Impedimetric immunosensor for atrazine detection based on polypyrrole nanowires

Nguyen Van Anh, Nguyen Van Quan, Nguyen Hai Binh, Nguyen Le Huy, Le Trong Huyen, Tran Dai Lam
Author affiliations

Authors

  • Nguyen Van Anh School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
  • Nguyen Van Quan School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
  • Nguyen Hai Binh Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road., Cau Giay, Ha Noi, Viet Nam
  • Nguyen Le Huy School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
  • Le Trong Huyen School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
  • Tran Dai Lam Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road., Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/0866-708X/52/6/3595

Abstract

Animpedimetric immunosensor based on polypyrrole (PPy) nanowires was developedand applied for atrazine (ATZ) herbicide detection. In this work, the PPynanowires were synthesized by an electrochemical free-template method onto aninterdigitated platinum micro-electrode (IDµE). The immunosensors were preparedby immobilization of monoclonal anti-atrazine antibody (a-ATZ) onto a PPy surface using glutaraldehyde asa cross-linker. The electrodes were characterized using scanning electronmicroscopy (SEM), cyclic voltammetry (CV) and electrochemical impedancespectroscopy (EIS) techniques. The ATZ analysis is based on the immunoreactionof free ATZ and a-ATZ attached on top of the IDµE area (fingersand inter-digits space) by means of EIS. Thanks to immunoreagent specificity,these sensors exhibit high selectivity and sensitivity for ATZ detection with athreshold of 10 ng/mL

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Published

14-01-2015

How to Cite

[1]
N. V. Anh, N. V. Quan, N. H. Binh, N. L. Huy, L. T. Huyen, and T. D. Lam, “Impedimetric immunosensor for atrazine detection based on polypyrrole nanowires”, Vietnam J. Sci. Technol., vol. 52, no. 6, pp. 777–785, Jan. 2015.

Issue

Vol. 52 No. 6 (2014)

Section

Articles

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