Study the anticancer mechanism of the promissing compound 2B2D by using microarray technique

Authors

  • Thao Thi Do Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam
  • Phuong Thi Do Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam
  • Trang Thi Nguyen Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam
  • Nga Thi Nguyen Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Viet Nam
  • Ying Chi Huang Clinical Medicine Research Institute, The National Yang-Ming University, Taiwan

DOI:

https://doi.org/10.15625/0866-708X/50/3/9496

Keywords:

cDNA, cyanine, LU-1, Mallotus apelta, microarray, spectrophotometer

Abstract

Being a modern technique with the ability of studying, discovering, probing and analyzing the  expression  of  thousands  genes,  even  the  whole  genome  in  the  only  one  experiment, microarray  proved  to  be  a  powerful  tool  for  cancer  research  especially  at  molecular  level. Employing  the  potential  anticancer  compound  1-(5,7-dimetoxy-2,2-dimetyl-2H-cromen-8-yl)-but-2-en-1-on  (2B2D  in  short) and LU-1,  the human  lung cancer cells as  research objects, we successfully  hybridized  the  cy3/5  incorporated  cDNA  with  Phalanx  HOV5  microarray.  The results  showed  742  genes  that  got  effected with  equal  or  over  two  folds  change  under  2B2D treatment.  Among  -  those,  386  genes  were  up-regulated  while  the  other  356  were  down-regulated. The Nuclear  factor  (erythroid-derived  2,  regulatory  factor X  domain  containing  1, fibroblast  growth  factor  receptor  3  (achondroplasia,  thanatophoric  dwarfism)  and  E2F transcription factor 8 genes were the most stimulated by our compound. The genes that named as Solute carrier  family 7  (cationic amino acid  transporter,  system) member 11,  kelch-like 24 and Hypothetical LOC344887 were the most down in action.

Downloads

Download data is not yet available.

Published

2017-04-06

How to Cite

[1]
T. T. Do, P. T. Do, T. T. Nguyen, N. T. Nguyen, and Y. C. Huang, “Study the anticancer mechanism of the promissing compound 2B2D by using microarray technique”, Vietnam J. Sci. Technol., vol. 50, no. 3, pp. 267–274, Apr. 2017.

Issue

Section

Articles