Extraction and physico-chemical characterization of water-soluble polysaccharides from Opuntia dillenii (Ker Gawl.) Haw. Cladodes

Thi-Hong-An Nguyen; Hoang Thi Kim Dung; Thi-Thuy-Huong Nguyen

doi:10.15625/2525-2518/59/2/15510

Author affiliations

Authors

Thi-Hong-An Nguyen Institute of Chemical Technology, VAST, 1 Mac Dinh Chi, Dist.1, Ho Chi Minh City, Viet Nam
Hoang Thi Kim Dung Institute of Chemical Technology, VAST, 1 Mac Dinh Chi, Dist.1, Ho Chi Minh City, Viet Nam https://orcid.org/0000-0002-9369-8051
Thi-Thuy-Huong Nguyen Ton Duc Thang University, 19 Nguyen Huu Tho, Dist. 7, Ho Chi Minh City, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/59/2/15510

Keywords:

Opuntia dillenii (Ker Gawl.) Haw., Cactaceae, mucilage, polysaccharide, water-soluble polysaccharide

Abstract

Opuntia dillenii (Ker Gawl.) Haw., a well-known member of the Cactaceae family, played an important role in agriculture and medicine. In this study, the extraction conditions and the physico-chemical properties of water-soluble polysaccharides (OMP) from Opuntia dillenii mucilage were investigated. The cactus cladodes used in the study were collected from Binh Thuan province, Vietnam. The optimum extraction conditions of water extraction with conventional heating included at temperature 70^oC in 120 min. The crude and deproteinized polysaccharides yield were 17.7 % and 7.9 %. By analysis of gel permeation chromatography (GPC), the study showed that the polysaccharides possessed a low average molecular weight (129,681 Da). In addition, the characterization of polysaccharide was determined by Fourier-Transform Infrared Spectroscopy (FTIR), and High-Performance Liquid Chromatography (HPLC). The results indicated that the types of sugars in the water-soluble polysaccharides extracted.

Downloads

Download data is not yet available.

References

U. U. Shedbalkar, V. S. Adki, J. P. Jadhav and V. A. Bapat. -Opuntia and other cacti: applications and biotechnological insights, 3 (2010) 136-150. doi:10.1007/s12042-010-9055-0 DOI: https://doi.org/10.1007/s12042-010-9055-0

R. Shirazinia, V. B. Rahimi, A. R. Kehkhaie, A. Sahebkar, H. Rakhshandeh and V. R. Askari. -Opuntia dillenii: A Forgotten Plant with Promising Pharmacological Properties, 22 (2019) 16. doi: 10.3831/KPI.2019.22.002 DOI: https://doi.org/10.3831/KPI.2019.22.002

H. Li, Q. Yuan, X. Zhou, F. Zeng and X. Lu. -Extraction of Opuntia dillenii Haw. polysaccharides and their antioxidant activities, 21 (2016) 1612. https://doi.org/10.3390/molecules21121612 DOI: https://doi.org/10.3390/molecules21121612

L. Zhao, Q. Lan, Z. Huang, L. Ouyang and F. Zeng. -Antidiabetic effect of a newly identified component of Opuntia dillenii polysaccharides, 18 (2011) 661-668. doi:10.1016/j.phymed.2011.01.001 DOI: https://doi.org/10.1016/j.phymed.2011.01.001

L.-Y. Zhao, W. Huang, Q.-X. Yuan, J. Cheng, Z.-C. Huang, L.-J. Ouyang and F.-H. Zeng. -Hypolipidaemic effects and mechanisms of the main component of Opuntia dillenii Haw. polysaccharides in high-fat emulsion-induced hyperlipidaemic rats, 134 (2012) 964-971. doi:10.1016/j.foodchem.2012.03.001 DOI: https://doi.org/10.1016/j.foodchem.2012.03.001

P. Kalegowda, A. S. Chauhan and S. M. N. Urs. -Opuntia dillenii (Ker-Gawl) Haw cladode mucilage: Physico-chemical, rheological and functional behavior, 157 (2017) 1057-1064. doi:10.1016/j.carbpol.2016.10.070 DOI: https://doi.org/10.1016/j.carbpol.2016.10.070

X.-K. Zhong, X. Jin, F.-Y. Lai, Q.-S. Lin and J.-G. Jiang. -Chemical analysis and antioxidant activities in vitro of polysaccharide extracted from Opuntia ficus indica Mill. cultivated in China, 82 (2010) 722-727. doi:10.1016/j.carbpol.2010.05.042 DOI: https://doi.org/10.1016/j.carbpol.2010.05.042

R. B. Lima, T. B. dos Santos, L. G. E. Vieira, M. d. L. L. Ferrarese, O. Ferrarese-Filho, L. Donatti, M. R. T. Boeger and C. L. de Oliveira Petkowicz. -Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.), 93 (2013) 135-143. doi:10.1016/j.carbpol.2012.05.015 DOI: https://doi.org/10.1016/j.carbpol.2012.05.015

D. Yang and H. Yang. -The temperature dependent extraction of polysaccharides from eucheuma and the rheological synergistic effect in their mixtures with kappa carrageenan, (2020) 109515. doi:10.1016/j.lwt.2020.109515 DOI: https://doi.org/10.1016/j.lwt.2020.109515

C. Jiang, M. Wang, J. Liu, D. Gan and X. Zeng. -Extraction, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclina sinensis, 84 (2011) 851-857. doi:10.1016/j.carbpol.2010.11.027 DOI: https://doi.org/10.1016/j.carbpol.2010.11.027

Q. Shao, Y. Deng, H. Fang and X. Zhao. -Optimization of polysaccharides extraction from Tetrastigma hemsleyanum Diels et Gilg using response surface methodology, 49 (2011) 958-962. doi:10.1016/j.ijbiomac.2011.08.015 DOI: https://doi.org/10.1016/j.ijbiomac.2011.08.015

M. Monrroy, E. García, K. Ríos and J. R. García. -Extraction and physicochemical characterization of mucilage from Opuntia cochenillifera (L.) Miller, 2017 (2017). https://doi.org/10.1155/2017/4301901 DOI: https://doi.org/10.1155/2017/4301901

G. Ginestra, M. L. Parker, R. N. Bennett, J. Robertson, G. Mandalari, A. Narbad, R. B. Lo Curto, G. Bisignano, C. B. Faulds and K. W. Waldron. -Anatomical, chemical, and biochemical characterization of cladodes from prickly pear [Opuntia ficus-indica (L.) Mill.], 57 (2009) 10323-10330. https://doi.org/10.1021/jf9022096 DOI: https://doi.org/10.1021/jf9022096

M. Espino-Díaz, J. De Jesús Ornelas-Paz, M. A. Martínez-Téllez, C. Santillán, G. V. Barbosa-Cánovas, P. B. Zamudio-Flores and G. I. Olivas. -Development and characterization of edible films based on mucilage of Opuntia ficus-indica (L.), 75 (2010) E347-E352. https://doi.org/10.1111/j.1750-3841.2010.01661.x DOI: https://doi.org/10.1111/j.1750-3841.2010.01661.x

TCVN: 10324:2017 DOI: https://doi.org/10.5465/AMBPP.2017.10324abstract