Chemical composition, antioxidant activity and extraction optimization of Distichochlamys orlowii rhizomes

Le Hong Luyen; Nguyen Thi Linh; Le Thi  Hong

doi:10.15625/2525-2518/18666

Author affiliations

Authors

Le Hong Luyen Department of Life Sciences, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Thi Linh Department of Life Sciences, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0009-0009-5701-1046
Le Thi Hong 2University of Medicine and Pharmacy, Vietnam National University of Hanoi, 144 Xuan Thuy, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/18666

Keywords:

Distichochlamys orlowii, volatile components, fatty acids, amino acids, antioxidant capacity

Abstract

Distichochlamys orlowii is an endemic ginger species in Vietnam. The present study aimed to investigate the chemical composition, antioxidative capacity and extraction optimization of D. orlowii rhizomes (DO-R) for the first time. The results demonstrated that DO-R possessed more unsaturated fatty acids (53.10%) than saturated fatty acids (46.90%). Many unsaturated fatty acids of great health benefits were present, including trans-13-octadecenoic acid (C18:1), linoleic acid (C18:2), arachidonic acid (C20:4) and eicosapentaenoic acid (C20:5). In addition, seven essential amino acids (EAA) were detected with 41.79 ± 1.02 mg. In comparison, ten non-essential amino acids (N-EAA) were found at a much higher amount of 252.09 ± 1.06 mg in 100 g DO-R. Valine and arginine were the most abundant EAA and N-EAA in DO-R. The methanol fraction from DO-R was shown to have a higher total phenolic content (TPC = 28.85 ± 0.74 mg GAE/g DW) and total flavonoid content (TFC = 14.03 ± 0.31 mg QE/g DW) than other fractions. In addition, this extract was also the most effective antioxidative agent with an IC₅₀ value of 168.63 ± 4.83 and 153.80 ± 8.24 µg/mL against DPPH and ABTS radical, respectively. The highest extraction yield of DO-R in methanol (95.534 ± 0.593 mg/g DW) was achieved using response surface methodology with a Box-Behnken design under the optimal parameters: a solid-liquid ratio of 1:40 g/mL, an ultrasonic power level of 80%, an extraction time of 60 min, and an extraction temperature of 58°C. In conclusion, D. orlowii can serve as a source of high-potential compounds for further pharmacological applications.

Downloads

Download data is not yet available.

References

El-Ghorab A. H., Nauman M., Anjum F. M., Hussain S., and Nadeem M. A. - Comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and cumin (Cuminum cyminum), J. Agric. Food Chem. 58 (2010) 8231-8237. https://doi.org/10.1021/jf101202x.

Beristain-Bauza S. D. C., Hernández-Carranza P., Cid-Pérez T. S., Ávila-Sosa R., Ruiz-López I. I., Ochoa-Velasco C. E. - Antimicrobial activity of ginger (Zingiber officinale) and its application in food products, Food Rev. Int. 35 (2019) 407-426. https://doi.org/10.1080/87559129.2019.1573829.

Gurung A., Khatiwada B., Kayastha B., Parsekar S., Mistry S. K., Yadav U. N. - Effectiveness of Zingiber officinale (ginger) compared with non-steroidal anti-inflammatory drugs and complementary therapy in primary dysmenorrhoea: A systematic review, Clin. Epidemiology Glob. Health 18 (2022) 101152. ttps://doi.org/ 10.1016/j.cegh.2022.101152.

Liao D. W., Cheng C., Liu J., Zhao L., Huang D.-C., Chen G. T. - Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods, Int. J. Biol Macromol. 152 (2020) 894-903. 10.1016/j.ijbiomac.2020.02.325.

Newman M. F. - Distichochlamys, a new genus from Vietnam, Edinburgh J. Botany 52 (1995) 65-69. https://doi.org/10.1017/S096042860000192X.

KaiLarsen and MarkNewman - A new species of Distichochlamys from Vietnam and some observations on generic limits in Hedychieae (Zingiberaceae), Nat. Hist. Bull. Siam Soc. 49 (2001) 77-80.

Rehse T. and Kress W. J. - Distichochlamys rubrostriata (Zingiberaceae), a new species from northern Vietnam, Brittonia 55 (2003) 205-208. https://doi.org/10.1663/0007-196X(2003)055[0205:DRZANS]2.0.CO;2.

Quoc Binh Nguyen and Jana Leong-Škorničková - Distichochlamys benenica (Zingiberaceae), a new species from Vietnam, Gardens’ Bull Singapore 64 (2012) 195-200.

Pham T. V., Hoang H. N. T., Nguyen H. T., Nguyen H. M., Huynh C. T., Vu T. Y., Do A. T., Nguyen N. H., Do B. H. - Anti-inflammatory and antimicrobial activities of compounds isolated from Distichochlamys benenica, BioMed. Res. Int. (2021) 1-10. 10.1155/2021/6624347.

Pham V. T., Truong V., Dang T. T. N., Vo Q. H., and Ho V. D. - Chemical composition of the essential oils of Distichochlamys citrea leaves collected from Central Viet Nam, Vietnam J. Chem. 55 (2017) 358-362.

Tran V. C, Cao L. T. T, Hieu T.-T., Nguyen T. A. N, Nguyen T. T. - Phytochemical constituents isolated from Distichochlamys citrea M.F. Newman rhizomes and their antioxidant and α-glucosidase inhibitory activities, Nat. Prod. Commun. 18 (5) (2023) 1-7. https://doi.org/10.1177/1934578X231177878.

Nguyen V. H., To D. C, Phan T. Q., Thanh Q. B, Nguyen T. T. H., Nguyen T. T., Doan D. T., Nguyen T. T. N., Nguyen M. T., Tran V. C., et al. - Antimicrobial properties of Distichochlamys citrea M.F. Newman rhizome n-hexane extract against Streptococcus pyogenes: Experimental evidences and computational screening, Chemistry Select 7 (2022) e202200680. https://doi.org/10.1002/slct.202200680.

Thinh B. B., Hanh D. H., Hung N., and Thin D. B. - Comparison of yield, chemical composition and antimicrobial activity of Distichochlamys citrea rhizome essential oils obtained by different extraction methods, Moscow Univ. Chem. Bull. 77 (2022) 300-305. https://doi.org/10.3103/S0027131422050108.

Van Chen T., Cuong T. D., Quy P. T., Bui T. Q., Van Tuan L., Van Hue N., Thanh Triet N., Viet Ho D., Chi Bao N., Ai Nhung N. T. - Antioxidant activity and α-glucosidase inhibitability of Distichochlamys citrea M.F. Newman rhizome fractionated extracts: in vitro and in silico screenings, Chem. Pap. 76 (2022) 5655-5675. https://doi.org/10.1007/ s11696-022-02273-2.

Le T. H., Dao T. M. C., Nguyen V. H., Do N. D., and Isiaka A. O. - Volatile constituents of Distichochlamys citrea M. F. Newman and Distichochlamys orlowii K. Larsen M. F. Newman (Zingiberaceae) from Viet Nam, J. Med. Plants Res. 11 (2017) 188-193. https://doi.org/10.5897/JMPR2016.6337.

Chen W., Weng S. Q., Lv M. G., Chen W. Q., Bi Z. F., Chen H. Y., Luo T., Hu H., and Liao W. B. - β-Elemene inhibits human sperm function by affecting sperm vitality and intracellular calcium, Cell Physiol. Biochem. 51 (2018) 2019-2029. 10.1159/000495821.

Qi F., Yan Q., Zheng Z., Liu J., Chen Y., Zhang G. - Geraniol and geranyl acetate induce potent anticancer effects in colon cancer Colo-205 cells by inducing apoptosis, DNA damage and cell cycle arrest, J. BUON 23 (2018) 346-352.

Scandiffio R., Geddo F., Cottone E., Querio G., Antoniotti S., Gallo M. P., Maffei M. E., Bovolin P. - Protective effects of (E)-β-caryophyllene (BCP) in chronic inflammation, Nutrients 12 (2020) 3273. 10.3390/nu12113273.

Qui B., Wei F., Su J., Hao W., Zhou J., Zhao J., Wang Y., Qu Z. - The effects of β-Pinene, a pine needle oil monoterpene, on adenovirus type 3, Bull. Exp. Biol. Med. 172 (3) (2022) 345-351. 10.1007/s10517-022-05390-w.

Luyen L., Thom V., Huong L. T., Huong D. L., and Anh N. V. - Inhibitory effect on human platelet aggregation, antioxidant activity, and phytochemicals of Canna warszewiczii (A. Dietr) Nb. Tanaka, Phcog. Res. 12 (2020) 47.

Le H. L., Nguyen T. M. H., Vu T. T., Nguyen T. T. O., Ly H. D. T., Le N. T., Nguyen V. H., Nguyen T. V. A. - Potent antiplatelet aggregation, anticoagulant and antioxidant activity of aerial Canna x generalis L.H Bailey & E.Z Bailey and its phytoconstituents, South Afr. J. Botany. 147 (2022) 882-893. https://doi.org/10.1016/j.sajb.2022.03.035.

Ahmad A., Rehman M. U., Wali A. F., El-Serehy H. A., Al-Misned F. A., Maodaa S. N., Aljawdah H. M., Mir T. M., Ahmad P. - Box–Behnken response surface design of polysaccharide extraction from Rhododendron arboreum and the evaluation of its antioxidant potential, Molecules 25 (2020) 1-12. 10.3390/molecules25173835.

Kang M. J., Shin S., Park J. N., and Lee S. S. - The effects of polyunsaturated: saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in rats, British J. Nutr. 94 (2005) 526-532. 10.1079/bjn20051523.

Gayathiri S. N., Sivamurugan E., Manivasagaperumal M., Jayanthi R., Prakash P., Selvam K. - Molecular docking based screening dynamics for plant based identified potential compounds of PDE12 inhibitors, Curr. Res. Green Sustain. Chem. 4 (2021) 100122. https://doi.org/10.1016/j.crgsc.2021.100122.

Yu, L. - Free radical scavenging properties of conjugated linoleic acids, J. Agric. Food Chem. 49 (2001) 3452-3456. https://doi.org/10.1021/jf010172v.

Lv H., Ren D., Yan W., Wang Y., Liu, H., Shen M. - Linoleic acid inhibits Lactobacillus activity by destroying cell membrane and affecting normal metabolism, J. Sci. Food Agric. 100 (2020) 2057-2064. 10.1002/jsfa.10228.

Muhamad I. I., Hassan N. D., Mamat S. N. H., Nawi N. M., Rashid W. A., Tan N. A. - Extraction technologies and solvents of phytocompounds from plant materials : Physicochemical characterization and identification of ingredients and bioactive compounds from plant extract using various instrumentations, Ingredients Extraction by Physicochemical Methods in Food (2017) 523-560. https://doi.org/10.1016/B978-0-12-811521-3.00014-4.

Oforma C., Udourioh G. & Ojinnaka C. - Characterization of essential oils and fatty acids composition of stored ginger, J. Appl. Sci. Environ. Manag. 23 (2019) 2231-2238. 10.4314/jasem.v23i12.22.

Swanson. D., Block R., and Mousa S. A. - Omega-3 fatty acids EPA and DHA: Health benefits throughout life, Am. Soc. Nutr. 3 (2012) 1-7 (2012). 10.3945/an.111.000893.

Preston Mason R. - New insights into mechanisms of action for Omega-3 fatty acids in atherothrombotic cardiovascular disease, Curr. Atheroscler. Rep. 21 (2019) 2. https://doi.org/10.1007/s11883-019-0762-1.

Calder P. C. - Omega-3 fatty acids and inflammatory processes, Nutrients 2 (2010) 355-374. 10.3390/nu2030355.

Chen J., Garssen J., and Redegeld F. - The efficacy of bortezomib in human multiple myeloma cells is enhanced by combination with omega-3 fatty acids DHA and EPA: Timing is essential, Clin. Nutr. 40 (2021) 1942-1953. 10.1016/j.clnu.2020.09.009.

Liu Y., Ragone D., and Murch S. J. - Breadfruit (Artocarpus altilis): a source of high-quality protein for food security and novel food products, Amino Acids 47 (2015) 847-856. 10.1007/s00726-015-1914-4.

Chandana T. and P. Venkatesh Y. - Occurrence, functions and biological significance of arginine-rich proteins, Curr. Protein Peptide Sci. 17 (2016) 507-516. 10.2174/ 1389203717666151201192348.

Ghasemzadeh A., Jaafar H. Z. E., and Rahmat A. - Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe), Molecules 15 (2010) 4324-4333. 10.3390/molecules15064324.

Ali A. M. A., El-Nour M. E. M., and Yagi S. M. - Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors, J Genet Eng Biotechnol. 16 (2018) 677-682. 10.1016/j.jgeb.2018.03.003.

Yeh H., Chuang C., Chen H., Wan C., Chen T., Lin L. - Bioactive components analysis of two various gingers (Zingiber officinale Roscoe) and antioxidant effect of ginger extracts, LWT - Food Sci Technol. 55 (2014) 329-334. https://doi.org/10.1016/j.lwt.2013.08.003.