RESPONSE SURFACE OPTIMIZED EXTRACTION OF TRITERPENOIDS FROM RED VIETNAMESE GANODERMA LUCIDUM AND ANTICANCER EVALUATION OF THE EXTRACT

Authors

  • Tran Do Dat Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Nguyen Tan Tai Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Nguyen Thi Linh Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Phan Le Thao My Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Ngo Thi Thuy Linh Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Nguyen Thi Kim Ngan Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Vuong Hoai Thanh Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Nguyen Duc Viet Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Hoang Minh Nam Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Mai Thanh Phong Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM
  • Nguyen Huu Hieu Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM

DOI:

https://doi.org/10.15625/2525-2518/59/2/15485

Keywords:

Ganoderma lucidum, ultrasound – assisted extraction, response surface methodology, triterpenoids

Abstract

Triterpenoids are considered to be the major pharmaceutical active compounds found in the macrofungus Ganoderma lucidum (G. lucidum). Extraction conditions of triterpenoids from the Vietnamese red G. lucidum were optimized by response surface methodology using ultrasound –assisted extraction (UAE) method. A Box – Behnken experimental design was used to determine the simultaneous influences of three independent variables, namely solvent-to-material ratio, extraction time, and ultrasonic power on the yield of triterpenoids. The highest yield of triterpenoids obtained under optimum conditions including extraction time of 55 min, ultrasonic power of 480 W, solvent-to-material ratio of 27:1 mL/g was 9.5768 ± 0.228 mg/g according to ultraviolet-visible spectroscopy (UV-Vis) analysis. Besides, the hot water extraction method was carried out in order to compare with UAE in terms of the yield of triterpenoids. The results of anticancer evaluation of the extract obtained indicated that with the half-maximal inhibitory concentration value of 67.25 ± 0.82 µg/mL on the human Hep-G2 liver cancer cell lines, triterpenoids extracted from G. lucidum could be regarded as a potential agent for medicinal treatment.

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Published

2021-03-16

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Section

Natural Products