OPTIMIZATION AND PURIFICATION OF α-GLUCOSIDASE INHIBITOR FROM BACILLUS SUBTILIS YT20 ISOLATED IN VIETNAM
Keywords:B. subtilis YT20, chromatography, 1-deoxynojirimicin, α-glucosidase inhibitor
Among the treatments of type 2 diabetes, the α-glucosidase inhibitor has been drawing the researcher’s interest world-wide because of safe and convenient mechanism. However, recent α-glucosidase inhibitors have side effects, so alternative sources have been studied to limit the side effects and provide more options for the treatment of type 2 diabetes. In this study, after selecting morioorganism strains capable of biosynthesis of α-glucosidase inhibitor from the collection of Bacillus subtilis, we have isolated B. subtilis YT20 which inhibited α-glucosidase above 90%. In detail, this number increased multiply by 1.4 times after the optimization of culture conditions. The α-glucosidase inhibitor activity of B. subtilis YT20 yield the molecular weight of 163 Da after purified by a combination of salting out, filter membrane with pore size 1000 Da and 100 Da respectively, activated charcoal and chromatography column Sephadex G100, the efficiency peak at 16%. The spectral data indicated that the putative compound isolated from B. subtilis YT20 was 1-deoxynojirimicin. In the thin layer chromatography result, there was an appearance of a single band which corresponds to standard 1-deoxynojirimicin (Sigma), followed by a retention factor of 0.34 with a solvent system of isopropanol: acetic acid: deionized water (4:1:1).
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