Evaluation of antibiotic sensitivity of potential probiotic Bacillus strains isolated from chicken and pig feces

Hai Van Nguyen; Lan Anh Nguyen; Thi Minh Duc Nguyen; Thi Hanh Nguyen Vu; Quyet Tien Phi; Ha Anh Nguyen; Phu Ha Ho

doi:10.15625/2525-2518/16494

Author affiliations

Authors

Hai Van Nguyen School of Biotechnology and Food Technology, Hanoi Univeristy of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Lan Anh Nguyen School of Biotechnology and Food Technology, Ha Noi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Thi Minh Duc Nguyen School of Biotechnology and Food Technology, Ha Noi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Thi Hanh Nguyen Vu Institute of Biotechnology, Viet Nam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Quyet Tien Phi Institute of Biotechnology, Viet Nam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Ha Anh Nguyen School of Biotechnology and Food Technology, Ha Noi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam
Phu Ha Ho School of Biotechnology and Food Technology, Ha Noi University of Science and Technology, 1 Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16494

Keywords:

Bacillus, probiotic, antibiotic susceptibility, livestock

Abstract

Nowadays, the misuse and overuse of antibiotics in livestock production leads to the appearance of antibiotic resistant strains that promotes the use of probiotics in feed to minimise/replace antibiotic consumption. Among them, Bacilli are common supplements for feed effectiveness and livestock health benefits. However, there is a growing concern about the development of antibiotic resistance and transfer of antibiotic resistance genes among bacteria. Thus, the European Food Safety Authority (EFSA) has suggested that products containing Bacillus strains intended to use as feed additive must be examined for susceptibility to antimicrobials of human and veterinary importance. In this study, the antibiotic susceptibility of potential probiotic Bacillus strains was investigated. A total of 59 Bacillus species were isolated from 10 samples of chicken and pig feces. Then, the in vitro probiotic characteristics including low pH tolerance, catalase activity, protease, amylase and cellulase enzymes production were tested to select appropriate isolates for further studies. Thirty-three promising probiotic strains were assessed for their antibiotic susceptibility against 13 antibiotics by disc diffusion method. In most cases, isolated Bacillus strains were susceptible to tested antibiotics. The obtained results demonstrated the potential application of Bacillus spp. as feed supplements in animal production.

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