STUDY THE ANTICANCER MECHANISM OF THE PROMISSING COMPOUND 2B2D BY USING MICROARRAY TECHNIQUE
Keywords:cDNA, cyanine, LU-1, Mallotus apelta, microarray, spectrophotometer
AbstractBeing a modern technique with the ability of studying, discovering, probing and analyzing the expression of thousands genes, even the whole genome in the only one experiment, microarray proved to be a powerful tool for cancer research especially at molecular level. Employing the potential anticancer compound 1-(5,7-dimetoxy-2,2-dimetyl-2H-cromen-8-yl)-but-2-en-1-on (2B2D in short) and LU-1, the human lung cancer cells as research objects, we successfully hybridized the cy3/5 incorporated cDNA with Phalanx HOV5 microarray. The results showed 742 genes that got effected with equal or over two folds change under 2B2D treatment. Among - those, 386 genes were up-regulated while the other 356 were down-regulated. The Nuclear factor (erythroid-derived 2, regulatory factor X domain containing 1, fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) and E2F transcription factor 8 genes were the most stimulated by our compound. The genes that named as Solute carrier family 7 (cationic amino acid transporter, system) member 11, kelch-like 24 and Hypothetical LOC344887 were the most down in action.
Authors who publish with Vietnam Journal of Science and Technology agree with the following terms:
- The manuscript is not under consideration for publication elsewhere. When a manuscript is accepted for publication, the author agrees to automatic transfer of the copyright to the editorial office.
- The manuscript should not be published elsewhere in any language without the consent of the copyright holders. Authors have the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of their work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are encouraged to post their work online (e.g., in institutional repositories or on their websites) prior to or during the submission process, as it can lead to productive exchanges or/and greater number of citation to the to-be-published work (See The Effect of Open Access).