CONSTITUENTS AND INHIBITORY EFFECT ON HUMAN PATHOGENIC BACTERIA OF THE ROOTS OF SCUTELLARIA BAICALENSIS

Authors

  • Duc Long Le R&D Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry,No. 2 Pham Ngu Lao, Hoan Kiem, Hanoi, Viet Nam
  • Huu Tung Nguyen VNU School of Medicine and Pharmacy, Vietnam National University, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam
  • Thi Thom Nguyen Department of Pharmaceutical Chemistry and Pesticides Technology, School of Chemical Engineering, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hai Ba Trung, Hanoi, Viet Nam
  • Gyung Ja Choi Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, 141 Gajeong-Ro, Yuseong-Gu, Daejeon, 305-600, Republic of Korea
  • Dinh Hoang Vu Department of Pharmaceutical Chemistry and Pesticides Technology, School of Chemical Engineering, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hai Ba Trung, Hanoi, Viet Nam
  • Quang Le Dang R&D Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry,No. 2 Pham Ngu Lao, Hoan Kiem, Hanoi, Viet Nam https://orcid.org/0000-0001-7203-8081

DOI:

https://doi.org/10.15625/2525-2518/57/1/12886

Keywords:

activity, flavonoid, isolation, Scutellaria baicalensis

Abstract

Abstract-HCTN_16

Methanol extract of the roots of Scutellaria baicalensis effectively inhibited the bacterial growth of human pathogenic bacteria Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 21768 and Bacillus subtilis ATCC 6633 at MICs of 2,000 µg/mL. n-Hexane, ethyl acetate and aqueous residues were prepared by successively partitioning the methanol extract with n-hexane and ethyl acetate. Among them, only ethyl acetate layer showed antibiotic effect; whereas n-hexane and aqueous layers were inactive against tested bacteria. The ethyl acetate residue was fractionated by silica gel column chromatography to afford three flavonoids and an oligosaccharide. Their chemical structures were elucidated as wogonin (SB1), baicalein (SB4), baicalin (SB5) and tetrasaccharide (SB10) on the basis of the analysis of NMR and MS spectroscopic data. The isolates were evaluated for in vitro inhibitory effect against human pathogenic bacteria using micro dilution bioassay method. Baicalein (SB4) showed a broad-spectrum inhibition against various human pathogenic bacteria. In particular, it was found to potently inhibit S. aureus ATCC 6538 and B. cereus ATCC 21768 with MICs of 9.5 and 38 µg/mL, respectively. The study results demonstrated antibiotic effect of the extracts from the roots of S. baicalensis and characterization of compounds isolated from the plant materials.

Downloads

Download data is not yet available.

Author Biography

Quang Le Dang, R&D Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry,No. 2 Pham Ngu Lao, Hoan Kiem, Hanoi, Viet Nam

Director/Senior Researcher, R&D Center of Bioactive Compounds

References

Konoshima T., Kokumai M., Kozuka M., Iinuma M., Mizuno M., Tanaka T., Tokuda H., Nishino H., Iwashima A.- Studies on inhibitors of skin tumour promotion. XI. Inhibitory effects of flavonoids from Scutellaria baicalensis on epstein-barr virus activation and their anti-tumour-promoting activities, Chem. Pharm. Bull, 40(2) (1992) 531–533.

Guo Q., Zhao L., You Q., Yang Y., Gu H., Song G., Lu N., Xin J.- Anti-hepatitis B virus activity of wogonin in vitro and in vivo, Antiviral Res., 74 (2016) 16–24.

Chen X., Zhang C.- Inhibitory role of baicalin on human herpes virus type 6 in vitro, Rocedia Engineering, 37 (2012) 75–78.

Vu T.T., Kim H., Vu T.K., Le Dang Q., Nguyen H.T., Kim H., In K. S., Choi J. G., Kim J-.C.- In vitro antibacterial activity of selected medicinal plants traditionally used in Vietnam against human pathogenic bacteria, BMC Complement Altern. Med.,16 (32) (2016).

Delange D. M., Rico C. L. M., Canavaciolo V. G., Cuellar A. C., Oliver E. S.- Selective and high yield isolation of pure wogonin from aerial part of Scutellaria havanensis Jacq., Int. J. Pharm. Sci. Rev. Res., 30 (2015) 104–108.

Pegg R. B., Amarowicz R., Oszmianski J. - Confirming the chemical structure of antioxidativetri hydroxyflavones from Scutellaria baicalensis using modern spectroscopic methods, Pol. J. Food Nutr. Sci., 14 (2005) 43–50.

Zhou Y., Hirotani M., Yoshikawa T., Furuya T.- Flavonoids and phenylethanoids from hairy root cultures of Scutellaria baicalensis, Phytochem., 44 (1997), 83–87.

Ehrhardt S, Rittig F, Vogel M, Wray V, Skeries B.- Sucrosyl‐(1 → 2)‐β‐isomaltulose: enzymatic synthesis and structure determination. J. Carbohyd. Chem., 23 (2–3) (2004) 163–168.

Chan B. C., Ip M., Lau C. B., Lui S. L., Jolivalt C., Ganem-Elbaz C., Litaudon M., Reiner N. E., Gong H., See R. H., Fung K. P., Leung P. C.- Synergistic effects of baicalein with ciprofloxacin against Nora over-expressed Methicillin-Resistant Staphylococcus aureus (MRSA) and inhibition of MRSA pyruvate kinase, J.Ethnopharmacol.,137 (2011) 767–773.

Cai W., Fu Y., Zhang W., Chen X., Zhao J., Song W., Li Y., Huang Y., Wu Z., Sun R., Dong C., Zhang F.- Synergistic effects of baicalein with cefotaxime against Klebsiella pneumoniae through inhibiting Ctx-M-1 gene expression, BMC Microbiol.,16 (2016), 181.

Downloads

Published

2019-02-18

Issue

Section

Natural Products