Extracellular L-asparaginase productive potential of the Priestia megaterium strain GB911 from Khanh Hoa sea of Viet Nam

Do Thi Phuong; Nguyen Mai Anh; Nguyen Thi Nga; Vu Thi Thu Huyen; Nguyen Thi Cuc; Trieu Ha Phuong; Le Thi Hong Minh; Do Thi Thao

doi:10.15625/2525-2518/17906

Author affiliations

Authors

Do Thi Phuong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Mai Anh Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Thi Nga Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Vu Thi Thu Huyen Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Thi Cuc Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Trieu Ha Phuong Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Le Thi Hong Minh Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Do Thi Thao Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0002-8760-264X

DOI:

https://doi.org/10.15625/2525-2518/17906

Keywords:

Priestia megaterium, L-asparaginase, cytotoxicity, extracellular enzyme, marine bacteria

Abstract

Currently, L-asparaginase isolated from terrestrial microorganisms has been employed for anticancer industrial production. This enzyme has the function of inhibiting the growth of acute lymphoblastic leukemia, Hodgkin's lymphomas and other cancers. In this study, for the first time, L-asparaginase (ASNase) productive capacity of forty marine bacteria originating from Khanh Hoa sea of Viet Nam was screened and reported. Among those, twenty eight isolates were clarified for their extracellular ASNase activity based on their ability to form a pink zone around colonies on asparagine agar plates. As a result, four strains GB910, GB911, GB976 and GB982 showed high secreted extracellular ASNase activity, ranging from 8.64 to 13.22 IU/mg. Among these four most potential strains, GB911 exhibited better enzyme production capacity and was subjected to taxonomy identification. According to the 16S rRNA gene sequence on the GenBank database, strain GB911 was identified as Priestia megaterium and registered in the GenBank database with accession number MW407014. The purified enzyme obtained from strain GB911 exhibited strong cytotoxic activity against five monolayer and suspension cancerous cell lines with IC₅₀ values ranging from 39 mU/mL to 98 mU/mL. The strain could serve as a promising source for industrial production of ASNase for clinical chemotherapeutic treatment.

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