ISOLATION AND SELECTION OF BACILLUSSTRAINS FOR THE PRODUCTION OF BIO-PRODUCTS USING IN AQUACULTURE

Abstract

SUMMARY

95 strains of bacilli  were isolated from the samples of shrimp ponds, theworm’s muck for shrimp’s food, and the digestive tract of shrimp. As a result, six strains of Bacilluswere selected. All the selected strains contain the active enzymes(protease, cellulase and amylase), which can strongly disintegrate the organic substances with (D-d ~ 16,5 – 24,5 mm). The strains BaD, BaPG, BaRT 9 and BaRT 6 were chosen for the production of bio-products in aquaculture. These  Bacillus  strains showed strong antagonistic properties against pathogenic and harmful bacteria, and high enzyme activities as well. The classification of these strains based on their  physiological characteristics and 16S rRNA sequence indicated that strain BaD which has the similarity rate of 100% to  Bacillus subtilis  EBS05 (FJ876834), strain BaPG with the similarity rate of 99% to the  Bacillus subtilis BFE 5314 (GU250453.1), strain BaPG 3 with the similarity rate of 99% to the  Bacillus subtilis GB16 16S AY11607.1), strain BaRT 6 with the similarity rate of 99% to the  Bacillus licheniformisSL2 (GQ184581.1); The strain BaRT 4 with the similarity rate of 89% to the Bacillus sp.B26(2008) (EU362172.1).