EXTRACTION AND ISOLATION OF COLLAGEN FROM THE SKINS OF BASA FISH (PANGASIUS HYPOPHTHALMUS)

Phan Dinh Tuan, Le Thi Thu Huong, Nguyen Hoang Dzung
Author affiliations

Authors

  • Phan Dinh Tuan
  • Le Thi Thu Huong Trường Đại học Bách khoa TP. HCM
  • Nguyen Hoang Dzung Trường Đại học Bách khoa TP. HCM

DOI:

https://doi.org/10.15625/0866-708X/52/4/3218

Abstract

Acid-solubilized collagen (ASC) was extracted from basa fish skin (Pangasius hypophthalmus) by acetic acid and purified by dialysis, lyophilization and super-critical (SC) CO2 extraction techniques. Dialysis yield for salt removal reached 93.3% in stirring condition at 24h, while the efficiency of lipid extraction process could reach 91.6% at conditions: Pressure- 200 bar, CO2 flow rate of 10g/min, temperature of 38oC, concentration of ethanol as co-solvent of 10% (v/v) and the retention time of 60 minutes. The obtained collagen has low impurities of lipid (0.33%), ashes (0.29%) and apprears to be of no smell, no color, high molecular weight, which is appropriate for cosmetic, pharmaceutical and food application.

Keywords: basa fish, super critical CO2, Pangasius hypophthalmus

Downloads

Download data is not yet available.

Downloads

Published

24-08-2014

How to Cite

[1]
P. D. Tuan, L. T. Thu Huong, and N. H. Dzung, “EXTRACTION AND ISOLATION OF COLLAGEN FROM THE SKINS OF BASA FISH (PANGASIUS HYPOPHTHALMUS)”, Vietnam J. Sci. Technol., vol. 52, no. 4, pp. 431–440, Aug. 2014.

Issue

Section

Articles