Development of novel, simple and low–cost microfluidic platform for supporting 3D dynamic cell culture

Nguyen Thanh Duong Nguyen; Thu Uyen Pham; Tran Dinh Thiet

doi:10.15625/2525-2518/16735

Author affiliations

Authors

Nguyen Thanh Duong Nguyen Graduate University of Science and Technology (GUST), Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Thu Uyen Pham Institute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Tran Dinh Thiet High School of Education Sciences, VNU – University of Education, 144 Xuan Thuy, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16735

Keywords:

3D cancer spheroid, microfluidics, laser engraving, dynamic cell culture

Abstract

Cell culture models more accurately would be of significant value to the medical field and pharmaceutical industry. To achieve this goal, microfluidic cell culture platforms are created and improved for modeling the native cell microenvironment because they can precisely reconstruct in vivo cellular behavior. In this study, a 3D low-cost microfluidic device is used to compare the difference between the static and dynamic environment in 3D cell culture. Cells were seeded in the microfluidic device, and to produce the fluidic flow, the pump was used with the set speed was 0.045ml/min. In 3D cell culture, the viability of cells was monitored by size growth of the spheroids for 7 days. All systems were designed and optimized without leakage of the medium. In the results, the 3D dynamic condition showed a faster increase in size than in the static condition. Overall, the study was prepared for microfluidic platforms with low-cost and simple settings. Moreover, the usage of 3D microfluidic to mimic in vivo returned favorable results that were expected for drug testing in the future.

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