Conjugation of tetracycline with carrier proteins and production of its polyclonal antibody for the development of rapid test

Ngo Thu Huong; Hoang Thi Huyen; Truong Quoc Phong

doi:10.15625/2525-2518/17325

Author affiliations

Authors

Ngo Thu Huong Center for Research and Development of Vaccines and Biologicals, No.135 Lo Duc Street, Hai Ba Trung, Ha Noi, Viet Nam
Hoang Thi Huyen School of Biotechnology and Food Technology, University of Science and Technology, No.1 Dai Co Viet Street, Hai Ba Trung, Ha Noi, Viet Nam
Truong Quoc Phong School of Biotechnology and Food Technology, University of Science and Technology, No.1 Dai Co Viet Street, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/17325

Keywords:

tetracycline, conjugation, polyclonal antibody, rapid test

Abstract

Tetracycline (TC) is a broad-spectrum antibiotic used in the treatment of several infectious diseases. The excessive use of tetracycline antibiotics, especially tetracyclines in livestock, has raised concerns about residues of tetracycline antibiotics in food that may affect consumers' health. It is necessary to develop a rapid test strip for detecting tetracycline antibiotic residues in food and animal feed. This paper presents the suitable conditions for the conjugation of tetracycline to BSA or KLH: molar ratio of TC:BSA/KLH was 25:1, formaldehyde concentration of 5 %, temperature of 4^oC and reaction time of 4 hours. The KLH-TC conjugate was used to successfully raise polyclonal antibodies against TC in rabbits. The anti-TC polyclonal antibodies were purified and could be used for the development of the lateral flow immunoassay test strip for the detection of tetracycline.

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