Response surface methodology optimization of polyhydroxyalkanoate by recombinant bacillus megaterium PPSPHAR1/1 strain using fish processing waste production

Pham Thanh Huyen; Bach Thi Mai Hoa; Nguyen Trong Linh; La Thi Huyen; Nguyen Thi Da

doi:10.15625/2525-2518/16270

Author affiliations

Authors

Pham Thanh Huyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Bach Thi Mai Hoa Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Trong Linh Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
La Thi Huyen Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam
Nguyen Thi Da Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/16270

Keywords:

Bacillus megaterium, Polyhydroxybutyrate, PHB, submerged fermentation, fish processing waste, oil fish

Abstract

Polyhydroxyalkanoates (PHAs) are biomaterials that are accumulated intracellularly by bacterial cells in response to nutrient imbalances under environmental stress. Polyhydroxybutyrate (PHB) is a bioplastic that is of interest to research to find an alternative to fossil-derived plastics. The optimal physical and nutritional conditions for PHB production were investigated by varying one variable at a time. To achieve maximum PHA production, the culture conditions for B. megaterium pPSPHAR1/1 were optimized through response surface methodology (RSM). The final optimum fermentation conditions included: 13.34 (g/L) glucose; 7.28 (g/L) Na₂HPO₄_;4.45 (g/L) K₂HPO₄; MgSO₄0.2; 2 (g/L) (NH₄)₂SO₄; NH₄Fe(III) citrate 0.005 %; acid citric 0.1 %; 2 Ml of trace minerals, 3 (%w/v) fish oil; 1.3 (%v/v) fish extract; inoculum size, 10 % (v/v)and temperature of 37 ^oC for 72 h. Using the optimal medium, the PHB production of this recombinant strain accumulated a PHB content of about 76.2 % per cell dry weight in a 5 L stirred bioreactor.

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