STUDY ON MAKING TYROSINASE BIOSENSOR FROM ASPERGILLUS ORYZAE TP01 AND ITS APPLICATION
Tyrosinase (EC 188.8.131.52) is a Polyphenol oxidase. It is a bifunctional enzyme catalyzing the monohydroxylation of monophenols and the oxidation of diphenols. Tyrosinase is used to medicine, food industry and environmental protection, especially used to create the biosensor.
Tyrosinase has been purified by Mono Q HR 5/5 column in FPLC system with the result of 18.96 folds as the rude enzym, its specific activity is 204.75 U/mgPr and its yield is 31.63%.
Determining the optimum condition to immobilised the enzym on biosensor: the amout of tyrosinase is 50U, duration of 30 minutes, BSA of 1.3mg in saturated glutaraldehyt, reutilization of 12 times. After 50 days the biosensor is preserved in the tinned bag at 4oC, the output signal remains 79.51%.
The optimum condition of the biosensor is the temperature of 30oC, pH 6.6, subtrate density of 10-7 – 4 × 10-4 M. Biosensor has been detected monophenol compound from the density of 10-7 M–3 × 10-4 M and diphenol compound from 10-6 M – 4 × 10-4 M.
Application of tyrosinase biosensor has been detected atrazin, diuron, 2.4D and phenol compounds in waste water from the petrolium, chemical, tobacco, dying and soaping factories.
Authors who publish with Vietnam Journal of Science and Technology agree with the following terms:
- The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
- The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).