Optimization of enzymatic hydrolysis conditions for increasing the efficiency of dry matter extracted from limonia acidissima pulp by combined cellulase -pectinase enzymes using response surface methodology

Authors

  • Pham Bao Nguyen Postharvest Technology center, Tra Vinh University, 126 Nguyen Thien Thanh street, Ward 5, Tra Vinh city, Vietnam
  • Dong Thi Anh Dao Dept. Food Technology, Vietnam Nat. Uni. HCM University of Technology, 268 Ly Thuong Kiet Street, Ward 14, District 10, HCMC, Vietnam

DOI:

https://doi.org/10.15625/0866-708X/55/1/7472

Keywords:

Optimization, cellulase, pectinase, Limonia acidissima, DPPH, ABTS radical scavenging activity

Abstract

The Limonia acidissima (L. acidissima) fruit is rich nutrient and bioactive compounds. Our research on the hydrolysis of L. acidissima pulp by combined cellulase and pectinase enzymes to increase the yield of dry matter and bioactive compounds for health benefit is important. In this study, the hydrolysis conditions by combined enzymes for the dry matter recovery were optimized by response surface methodology (RSM). The independent variables were coded pH (X1), incubation temperature (X2), the total content of combined cellulase-pectinase (X3) (with the ratio of cellulase/pectinase was 1/1), and hydrolysis time (X4). The results of the analysis of variance (ANOVA) showed that the variables actively affected the efficiency of extracted dry matter. The optimal conditions of hydrolysis were derived at x1 = 4.2, x2 = 45 oC, x3 = 1.6 % (v/dwt), x4 = 120minutes, respectively in the efficiency of the prediction model was 54.76 %. that increased 20.89 % in compared with non-enzymatic extraction. Besides, the recovery efficiency of carbohydrates was 87.74 %. Further, all the content of extracted phenolic content (TPC), carotenoic and DPPH and ABTS radical scavenging activity highly increased. That reached 106.7mg GAE, 86.6mg, 67.1 and 102.1 mg trolox equivalent antioxidant capacity (TEAC) from 100g L. acidissima pulp respectively.

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Author Biography

Pham Bao Nguyen, Postharvest Technology center, Tra Vinh University, 126 Nguyen Thien Thanh street, Ward 5, Tra Vinh city, Vietnam

Giảng viên TT Công nghệ sau thu hoạch - khoa Nông Nghiệp -Thủy Sản - trường Đại học Trà Vinh

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Published

2017-02-09

How to Cite

[1]
P. B. Nguyen and D. T. A. Dao, “Optimization of enzymatic hydrolysis conditions for increasing the efficiency of dry matter extracted from limonia acidissima pulp by combined cellulase -pectinase enzymes using response surface methodology”, Vietnam J. Sci. Technol., vol. 55, no. 1, pp. 15–28, Feb. 2017.

Issue

Section

Natural Products