Impedimetric immunosensor for atrazine detection based on polypyrrole nanowires

Nguyen Van Anh; Nguyen Van Quan; Nguyen Hai Binh; Nguyen Le Huy; Le Trong Huyen; Tran Dai Lam

doi:10.15625/0866-708X/52/6/3595

Impedimetric immunosensor for atrazine detection based on polypyrrole nanowires

Nguyen Van Anh, Nguyen Van Quan, Nguyen Hai Binh, Nguyen Le Huy, Le Trong Huyen, Tran Dai Lam

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Authors

Nguyen Van Anh School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Van Quan School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
Nguyen Hai Binh Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road., Cau Giay, Ha Noi, Viet Nam
Nguyen Le Huy School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
Le Trong Huyen School of Chemical Engineering, Hanoi University of Science and Technology, Số 1, Dai Co Viet Road, Hai Ba Trung, Ha Noi, Viet Nam
Tran Dai Lam Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road., Cau Giay, Ha Noi, Viet Nam

DOI:

https://doi.org/10.15625/0866-708X/52/6/3595

Abstract

Animpedimetric immunosensor based on polypyrrole (PPy) nanowires was developedand applied for atrazine (ATZ) herbicide detection. In this work, the PPynanowires were synthesized by an electrochemical free-template method onto aninterdigitated platinum micro-electrode (IDµE). The immunosensors were preparedby immobilization of monoclonal anti-atrazine antibody (a-ATZ) onto a PPy surface using glutaraldehyde asa cross-linker. The electrodes were characterized using scanning electronmicroscopy (SEM), cyclic voltammetry (CV) and electrochemical impedancespectroscopy (EIS) techniques. The ATZ analysis is based on the immunoreactionof free ATZ and a-ATZ attached on top of the IDµE area (fingersand inter-digits space) by means of EIS. Thanks to immunoreagent specificity,these sensors exhibit high selectivity and sensitivity for ATZ detection with athreshold of 10 ng/mL

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Published

14-01-2015

How to Cite

[1]

N. V. Anh, N. V. Quan, N. H. Binh, N. L. Huy, L. T. Huyen, and T. D. Lam, “Impedimetric immunosensor for atrazine detection based on polypyrrole nanowires”, Vietnam J. Sci. Technol., vol. 52, no. 6, pp. 777–785, Jan. 2015.

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Vol. 52 No. 6 (2014)

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