometry and 28 constituents were identified. Monoterpenoids were found to be dominating chemical constituents with α-terpineol (18.57 %) as the major component of the essential oil. The antioxidant activity of the essential oil was evaluated by DPPH radical, β-carotene bleaching and reducing power assay methods. The essential oil showed in-vitro dose-response hindering activity against angiotensin-converting enzyme with an IC₅₀value of 33.43 ± 0.46 µg/mL.The essential oil also exhibited potent anthelmintic property in-vitro against Caenorhabditis elegans with an ED₅₀value of 80.53 ± 3.57 µg/mLin adult mortality assay. The inhibitory activity of essential oil against larval development assay showed an ED₅₀value of 72.40 ± 2.68 µg/mL against C. elegans larva. Further, the egg hatching assay using C. eleganseggs demonstrated an ED₅₀value of 68.27 ± 3.51 µg/mL with this essential oil. Thus, the ethnomedicinal claim has been validated for the bark essential oil, which provides an excellent scope for the development of the C. bejolghota bark essential oil as novel herbal antihypertensive and anthelmintic agents.

Downloads

Download data is not yet available.

References

Hedge S. N. - Conservation of North East Flora, Arunachal Forest News 18 (1&2) 5-26.

Gogoi B., Tamuli K. J., Neipihoi., Bordoloi M., Sharma H. K. - Isolation and Characterization of Chemical Constituents with in vitro Anti-hypertensive and Anthelmintic Activities of Cinnamomum bejolghota (Buch.-Ham.) Sweet leaves: An Ethno Medicinal Plant of North East India, S. Afr. J. Bot. 140 (2014) S1:S427-434. doi.org/10.1016/j.sajb.2021.04.004.

Atiphasaworn P., Monggoot S., Pripdeevech P. - Chemical composition, antibacterial and antifungal activities of Cinamomum bejolghota bark oil from Thailand, J. App. Pharm. Sci. 7 (2017) 069-073. doi: 10.7324/JAPS.2017.70409.

Insung A., Pumnuan J., Mahakittikun V., Wangapai T. - Effectiveness of essential oils of medicinal plants at reducing the amounts of allergen produced by the European house dust mite, Dermatophagoides pteronyssinus (Trouessart), J. Acarol Soc. of Jpn. 25 (2016) S179-S184. doi:10.2300/acari.25.Suppl_179.

Wannissorn B., Jarikasem S., Siriwangchai T., Thubthimthed S. - Antibacterial properties of essential oils from Thai medicinal plants, Fitoterapia 5 (2005) 233-236. doi:10.1016/ j.fitote.2004.12.009.

Chen Z. Y., Peng C., Jiao R., Wong Y. M., Yang N., Huang Y. - Antihypertensive nutraceuticals and functional foods, J. Agric. Food. Chem. 57 (2009) 4485-4499. doi.org/ 10.1021/jf900803r.

Allegrone G., Belliardo F., Cabella P. - Comparison of volatile concentrations in hand-squeezed juices of four different lemon varieties, J. Agric. Food Chem. 54 (2006) 1844-1848. doi:10.1021/jf051206s.

Skeggs L. T., Kahn J. E., Shumway N. P. - The preparation and function of the angiotensin I-converting enzyme, J. Exp. Med. 103 (1956) 295-299. doi:10.1084/jem. 103.3.295.

Kapel R., Rahhou E., Lecouturier D., Guillochon D, Dhulster P. - Characterization of an antihypertensive peptide from an Alfalfa white protein hydrolysate produced by a continuous enzymatic membrane reactor, Process Biochem 41 (2006) 1961-1966. doi.org/10.1016/j.procbio.2006.04.019.

Hajji M., Masmoudi O., Souissi N., Triki Y., Kammoun S., Nasri M. - Chemical composition, angiotensin I-converting enzyme (ACE) inhibitor, antioxidant and antimicrobial activities of the essential oil from Periploca laevigata root barks, Food Chem. 121 (2010)724-731. doi.org/10.1016/j.foodchem.2010.01.021.

Mohamed B. M., Raﬁk B., Lotﬁ R., Ali B., Mokhtar G. - Chemical composition, angiotensin I-converting enzyme (ACE) inhibitory, antioxidant and antimicrobial activities of the essential oil from south Tunisian Ajuga pseudoiva Rob. Lamiaceae, Process Biochem 48 (2013) 723-729. doi.org/10.1016/j.procbio.2013.02.022.

Kris-Etherton P. M., Hecker K. D., Bonanome A., Coval S. M., Binkoski A. E., Hilpert K. F. - Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer, Am. J. Med. 113 (2002)71-88.doi: 10.1016/s0002-9343(01)00995-0.

Lahlou S., Interaminense L. F. L., Leal-Cardoso J. H., Duarte G. P. - Antihypertensive effects of the essential oil of Alpinia zerumbet and its main constituent, terpinen-4-ol, in DOCA-salt hypertensive conscious rats, Fundam Clin Pharmacol 17 (2003)323-330. doi:10.1046/j.1472-8206.2003.00150.x

Hammond J. A., Fielding D., Bishop S. C. - Prospects for plant anthelmintics in tropical veterinary medicine, Vet. Res. Commun. 21 (1997) 213-28. doi:10.1023/a: 1005884429253.

Grando T. H., Baldissera M. D., Gressler L. T., Sa M. F., Bortoluzzi B. N., Schafer A. S., Ebling R. C., Raffin R. P., Santos R. C. V., Stefani L. M., Vaucher R., Leal M. L. R., Monteiro S. G. -Melaleuca alternifolia anthelmintic activity in gerbils experimentally infected by Haemonchus contortus, ExpParasitol 170 (2016)177-183. doi:10.1016/ j.exppara.2016.09.004.

Sibomana J. P., Campeche A., Roberto J., Carvalho-Filho., Correa R. A., Duani H, Guimaraes V. P., Hilton J. F., Kassa B., Kumar R., Lee M. H., Loureiro C. M. C., Mazimba S., Mickael C., Rudol K. F., Oliveira J. S. - Schistosomiasis pulmonary arterial hypertension, Frontiers in Immunology 11 (2020) 608883.

Hoang T. B., Le M. H., Nguyen Q. C., Dinh T. T. T., Le T. T., Do T. S., Le T. T. H., Pham H. H. - Optimization of the enzyme assisted extraction of essential oil from the leaves and branches of Cinnamomum cassia using Box-Wilson method, Vietnam Journal of Science and Technology 55 (6) (2017) 690-697.doi: 10.15625/2525-2518/55/6/9525.

Ninh T. S., Nguyen D. D., Le T. A., Dinh T. T. T., Nguyen D. L., Tran T. T. - Essential oil from the aerial part of Orchidantha vietnamica k. larsen and its antimicrobial activity, Vietnam Journal of Science and Technology 60 (1) (2022)43-48.doi:10.15625/2525-2518/15867.

Ngo T. P., Dinh T. T. T., Do T. T., Do T. T. H., Le N. H., Nguyen T. A., Le M. H. - Volatile compounds and biological activities of essential oil of Globba pendula rhizomes collected at an Giang province, Vietnam Journal of Science and Technology 58 (4) (2020) 434-44. doi:10.15625/2525-2518/58/4/14856.

Neipihoi., Narzary B., Saikia S., Saikia S., Tamuli K. J., Sahoo R. K., Dutta D., Bordoloi M. - Anticancer and antimicrobial compounds from Croton caudatus Gieseler and Eurya acuminata DC: Two edible plants used in the traditional medicine of the Kuki tribes, Natural Product Research, 2020. doi: 10.1080/14786419.2020.1815737.

Tamuly C., Dutta P.P., Bordoloi M., Bora J. - Antifungal and Antioxidant Pyrrole Derivative from Piper pedicellatum, Natural Product Communications 8 (10) 1934578X1300801029

Saikia S., Tamuli K.J., Narzary B., Banik D., Bordoloi M. - Chemical characterization, antimicrobial activity, and cytotoxic activity of Mikania micrantha Kunth flower essential oil from North East India, Chemical Papers 74 (2020) 2515-2528.

Cao H., Li Z., Chen X. - QSRR study of GC retention indices of volatile compounds emitted from Mosla chinensis Maxim. by multiply linear regression, Chin. J. Chem. 29 (2011) 2187-2196.

Kovats E. - Gas chromatographic characterization of organic substances in the retention index system, Adv. in Chromatogr 1 (1965) 229-247.

Adams R. P. -Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry Allured Publishing Corporation, Carol Stream, IL, USA, 1995.

Kirby A. J., Schmidt R. J. - The antioxidant activity of Chinese herbs for eczema and placebo herbs, J. Ethnopharmacol 56 (1997)103-108.doi: 10.1016/s0378-8741(97)015109.

Nakamura Y., Yamamoto N., Sakai K., Okubo A., Yamazaki S., Takano T. - Purification and characterization of angiotensin I-converting-enzyme inhibitors from sour milk, J. Dairy Sci. 78 (1995) 777-783. doi.org/10.3168/jds.S0022-0302 (95)76689-9.

Viviane S. R., Wanderley D. S. - Caenorhabditis elegans as a model for the screening of anthelminthic compounds: Ultrastructural study of the effects of albendazole, Exp Parasitol 135 (2013) 1-8. doi:10.1016/j.exppara.2013.05.011.

Brenner S. - The genetics of Caenorhabditis elegans, Genetics. 77 (1) (1974) 71-94.

Atiphasaworn P., Monggoot S., Pripdeevech P. - Chemical composition, antibacterial and antifungal activities of Cinamomum bejolghotabark oil from Thailand, J. App. Pharm. 7 (2017) 69-73. doi:10.7324/JAPS.2017.70409.

Choudhury R., Ahmed A., Barthel P. A., Leclercq - Composition of the Bark and Flower Oils of Cinnamomum bejolghota (Buch.- Ham.) Sweet from two Locations of Assam, India, Journal of Essential Oil Research 10 (1998) 245-250. doi:10.1080/ 10412905.1998.9700896.

Baruah A., Nath S. C., Hazarika A., Sarma T. C. - Essential Oils of the Leaf, Stem Bark and Panicle of Cinnamomum bejolghota (Buch.-Ham.) Sweet, Journal of Essential Oil Research 9 (1997) 243-245. doi:10.1080/10412905.1997.9699472.

Kulisic T. A., Katalinic V., Milos M. - Use of different methods for testing antioxidative activity of oregano essential oil, Food Chem. 85 (2004) 633-640. doi.org/10.1016/ j.foodchem.2003.07.024.

Shimada K., Fujikawa K., Yahara K., Nakamura T. - Antioxidative properties of xanthum on the autoxidation of soybean oil in cyclodextrin emulsion, J. Agric. Food. Chem. 40 (1992) 945-948. doi: 10.3168/jds.S0022-0302 (95)76689-9.

Brand C., Ferrante A., Prager R. H., Riley T. V., Carson C. F., Finaly-Jones J. J. - The water-soluble components of the essential oil of Melaleuca alternifolia (tea tree oil) suppress the production of superoxide by human monocytes, but not neutrophils, activated in vitro, Inflamm Res. 50 (2001) 213-219. doi:10.1007/s000110050746.

Seol G. H., Kan P., Le H. S., Seol G. H. - Antioxidant activity of linalool in patients with carpal tunnel syndrome, BMC Neurology 16 (2016)17. doi:10.1186/s12883-016-0541-3.

Souza C. F., Baldissera M. D., Silva L. L., Geihs M., Baldisserotto B. - Is monoterpene terpinen-4-ol the compound responsible for the anesthetic and antioxidant activity of Melaleuca alternifolia essential oil (tea tree oil) in silver catfish, Aquaculture 486 (2018) 217-223. doi.org/10.1016/j.aquaculture.2017.12.025.

Nacim Z., Nahed F., Sami Z., Ali B., Aida K., Mohamed N., Ayadi M. A. - Chemical composition, angiotensin I-converting enzyme inhibitory, antioxidant and antimicrobial activities of essential oil of Tunisian Thymus algeriensisBoiss. et Reut. (Lamiaceae), Food and Bioprod Process 89 (2018) 257-265. doi.org/10.1016/j.fbp.2010.11.006.

Camargo S. B., Simoes L. O., de Azevedo Medeiros C. F., Jesus A de Melo., Fregoneze J. B., Evangelista A., Villarreal C. F., de Souza Araujo A. A., Quintans-Junior L. J., Silva D. F. - Antihypertensive potential of linalool and linalool complexed with β-cyclodextrin, BiochemPharmacol 151 (2018) 38-46. doi:10.1016/j.bcp.2018.02.014.

Anjos P. J., Lima A. O., Cunha P. S., De Sousa, D. P., Onofre A. S., Ribeiro T. P., Medeiros I. A., Antoniolli A. R., Quintans-Junior L. J., Santosa M. R. - Cardiovascular effects induced by linalool in normotensive and hypertensive rats, Z Naturforsch 68 (2013) 181-190. doi:10.5560/ZNC.2013.68c0181.

Kang P., Seol G. H. - Linalool elicits vasorelaxation of mouse aortae through activation of guanylyl cyclase and K(+) channels, J. Pharm. Pharmacol 67 (2015) 714-719. doi:10.1111/jphp.12359.

Sabino C. K., Ferreria-Filho E. S., Mendes M. B., Da SilvaFilho J. C.- Cardiovascular effects induced by α-terpineol in hypertensive rats, Flavour Frag. J. 28 (2013) 333-339. doi.org/10.1002/ffj.3159.

Katiki L. M., Ferreira J. F. S., Zajac A. M., Masler C., Lindsay D. S., Chagas A. C. S., Amarante A. F. T. - Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use, Vet Parasitol 182 (2011) 264-268.

Burns A. R., Luciani G. M., Musso G., Bagg R., Yeo M., Zhang Y., Rajendran L., Glavin J., Hunter R., Redman E., Stasiuk S., Schertzberg M., Angus McQuibban G., Caffrey C. R., Cutler S. R., Tyers M., Giaever G., Nislow C., Fraser A. G., MacRae C. A., Gilleard J., Roy P. J. - Caenorhabditis elegans is a useful model for anthelmintic discovery, Nat. Communn. 6 (2015) 7485. doi:10.1038/ncomms8485.

Khan A. S. - Medicinally Important Trees. Springer International Publishing, 2017, ISBN: 978-3-319-56777-8.

Katiki L., Evangelista A., Canova E., Piza A., Fornazari B., Araujo R., Bueno M. - Anthelmintic activity of anethole, carvone, carvacrol, thymol, linalool, limonene, eucalyptol, vanillin, cinnamaldehyde and eugenol in in vitro tests, J. Med. Plants 80 (2014) 1415. doi:10.1055/s-0034-1394672.

Grando T. H., Baldissera M. D., Gressler L. T., Sa M. F., Bortoluzzi B.N., Schafer A. S., Ebling R. C., Raffin R. P., Santos R. C. V., Stefani L. M., Vaucher R., Leal M. L. R., Monteiro S. G. - Melaleuca alternifolia anthelmintic activity in gerbils experimentally infected by Haemonchus contortus, ExpParasitol 170 (2016) 177-183 doi:10.1016/ j.exppara.2016.09.004.

Fernandez C. M. M., Da Rosa M. F., Fernandez A. C. A. M., Bortolucci W. D. C., Ferreira F. B. P., Linde G. A., and Gazim Z. C. - Essential oil and fractions isolated of Laurel to control adults and larvae of cattle ticks, Natural Products Research 34 (5) (2018) 731-735. PMID: 30445842. doi:10.1080/14786419.2018.1495637.

Adams R. P., Gonzalez Elizondo M. S., Gonzalez Elizondo M., Slinkman E. - DNA fingerprinting and terpenoid analysis of Juniperus blancoi var. huehuentensis (Cupressaceae), a new subalpine variety from Durango, Mexico. Biochem. Syst. Ecol. 34 (2006) 205-211. doi:10.1016/j.bse.2005.11.004.

Souza C. F., Baldissera M. D., Silva L. L., Geihs M., Baldisserotto B. - Is monoterpene terpinen-4-ol the compound responsible for the anesthetic and antioxidant activity of Melaleuca alternifolia essential oil (tea tree oil) in silver catfish, Aquaculture 20 (2010) 175-179. doi:10.1021/acs.jafc.7b01350.

Fadel H. H. M., Abdel Mageed M. A., Abdel Samad A. K. M. E. A., Lotfy S. N. - Cocoa substitute: Evaluation of sensory qualities and flavour stability, Eur. Food Res. Technol. 223 (2006) 125-131. 10.1007/s00217-005-0162-3

Babushok V. I., Linstrom P. J., Zenkevich I. G. - Retention Indices for Frequently Reported Compounds of Plant Essential Oils, J. Phys. Chem. Ref. Data. 40 (2011) 043101-47. doi:10.1063/1.3653552.

Juliani H. R., Koroch A. R., Juliani H. R., Trippi V. S., Zygadlo J. A. - Intraespecific variation in leaf oils of Lippia junelliana (mold.) tronc, Biochem. Syst. Ecol. 30 (2002) 163-170. doi:10.1016/S0305-1978(01)00067-9.

Baranauskiene R., Venskutonis R. P., Demyttenaere J. C. R. - Sensory and instrumental evaluation of catnip (Nepeta cataria L.) aroma, J. Agric. Food Chem. 51 (2003) 3840-3848. doi:10.1021/jf021187b.

Mevy J. P., Bessiere J. M., Gref S., Zombre G., Viano J. - Composition of the volatile oil from the leaves of Ximenia americanaL, Biochem. Syst. Ecol. 34 (2006) 549-553. doi:10.1016/j.bse.2006.01.007.

Adams R. P., Morris J. A., Pandey R. N., Schwarzbach A. E. - Cryptic speciation between Juniperus deltoides and Juniperus oxycedrus (Cupressaceae) in the Mediterranean, Biochem. Syst. Ecol 33 (2005)771-787. doi:10.1016/j.bse.2005.01.001.

Lucero M. E., Fredrickson E. L., Estell R. E., Morrison A. A., Richman D. B. -Volatile composition of Gutierrezia sarothrae (Broom Snakeweed) as determined by Steam Distillation and Solid Phase Microextraction, J. Essent. Oil Res. 18(2006)121-125.doi: 10.1080/10412905.2006.9699039.

Scrivanti L. R., Anton A. M., Zygadlo J. A. - Essential oil composition of Bothriochloa kuntze (Poaceae) from South America and their chemotaxonomy, Biochem, Systematics Ecol. 37 (2009) 206-213. doi:10.1016/j.bse.2009.03.009.

de Kraker J. W., Schurink M., Franssen M. C. R., Konig W. A., de Groot A., Bouwmeester H. J. - Hydroxylation of sesquiterpenes by enzymes from chicory (Cichorium intybus L.) roots, Tetrahedron 59 (2003) 409-418. doi:10.1016/S0040-4020(02)01479.

Xu X., Tang Z., Liang Y. - Comparative analysis of plant essential oils by GC-MS coupled with integrated chemo metric resolution methods, Anal. Methods 2 (2010) 359-367. doi:10.1039/B9AY00213H.

Magalhaes L. A. M., Lima M., Marques M. O. M., Facanali R., Pinto A. C., Tadei W. P. - Chemical composition and larvicidal activity against Aedes aegypti Larvae of essential oils from four Guarea species, Molecules 15 (2010) 5734-5741. doi.org/10.3390/ molecules15085734.

Santos P. R., Moreira D. L., Guimaraes E. F., Kaplan M. A. C. - Essential oil analysis of 10 piperaceae species from the Brazilian Atlantic forest, Phytochemistry 58 (2001) 547-551. doi:10.1016/s0031-9422(01)00290-4.

Zizovic I., Stamenic M., Ivanovic J., Orlovic A., Ristic M., Djordjevic S., Petrovic S.D., Skala D. - Supercritical carbon dioxide extraction of sesquiterpenes from valerian root, J. Supercrit. Fluids. 43 (2007) 249-258. doi:10.1016/j.supflu.2007.05.007.

Nogueira P. C. L., Bittrich V., Shepherd G. J., Lopes A. V., Marsaioli A. J. - The ecological and taxonomic importance of flower volatiles of Clusia species (Guttiferae), Phytochemistry 56 (2001) 443-452. doi.org/10.1016/S0031-9422.