High performance liquid chromatography assay for determination of moxifloxacin in human plasma

Luong Thi My Hanh; Nguyen Thi Minh Diep; Pham Thi Ngoc Mai; Nguyen Xuan Truong

doi:10.15625/2525-2518/57/3/13362

Author affiliations

Authors

Luong Thi My Hanh Faculty of Chemistry, Hanoi University of Science, VNU Ha Noi, 19 Le Thanh Tong street, Hoan Kiem, Ha Noi, Viet Nam
Nguyen Thi Minh Diep Department of Drug Quality Control, Phu Tho Medical and Pharmacy College, No. 2201, Hung Vuong street, Viet Tri, Phu Tho, Viet Nam
Pham Thi Ngoc Mai Faculty of Chemistry, Hanoi University of Science, VNU Ha Noi, 19 Le Thanh Tong street, Hoan Kiem, Ha Noi, Viet Nam
Nguyen Xuan Truong School of Chemical Engineering, Hanoi University of Science and Technology, No. 1, Dai Co Viet, Hai Ba Trung, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/2525-2518/57/3/13362

Keywords:

Moxifloxacin, protein precipitation, tricloroacetic acid, HPLC method, human plasma

Abstract

A simple reversed phase HPLC method with UV detection has been successfully developed and validated for determination of moxifloxacin in human plasma. The sample pretreatment involves only single-step protein precipitation with tricloroacetic acid. Moxifloxacin was measured in plasma using a validated HPLC method with UV detector at 295 nm, C18 column (25cm×4.5mm, 5µm), a mixture of phosphate buffer pH 4.0 and acetonitrile (70:30, v/v) as mobile phase at a flow rate of 0.8 ml/min. Retention time of moxifloxacin was found to be 7.4 min. The mean recovery for the drug was obtained 97.30%. The calibration curve was linear over the concentration range of 0.3 to 25.0 µg/mL with coefficient correlation of 0.9991. This method was successfully applied for therapeutic drug monitoring.

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