Preparation and characterization of materials based on fish scale collagen and polyphenols extracted from Camellia chrysantha

Nguyen Thuy Chinh; Mai Duc Huynh; Ly Thi Ngoc Lien; Tran Thi Mai; Thai Hoang

doi:10.15625/2525-2518/16530

Author affiliations

Authors

Nguyen Thuy Chinh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam https://orcid.org/0000-0001-8016-3835
Mai Duc Huynh Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Ly Thi Ngoc Lien Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Tran Thi Mai Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Thai Hoang Institute for Tropical Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16530

Keywords:

Tea polyphenols, Camellia chrysantha, fish scale collagen

Abstract

This paper presents the preparation and some characteristics of collagen/polyphenols materials containing different polyphenols contents. Collagen was extracted from fresh-water fish scales and polyphenols were extracted from Camellia chrysantha (Tam Dao, Vinh Phuc, Viet Nam). A comparison between these polyphenols and green tea polyphenols (commercial product) was carried out in this study. Functional groups and morphology of collagen/polyphenols materials were determined by Infrared spectroscopy (IR) and scanning electron microscope (SEM). Ultraviolet-visible spectroscopy (UV-Vis) was used to evaluate the release of polyphenols from collagen/polyphenols materials in simulated body fluids. In addition, the anti-oxidation ability of collagen/polyphenols materials was also investigated by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging method. The obtained results showed that polyphenols with the size of ca. 100 nm were dispersed and adhered well to the collagen matrix. The release of polyphenols from the materials follows two stages: fast release during the first 2 hours of testing and controlled release in the following hours. The content of polyphenols released from the collagen/polyphenols materials in pH 2.0 solution was higher than that in pH 7.4 solution. After 8 hours of testing, the content of polyphenols released from the collagen/polyphenols materials reached 15-65 % depending on the polyphenols content, type of polyphenols and pH of solution. The anti-oxidation ability of polyphenols extracted from Camellia chrysantha is better than that of green tea polyphenols and collagen/polyphenols materials.

Downloads

Download data is not yet available.

References

Chung S. Y., Joshua D. L., Shengmin S. - Antioxidative and anti-carcinogenic activities of tea polyphenols, Arch. Toxicol. 83 (1) (2009) 11. 10.1007/s00204-008-0372-0. DOI: https://doi.org/10.1007/s00204-008-0372-0

Shengmin S., Ill Y., Brian B., Chi-Tang H., Chung S. Y. - Autoxidative quinone formation in vitro and metabolite formation in vivo from tea polyphenol (-)-epigallocatechin-3-gallate: studied by real-time mass spectrometry combined with tandem mass ion mapping, Free Radic. Biol. Med. 43 (2007) 362–371. 10.1016/j.freeradbiomed.2007.04.008. DOI: https://doi.org/10.1016/j.freeradbiomed.2007.04.008

Kazunari K., Masaaki K., Naoki M., Takashi S., Masatake T. - Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action, Free Radic. Biol. Med. 27 (1999) 855–863. 10.1016/s0891-5849(99)00133-1. DOI: https://doi.org/10.1016/S0891-5849(99)00133-1

Yan Z., Zhong Y., Duan Y., Chen Q., Li F. - Antioxidant mechanism of tea polyphenols and its impact on health benefits, Anim. Nutr. 6 (2) (2020) 115-123. https://doi.org/10.1016/j.aninu.2020.01.001. DOI: https://doi.org/10.1016/j.aninu.2020.01.001 https://doi.org/10.1016/j.aninu.2020.01.001.">

Barhé T. A., Feuya Tchouya G. R. - Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals, Arab. J. Chem. 9 (1) (2016) 1-8. https://doi.org/10.1016/j.arabjc.2014.11.048. DOI: https://doi.org/10.1016/j.arabjc.2014.11.048 https://doi.org/10.1016/j.arabjc.2014.11.048.">

Song L., Wang X., Zheng X., Huang D. - Polyphenolic antioxidant profiles of yellow camellia, Food Chem. 129 (2011) 351–357. https://doi.org/10.1016/j.foodchem.2011.04.083. DOI: https://doi.org/10.1016/j.foodchem.2011.04.083 https://doi.org/10.1016/j.foodchem.2011.04.083.">

Verena S., Henryk D., Karl S., Mario L. - Molecular targets of tea polyphenols in the cardiovascular system, Cardiovasc. Res. 73 (2007) 348–358. DOI: https://doi.org/10.1016/j.cardiores.2006.08.022

Lin J. N., Lin H. Y., Yang N. S. - Chemical constituents and anticancer activity of yellow camellias against MDA-MB-231 human breast cancer cells, Food Chem. 61 (2013) 9638 − 9644. https://doi.org/10.1021/jf4029877. DOI: https://doi.org/10.1021/jf4029877 https://doi.org/10.1021/jf4029877.">

Yokozawa T., Nakagawa T., Kitani K. - Antioxidative activity of green tea polyphenol in cholesterol-fed rats, J. Agric. Food Chem. 50 (12) (2002) 3549-3552. 10.1021/jf020029h. DOI: https://doi.org/10.1021/jf020029h

Ngo T. T. - Study on characteristics and determination of polyphenol, EGCG content and assessment of some in vitro biological effects of golden flower tea collected in Ba Che, Quang Ninh, Master's thesis in Pharmacology, Hanoi University of Pharmacy, 2016, (in Vietnamese).

Nguyen T. H. L. - Study on botanical characteristics, chemical composition and biological effects of golden flower tea in Thai Nguyen, Pharmacist Graduation Course, Hanoi University of Pharmacy, 2016, (in Vietnamese).

Nguyen T. C., Nguyen T. H. L, Nguyen T. T. T, Tran T. M, Thach T. L., Le D. G., Nguyen Quang Tung, Thai H. - Characteristic and properties of chitosan/alginate polymer blend carrying lovastatin drug, Vietnam J. Sci. Technol. 54 (2B) (2016) 118.

Tran D. L, Tran V. H., Mai T. T, Ha P. T., Nguyen H. B., Thai H., Vu D. H., Pham G. D., Nguyen X. P., Park J. K. - Biomedical and environmental applications of chitosan-based nanomaterials, J. Chitin Chitosan 1 (2011) 7-14.

Yang C., Wu H., Wang J. - Formulation and evaluation of controlled-release of steroidal saponins-loaded collagen microspheres, Mater. Technol. 34 (9) (2019) 534-539. https://doi.org/10.1080/10667857.2019.1591727. DOI: https://doi.org/10.1080/10667857.2019.1591727 https://doi.org/10.1080/10667857.2019.1591727.">

Zhang L., Wang Y., Xia T., Yu Q., Zhang Q., Yang Y., He Q. - Suppression for lung metastasis by depletion of collagen I and lysyl oxidase via losartan assisted with paclitaxel-loaded pH-sensitive liposomes in breast cancer, Drug Deliv. 23 (8) (2016) 2970–2979. 10.3109/10717544.2015.113279. DOI: https://doi.org/10.3109/10717544.2015.1132798

Ai A., Behforouz A., Ehterami A., Sadeghvaziri N., Jalali S., Farzamfar S., Yousefbeigi A., Goodarzi A., Salehi M., Ai J. - Sciatic nerve regeneration with collagen type I hydrogel containing chitosan nanoparticle loaded by insulin, Int. J. Polym. Mater. Polym. Biomater. 68 (18) (2019) 1133-1141. https://doi.org/10.1080/00914037.2018.1534114. DOI: https://doi.org/10.1080/00914037.2018.1534114 https://doi.org/10.1080/00914037.2018.1534114.">

Gholam R. M., Amirabbas M., Moslem S., Mohammad S. - Magnetic- and pH-responsive -carrageenan/chitosan complexes for controlled release of methotrexate anticancer drug, Int. J. Biol. Macromol. 97 (2017) 209–217. 10.1016/j.ijbiomac.2017.01.012. DOI: https://doi.org/10.1016/j.ijbiomac.2017.01.012

Debnath K., Shekhar S., Kumar V., Jana N. R., Jana N. R. - Efficient inhibition of protein aggregation, disintegration of aggregates, and lowering of cytotoxicity by green tea polyphenol-based self-assembled polymer nanoparticles, ACS Appl. Mater. Interfaces 8 (31) (2016) 20309-18. 10.1021/acsami.6b06853. DOI: https://doi.org/10.1021/acsami.6b06853

Shirode A. B., Bharali D. J., Nallanthighal S. - Nanoencapsulation of pomegranate bioactive compounds for breast cancer chemoprevention, Int. J. Nanomed. 10 (2015) 475–484. 10.2147/IJN.S65145. DOI: https://doi.org/10.2147/IJN.S65145

Kim Y. J., Park M. R., Kim M. S., Kwon O. H. - Polyphenol-loaded polycaprolactone nanofibers for effective growth inhibition of human cancer cells, Mater. Chem. Phys. 133 (2012) (2-3), 674–680. 10.1016/j.matchemphys.2012.01.050. DOI: https://doi.org/10.1016/j.matchemphys.2012.01.050

Cao P., Vadhanam M. V., Spencer W. A., Cai J., Gupta R. C. - Sustained systemic delivery of green tea polyphenols by polymeric implants significantly diminishes benzo[α]pyrene-induced DNA adducts, Chem. Res. Toxicol. 24 (6) (2011) 877-886. 10.1021/tx2000625. DOI: https://doi.org/10.1021/tx2000625

Jin W., Xu W., Ge H., Li Y., Li B. - Polyphenol–gelatin nanoparticles as reductant and stabilizer for one-step synthesis of gold nanoparticles and their interfacial behavior, RSC Adv. 5 (34) (2015) 26496–26503. 10.1039/C5RA02566D. DOI: https://doi.org/10.1039/C5RA02566D

Nguyen T. C., Vu Q. M., Vu Q. T., Tran D. L., Mai D. H., Nguyen Q. T., Nguyen D. T., Thai H. - Characterization of collagen derived from tropical freshwater carp fish scale wastes and its amino acid sequence, Nat. Prod. Commun. 14 (7) (2019) 1–12. https://doi.org/10.1177/1934578X19866288. DOI: https://doi.org/10.15625/2525-2518/57/3B/14044 https://doi.org/10.1177/1934578X19866288.">

Sijin C., Hui C., Quanning X., Bihong H., Junde C., Fang H., Kaikai B., Jianlin H., Ruizao Y., Hao W. - Rapid isolation of high purity pepsin-soluble type I collagen from scales of red drum fish, Food Hydrocoll. 52 (2016) 468–477. https://doi.org/10.1016/j.foodhyd.2015.07.027. DOI: https://doi.org/10.1016/j.foodhyd.2015.07.027 https://doi.org/10.1016/j.foodhyd.2015.07.027.">

Kai M., Klaus H. V., Sebastian L., Ralf H., Andreas R. and Ulf-Peter H. - Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements, Sensors 7 (2007) 2080-2095. 10.3390/s7102080. DOI: https://doi.org/10.3390/s7102080