Isolation, characterization, and nitric oxide production inhibitory activities of the metabolites from the Psidium guava L. in Viet Nam

Ngo Van Hieu; Pham Van Cong; Ngo Viet  Duc; Do Thi Thu Hien; Duong Thanh Nam; Le Van Nhan; Hoang Minh Tao; Vu Thi Trang; Nguyen Quang Trung; Nguyen Tien Dat; Pham Thi Mai; Hoang Le Tuan Anh

doi:10.15625/2525-2518/18298

Author affiliations

Authors

Ngo Van Hieu Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0003-2463-2224
Pham Van Cong Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0002-4098-0301
Ngo Viet Duc Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Do Thi Thu Hien Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Duong Thanh Nam Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Le Van Nhan Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Hoang Minh Tao Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Vu Thi Trang Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Quang Trung Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Tien Dat Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Pham Thi Mai Nha Trang University (NTU), 02 Nguyen Dinh Chieu, Nha Trang, Khanh Hoa, Viet Nam
Hoang Le Tuan Anh Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0002-1274-8759

DOI:

https://doi.org/10.15625/2525-2518/18298

Keywords:

Psidium guajava L, NO production inhibitory activities, avicularin, guaijaverin, casuarinin, 4-methoxy-3,5-dihyroxybenzoic acid

Abstract

Thirteen compounds, including catechin (1), gallocatechin (2), quercetin (3), myricetin (4), kaempferol 3-O-α-ʟ-arabinofuranoside (5), avicularin (6), guaijaverin (7), quercetin-3-O-β-ᴅ-galactopyranoside (8), quercetin-3-O-β-ᴅ-glucopyranoside (9), quercetin-3-O-β-ᴅ-glucuronide (10), casuarinin (11), ursolic acid (12), and 4-methoxy-3,5-dihydroxybenzoic acid (13) were isolated from the methanolic extract of the leaves Psidium guajava L. by using various chromatographic techniques. Moreover, the structures of these compounds were continuously elucidated by modern spectroscopic methods (MS, ¹H/¹³C-NMR) and compared with those of the reported data. In addition, all the isolated compounds were futher evaluated for their inhibitory effects on nitric oxide (NO) production in LPS-induced RAW cells with N^G-Methyl-L-arginine acetate (L-NMMA) as the positive control. Among of them, compounds 3, 8, 10, and 11 showed inhibitory effects on NO production within IC₅₀ values of 19.30 – 42.86 µM, compared to positive control: IC₅₀ 7.90 ± 0.63 µM. Interestingly, compound 13 was first isolated from Psidium guajava L.

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