Potent inhibitory effect on human platelet aggregation of the aerial part of Canna edulis
Author affiliations
DOI:
https://doi.org/10.15625/2525-2518/19013Keywords:
Antiplatelet, Canna edulis, cardiovascular, agonistAbstract
Canna edulis Ker Gawl has been used in Vietnamese Traditional Medicine for the treatment of heart diseases with very limited scientific evidence. This study aimed to evaluate the inhibitory effect on human platelet aggregation of the aerial part of C. edulis. Aerial C. edulis was firstly macerated with methanol, and then fractionated with different solvents to obtain 3 fractions: n-hexane, ethyl acetate and aqueous extracts. The inhibitory effects on human platelet aggregation of extracts were evaluated via three parameters: percentage inhibition of platelet aggregation (%I), area under the aggregation curve (AUC) and aggregation slope, using two agonists adenosine diphosphate (ADP) and collagen. The results showed that all extracts significantly inhibited platelet aggregation in a dose-dependent manner for both agonists. Moreover, all extracts significantly decreased AUC and slope, indicating their antiaggregatory effects on both general aggregation and aggregation velocity. Among extracts, the ethyl acetate fraction exhibited the strongest effect regardless of agonists used (%I at 4 mg/mL, 99.2% for both ADP and collagen). The n-hexane fraction also had significant inhibitory effect but it was weaker than the others (p < 0.05). This is the first study to demonstrate the potent antiplatelet effect of the aerial part of C. edulis. This plant could be a potential natural source for searching novel antiplatelet agents and developing dietary supplements for management of cardiovascular diseases.
Downloads
References
Versteeg H. H., Heemskerk J. W., Levi M., Reitsma P. H. - New fundamentals in hemostasis. Physiol Rev, 93 (2013) 327-358. doi.org/10.1152/physrev.00016.2011. DOI: https://doi.org/10.1152/physrev.00016.2011
Wendelboe A. M., Raskob G. E. - Global burden of thrombosis: epidemiologic aspects. Circ Res, 118 (2016) 1340-1347. doi.org/10.1161/CIRCRESAHA.115.306841. DOI: https://doi.org/10.1161/CIRCRESAHA.115.306841
Cattaneo M. - Aspirin and clopidogrel: efficacy, safety, and the issue of drug resistance. Arterioscler Thromb Vasc Biol, 24 (2004) 1980-1987. doi.org/10.1161/01.ATV.0000145980.39477.a9. DOI: https://doi.org/10.1161/01.ATV.0000145980.39477.a9
Chan N. C., Weitz J. I. - Antithrombotic agents. Circ. Res., 124 (2019) 426-436. doi.org/10.1161/CIRCRESAHA.118.313155. DOI: https://doi.org/10.1161/CIRCRESAHA.118.313155
Le H. L., Nguyen T. M. H., Vu T. T., Nguyen T. T. O., Ly H. D. T., Le N. T., Nguyen V. H., Nguyen T. V. A. - Potent antiplatelet aggregation, anticoagulant and antioxidant activity of aerial Canna x generalis L.H Bailey & E.Z Bailey and its phytoconstituents. S. Afr. J. Bot., 147 (2022) 882-893. doi.org/10.1016/j.sajb.2022.03.035. DOI: https://doi.org/10.1016/j.sajb.2022.03.035
Nguyen T. M. H., Le H. L., Ha T. T., Bui B. H., Le N. T., Nguyen V. H., Nguyen T. V. A. - Inhibitory effect on human platelet aggregation and coagulation and antioxidant activity of C. edulis Ker Gawl rhizome and its secondary metabolites. J. Ethnopharmacol., 263 (2020) 113136. doi.org/10.1016/j.jep.2020.113136. DOI: https://doi.org/10.1016/j.jep.2020.113136
Vu T., Le Q. - Edible Canna (Canna edulis Ker), A potential crop for Vietnam food industry. Int J Botany Stud, 4 (2019) 58-59.
Mishra T., Goyal A. K., Middha S., Sen A. - Antioxidative properties of Canna edulis Ker-Gawl. Indian J Nat Prod Resour, 2 (2011) 315-321.
Zhang J., Wang Z. W., Mi Q. - Phenolic compounds from Canna edulis Ker residue and their antioxidant activity. LWT - Food Science and Technology, 44 (2011) 2091-2096. doi.org/10.1016/j.lwt.2011.05.021. DOI: https://doi.org/10.1016/j.lwt.2011.05.021
Xie F., Gong S., Zhang W., Wu J., Wang Z. - Potential of lignin from Canna edulis ker residue in the inhibition of α-d-glucosidase: kinetics and interaction mechanism merging with docking simulation. Int J Biol Macromol, 95 (2017) 592-602. doi.org/10.1016/j.ijbiomac.2016.11.100. DOI: https://doi.org/10.1016/j.ijbiomac.2016.11.100
Xie F., Zhang W., Gong S., Gu X., Lan X., Wu J., Wang Z. - Investigating lignin from Canna edulis ker residues induced activation of α-amylase: kinetics, interaction, and molecular docking. Food Chem, 271 (2019) 62-69. doi.org/10.1016/j.foodchem.2018.07.153. DOI: https://doi.org/10.1016/j.foodchem.2018.07.153
Xie F., Zhang W., Gong S., Wang Z. - Inhibitory effect of lignin from Canna edulis Ker residues on trypsin: kinetics and molecular docking studies. J Sci Food Agric, 101 (2021) 2090-2099. doi.org/10.1002/jsfa.10831. DOI: https://doi.org/10.1002/jsfa.10831
Mustard J. F., Perry D. W., Ardlie N. G., Packham M. A. - Preparation of suspensions of washed platelets from humans. Br. J. Haematol., 22 (1972) 193-204. doi.org/10.1111/j.1365-2141.1972.tb08800.x. DOI: https://doi.org/10.1111/j.1365-2141.1972.tb08800.x
Le Blanc J., Mullier F., Vayne C., Lordkipanidzé M. - Advances in platelet function testing-light transmission aggregometry and beyond. J Clin Med, 9 (2020). doi.org/10.3390/jcm9082636. DOI: https://doi.org/10.3390/jcm9082636
Hanke A. A., Roberg K., Monaca E., Sellmann T., Weber C. F., Rahe-Meyer N., Görlinger K. - Impact of platelet count on results obtained from multiple electrode platelet aggregometry (Multiplate). Eur. J. Med. Res., 15 (2010) 214-219. doi.org/10.1186/2047-783x-15-5-214. DOI: https://doi.org/10.1186/2047-783X-15-5-214
Cattaneo M. - ADP receptors: inhibitory strategies for antiplatelet therapy. Drug News Perspect, 19 (2006) 253-259. doi.org/10.1358/dnp.2006.19.5.985936. DOI: https://doi.org/10.1358/dnp.2006.19.5.985936
Farndale R. W. - Collagen-induced platelet activation. Blood Cells Mol Dis, 36 (2006) 162-165. doi.org/10.1016/j.bcmd.2005.12.016. DOI: https://doi.org/10.1016/j.bcmd.2005.12.016
Le H. L., Vu T. T., Le T. T. H., Duong T. L. H., Nguyen T. V. A. - Inhibitory effect on human platelet aggregation, antioxidant activity, and phytochemicals of Canna warszewiczii (A. Dietr) Nb. Tanaka. Pharmacogn. Res., 12 (2020) 47-52. DOI: https://doi.org/10.4103/pr.pr_72_19
Assefa A. D., Ko E. Y., Moon S. H., Keum Y. S. - Antioxidant and antiplatelet activities of flavonoid-rich fractions of three citrus fruits from Korea. 3 Biotech, 6 (2016) 109. doi.org/10.1007/s13205-016-0424-8. DOI: https://doi.org/10.1007/s13205-016-0424-8
Srivastava K. C. - Effects of aqueous extracts of onion, garlic and ginger on platelet aggregation and metabolism of arachidonic acid in the blood vascular system: in vitro study. Prostaglandins Leukot Med, 13 (1984) 227-235. DOI: https://doi.org/10.1016/0262-1746(84)90014-3
Liao Y. R., Leu Y. L., Chan Y. Y., Kuo P. C., Wu T. S. - Anti-platelet aggregation and vasorelaxing effects of the constituents of the rhizomes of Zingiber officinale. Molecules, 17 (2012) 8928-8937. doi.org/10.3390/molecules17088928. DOI: https://doi.org/10.3390/molecules17088928
Son Y. M., Jeong D. H., Park H. J., Rhee M. H. - The inhibitory activity of ginsenoside Rp4 in adenosine diphosphate-induced platelet aggregation. J Ginseng Res, 41 (2017) 96-102. doi.org/10.1016/j.jgr.2016.01.003. DOI: https://doi.org/10.1016/j.jgr.2016.01.003
Shin J. H., Kwon H. W., Irfan M., Rhee M. H., Lee D. H. - Ginsenoside Rk1 suppresses platelet mediated thrombus formation by downregulation of granule release and α(IIb)β(3) activation. J Ginseng Res, 45 (2021) 490-497. doi.org/10.1016/j.jgr.2020.11.001. DOI: https://doi.org/10.1016/j.jgr.2020.11.001
Santhakumar A. B., Bulmer A. C., Singh I. - A review of the mechanisms and effectiveness of dietary polyphenols in reducing oxidative stress and thrombotic risk. J Hum Nutr Diet, 27 (2014) 1-21. doi.org/10.1111/jhn.12177. DOI: https://doi.org/10.1111/jhn.12177
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Vietnam Journal of Sciences and Technology (VJST) is an open access and peer-reviewed journal. All academic publications could be made free to read and downloaded for everyone. In addition, articles are published under term of the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA) Licence which permits use, distribution and reproduction in any medium, provided the original work is properly cited & ShareAlike terms followed.
Copyright on any research article published in VJST is retained by the respective author(s), without restrictions. Authors grant VAST Journals System a license to publish the article and identify itself as the original publisher. Upon author(s) by giving permission to VJST either via VJST journal portal or other channel to publish their research work in VJST agrees to all the terms and conditions of https://creativecommons.org/licenses/by-sa/4.0/ License and terms & condition set by VJST.
Authors have the responsibility of to secure all necessary copyright permissions for the use of 3rd-party materials in their manuscript.
Vietnam Journal of Science and Technology (VJST) is pleased to notice: