Low molecular weight of hydroxyethyl cellulose to extract proteins in natural rubber latex
Author affiliations
DOI:
https://doi.org/10.15625/2525-2518/59/5/15912Keywords:
HEC, natural rubber latex, proteins contentAbstract
Natural rubber latex (NRL) with “low protein content” is regarded as less allergenic latex for medical gloves, medical products, condoms, etc. Therefore, this study was conducted to strengthen the fundamental approach of making “low protein NRL” via oligosaccharide hydroxyl ethyl cellulose (oligoHEC) treatment. OligoHEC (Mw ~10,000 g/mol) is degradation product of HEC (Mw ~90,000 g/mol) by 1.5% H2O2 treatment combined with hydrothermal at temperature for 30 minutes at 121°C, pressurized rate of 0.38 mPa by autoclave equipment. OligoHECs were employed to form electrical bonds with the proteins. The target of this study is protein content of NRL ≤ 50 µg/g of rubber, we surveyed the effect of oligoHEC treatment towards the solution proteins content of NRL. Results show that oligoHEC at low concentration (0.25%) effectively extracted the proteins molecules. Interestingly, allergenic proteins content of NRL serum was decreased proportionally (< 50 µg/g of rubber) with the upsurge of oligoHEC concentration, suggesting deactivation of allergenic. These preliminary results indicate a potential approach to produce low allergenic risk NRL products with adding of oligoHEC
Downloads
References
Tomazic-Jezic V. J., Lucas A. D. - Protein and allergen assays for natural rubber latex products, J. Allergy. Clin. Immunol. 110 (2002) 6. DOI: 10.1067/mai.2002.125335 DOI: https://doi.org/10.1067/mai.2002.125335
Cornish K. - Assessment of the risk of Type I latex allergy sensitization or reaction during use of products made from latex derived from guayule and other alternate rubber-producing species, Rubb. Sci. 25(2) (2012) 139. http://www.rubberscience.in/download.php?id=cfk6qhnb6t3arjjfe20dl6lir72.
Srisatjang J. Duangkamol D., Panu D. - Extraction of protein from skim natural rubber latex by continuous flow, 1st Mae Fah Luang U. Inter. Conf. (2012) 1.
Yamamoto Y., Nghia P. T., Klinklai W., Saito T., and Kawahara S. - Removal of proteins from natural rubber with urea and its application to continuous processes, J. Appl. Polym. Sci. 107 (2008) 2329. https://doi.org/10.1002/app.27236 DOI: https://doi.org/10.1002/app.27236
Andler R., Steinbüchel A. - A simple, rapid and cost-effective process for production of latex clearing protein to produce oligopolyisoprene molecules, J. Biotechnol. 241 (2017) 184. DOI: 10.1016/j.jbiotec.2016.12.008 DOI: https://doi.org/10.1016/j.jbiotec.2016.12.008
Yeang H. Y., Sunderasan E. and Ghazali H. M. - Latex Allergy Studies: Extraction of natural rubber latex proteins with reference to film thickness, latex d.r.c. and protein migration behaviour, J. Nat. Rubb. Res. 10(1) (1995) 6.
Fukuhara L., Miyano K., Yamamoto Y., Ishii H. and Kawahara S. - Preparation of purified natural rubber by removal of proteins, Kobunshi Ronbunshu 72(1)•(2015), 1. DOI: 10.1295/koron.2014-0058. DOI: https://doi.org/10.1295/koron.2014-0058
Tangboriboon N., Phudkrachang P., Mulsow L., Kunchornsup W., and Sirivat A. - Removal of water extractable proteins from concentrated natural rubber latex by eggshells, J. Elastomers. Plast. 45(3) (2012) 253. https://doi.org/10.1177/0095244312452273. DOI: https://doi.org/10.1177/0095244312452273
Hassan A. A., Rahman N. A., Abdullah N., Sajari R., Lang M. K. - Application of proteolytic enzyme in high ammoniated natural rubber latex, Malaysian J. Anal. Sci. 22(2) (2018) 286. DOI: 10.17576/mjas-2018-2202-14. DOI: https://doi.org/10.17576/mjas-2018-2202-14
Abhilash G., Sabharwal S., Dubey A., Paul J., John H., Joseph R. - Preparation of low-protein natural rubber latex: Effect of polyethylene glycol, J. Appl. Polym. Sci. 114 (2009). https://doi.org/10.1002/app.30491. DOI: https://doi.org/10.1002/app.30491
Yamamoto Y., Norulhuda S. N. B., Nghia P. T., Kawahara S. - Thermal degradation of deproteinized natural rubber, Polym. Degrad. Stabil. 156 (2019) 144. https://doi.org/10.1016/j.polymdegradstab.2018.08.003. DOI: https://doi.org/10.1016/j.polymdegradstab.2018.08.003
Mahendra I P., Linh M. K., Thang N. N., Thuy V. T., Trang L. T., Thinh L. X., Phuong N.T. H., Ha N. T., Thuong N. T., Kawahara S., Yamamoto Y., and Nghia P. T. - Protein removal from natural rubber latex with Fe3O4@Al2O3 nanoparticle, J. Braz. Chem. Soc. 32(2) (2021) 320. https://dx.doi.org/10.21577/0103-5053.20200182 DOI: https://doi.org/10.21577/0103-5053.20200182
Danwanichakul D., Rattanaphan O., Srisatjang J., Danwanichakul P. - Extraction of protein from skim natural rubber latex using PEG as a surfactant via low speed centrifugation and continuous flow, Inc. J. Appl. Polym. Sci. 131 (2014) 39900. https://doi.org/10.1002/app.39900. DOI: https://doi.org/10.1002/app.39900
Duclerc F. P., Carlos F. P. Martins, Chirinos H. D. C., Ademar B.L. - Extractable proteins from field radiation vulcanized natural rubber latex, Nucl. Instrum. Methods Phys. Res., B: Beam Interac. Mater. Ato. 236(1-4) (2005) 508. https://doi.org/10.1016/j.nimb.2005.04.028. DOI: https://doi.org/10.1016/j.nimb.2005.04.028
ASTM D5712–10, Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method
Quin C. Q., Du Y. M., Xiao L., - Effect of hydrogen peroxide treatment on the molecular weight and structure of chitosan, Polym. Degrad. Stab. 76 (2002) 211. DOI: 10.1016/S0141-3910(02)00016-2. DOI: https://doi.org/10.1016/S0141-3910(02)00016-2
Von Sonntag C. - Free-radical reaction of carbohydrates as studied by radiation techniques, Adv. Carbohydr. Chem. Biochem. 50 (1980) 7. https://doi.org/10.1016/S0065-2318(08)60019-0. DOI: https://doi.org/10.1016/S0065-2318(08)60019-0
Mekonnen T. H., Ah-Leung T., Hojabr S., Berry R. - Investigation of the co-coagulation of natural rubber latex and cellulose nanocrystals aqueous dispersion, Colloids Surf. A 583 (2019) 123949. https://doi.org/10.1016/j.colsurfa.2019.123949. DOI: https://doi.org/10.1016/j.colsurfa.2019.123949
Crippa M., Belleri L., Mistrello G., Tedoldi C., Alessio L., - Prevention of latex allergy among health care workers and in the general population: latex protein content in devices commonly used in hospitals and general practice, Int. Arch. Occup. Environ. Health. 79(7) (2006) 550. DOI: 10.1007/s00420-005-0080-5. DOI: https://doi.org/10.1007/s00420-005-0080-5
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Vietnam Journal of Sciences and Technology (VJST) is an open access and peer-reviewed journal. All academic publications could be made free to read and downloaded for everyone. In addition, articles are published under term of the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA) Licence which permits use, distribution and reproduction in any medium, provided the original work is properly cited & ShareAlike terms followed.
Copyright on any research article published in VJST is retained by the respective author(s), without restrictions. Authors grant VAST Journals System a license to publish the article and identify itself as the original publisher. Upon author(s) by giving permission to VJST either via VJST journal portal or other channel to publish their research work in VJST agrees to all the terms and conditions of https://creativecommons.org/licenses/by-sa/4.0/ License and terms & condition set by VJST.
Authors have the responsibility of to secure all necessary copyright permissions for the use of 3rd-party materials in their manuscript.
Vietnam Journal of Science and Technology (VJST) is pleased to notice: