Antioxidant activity of extract from some squid species in Khanh Hoa

Nguyen Phuong Anh, Pham Xuan Ky, Dao Viet Ha, Le Ho Khanh Hy, Doan Thi Thiet, Phan Bao Vy
Author affiliations

Authors

  • Nguyen Phuong Anh Institute of Oceanography, VAST, Vietnam
  • Pham Xuan Ky Institute of Oceanography, VAST, Vietnam
  • Dao Viet Ha Institute of Oceanography, VAST, Vietnam
  • Le Ho Khanh Hy Institute of Oceanography, VAST, Vietnam
  • Doan Thi Thiet Institute of Oceanography, VAST, Vietnam
  • Phan Bao Vy Institute of Oceanography, VAST, Vietnam

Keywords:

Extract, squid, antioxidant, FTIR, SDS-PAGE.

Abstract

The antioxidant activities against DPPH free radical of crude extracts using ethyl acetate and methanol from ink sac, muscles, and bones of 5 squid species (Uroteuthis chinensis, Uroteuthis sibogae, Uroteuthis duvaucelii, Sepia esculenta, Sepioteuthis lessoniana) collected in Khanh Hoa waters were investigated. These activities ranged from 4.21% (ethyl acetate extract from ink of U. chinensis) to 54.51% (methanol extract from muscle of S. esculenta.). The functional group analysis by infrared adsorption spectrum (FTIR) in these crude extracts revealed the absorption peaks of melanin in ink, polysaccharide in bone and protein in muscle extracts. In addition, the SDS-PAGE result of some methanol extracts showed the presence of proteins with a molecular weight of 30-150 kDa.

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References

[1] Guyton, K. Z., Bhan, P., Kuppusamy, P., Zweier, J. L., Trush, M. A., and Kensler, T. W., 1991. Free radical-derived quinone methide mediates skin tumor promotion by butylated hydroxytoluene hydroperoxide: expanded role for electrophiles in multistage carcinogenesis. Proceedings of the National Academy of Sciences, 88(3), 946–950. https://doi.org/10.1073/pnas.88.3.946.

[2] Papas, A., 1999. Diet and antioxidant status. Food and Chemical Toxicology, 37(9–10), 999–1007.

[3] Roesijadi, G., Kielland, S., and Klerks, P., 1989. Purification and properties of novel molluscan metallothioneins. Archives of Biochemistry and Biophysics, 273(2), 403–413. https://doi.org/10.1016/0003-9861(89)90499-2.

[4] Madhu, V., Sivaperumal, P., Kamala, K., Ambekar, A. A., and Kulkarni, B. G., 2014. Antibacterial and antioxidant activities of the tissue extract of Perna viridis Linnaeus, 1758 (Mollusca: Bivalvia) from versova coast, Mumbai. Int J Pharmacy Pharmaceutical Sci., 6, 704–707.

[5] Hasan, T., Wahab, A. W., Djide, N., and Zakir, M., 2015. Antioxidant Activity of Bioactive Protein of Kerang Kepah (Atactodea striata) from South Sulawesi. American Journal of Biomedical and Life Sciences, 3(6), 111–114.

[6] Arumugasamy, K., and Cyril, R., 2017. Cytotoxicity, Antibacterial and Antioxidant Activities of the Tissue Extracts of Marine Gastropod Hemifusus Pugilinus (Born, 1778) J. Chem. Pharm. Res, 9(10), 267–274.

[7] Luo, P., and Liu, H., 2013. Antioxidant ability of squid ink polysaccharides as well as their protective effects on deoxyribonucleic acid DNA damage in vitro. African Journal of Pharmacy and Pharmacology, 7(21), 1382–1388.

[8] Subhapradha, N., Ramasamy, P., Seedevi, P., Shanmugam, V., Srinivasan, A., and Shanmugam, A. 2014. Extraction, characterization and its antioxidant efficacy of polysaccharides from Sepia aculeata (Orbigny, 1848) cuttlebone. African Journal of Biotechnology, 13(1), 138–144. https://doi.org/10.5897/AJB2013.12893.

[9] Ponnusamy, K., Kamala, K., Munilkumar, S., and Pal, A. K., 2016. Antioxidant Properties from Tissue Extract of Cephalopods around Madras Atomic Power Station, Kalpakkam Coast. Int. J. Pharm. Res. Health Sci, 4, 1086–1091.

[10] Liu, H., Luo, P., Chen, S., and Shang, J., 2011. Effects of squid ink on growth performance, antioxidant functions and immunity in growing broiler chickens. Asian-Australasian Journal of Animal Sciences, 24(12), 1752–1756.

[11] Fahmy, S. R., and Soliman, A. M., 2013. In vitro antioxidant, analgesic and cytotoxic activities of Sepia officinalis ink and Coelatura aegyptiaca extracts. African Journal of Pharmacy and Pharmacology, 7(22), 1512–1522. https://doi.org/10.5897/AJPP2013.3564.

[12] Vate, N. K., and Benjakul, S., 2013. Antioxidative activity of melanin-free ink from splendid squid (Loligo formosana). International Aquatic Research, 5(1), 9. https://doi.org/10.1186/2008-6970-5-9.

[13] Vate, N. K., Benjakul, S., and Agustini, T. W., 2015. Application of melanin‐free ink as a new antioxidative gel enhancer in sardine surimi gel. Journal of the Science of Food and Agriculture, 95(11), 2201–2207. https://doi.org/10.1002/jsfa.6934.

[14] Sudhakar, S., and Nazeer, R. A., 2015. Preparation of potent antioxidant peptide from edible part of shortclub cuttlefish against radical mediated lipid and DNA damage. LWT-Food Science and Technology, 64(2), 593–601. https://doi.org/10.1016/j.lwt.2015.06.031.

[15] Aubourg, S. P., Torres‐Arreola, W., Trigo, M., and Ezquerra‐Brauer, J. M., 2016. Partial characterization of jumbo squid skin pigment extract and its antioxidant potential in a marine oil system. European Journal of Lipid Science and Technology, 118(9), 1293–1304. https://doi.org/10.1002/ejlt.201500356.

[16] Li, F., Luo, P., and Liu, H., 2018. A potential adjuvant agent of chemotherapy: sepia ink polysaccharides. Marine Drugs, 16(4), 106. https://doi.org/10.3390/md16040106.

[17] Nazeer, R. A., and Naqash, S. Y., 2013. In vitro antioxidant activity of two molluscs, Loligo duvauceli Orbigny and Donax cuneatus Linnaeus, by solvent extraction methods. Mediterranean Journal of Nutrition and Metabolism, 6(1), 17–21. https://doi.org/10.1007/s12349-011-0088-1.

[18] Laemmli, U. K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685. https://doi.org/10.1038/227680a0.

[19] Ma, F., Zhao, H., Sun, L., Li, Q., Huo, L., Xia, T., ... and Feng, S., 2012. A facile route for nitrogen-doped hollow graphitic carbon spheres with superior performance in supercapacitors. Journal of Materials Chemistry, 22(27), 13464–13468. https://doi.org/10.1039/C2JM32960C.

[20] Jin, X., 2012. Bioactivities of water-soluble polysaccharides from fruit shell of Camellia oleifera Abel: Antitumor and antioxidant activities. Carbohydrate Polymers, 87(3), 2198–2201. https://doi.org/10.1016/j.carbpol.2011.10.047.

[21] Barth, A., 2007. Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1767(9), 1073–1101. https://doi.org/10.1016/j.bbabio.2007.06.004.

[22] Pappas, A., 1995. Extraction and characterization of proteases from squid processing waste (Doctoral dissertation, M. Sc. Thesis, Department of Food Science and Nutrition, University of Rhode Island, Kingston, RI).

[23] Kumar, P., Kannan, M., ArunPrasanna, V., Vaseeharan, B., and Vijayakumar, S., 2018. Proteomics analysis of crude squid ink isolated from Sepia esculenta for their antimicrobial, antibiofilm and cytotoxic properties. Microbial Pathogenesis, 116, 345–350. https://doi.org/10.1016/j.micpath.2018.01.039.

[24] Barwinvino, A., 2010. Bioactive compounds from cephalopod mollusk: Isolation, characterization and in vitro antioxidant activity of glycosa-minoglycans from cuttlefish Sepia brevimana Steenstrup, 1875 and its cardioprotective effect on isoproterenol-induced myocardial infarction in male wistar rats (Doctoral dissertation, PhD thesis, Annamalai University, Tamil Nadu, India).

[25] Nicy, B., 2016. Antioxidant And Antibacterial Properties Of Cuttlefish Ink Collected From Selected Cuttlefish Landed At Thoothukudi Coast. (Doctoral dissertation, Thesis of Master Degree).

[26] Prota, G., 2012. Melanins and melanogenesis. Academic Press.

[27] Katritzky, A. R., Akhmedov, N. G., Denisenko, S. N., and Denisko, O. V., 2002. 1H NMR spectroscopic characterization of solutions of Sepia melanin, Sepia melanin free acid and human hair melanin. Pigment cell research, 15(2), 93–97. https://doi.org/10.1034/j.1600-0749.2002.1o062.x.

[28] Chen, S. G., Xue, C. H., Xue, Y., Li, Z. J., Gao, X., and Ma, Q., 2007. Studies on the free radical scavenging activities of melanin from squid ink. Chinese Journal of Marine Drugs, 26(1), 24.

[29] Meyskens Jr, F. L., Farmer, P., and Fruehauf, J. P., 2001. Redox regulation in human melanocytes and melanoma. Pigment Cell Research, 14(3), 148–154. https://doi.org/10.1034/j.1600-0749.2001.140303.x.

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Published

11-04-2021

How to Cite

Anh, N. P., Ky, P. X., Ha, D. V., Hy, L. H. K., Thiet, D. T., & Vy, P. B. (2021). Antioxidant activity of extract from some squid species in Khanh Hoa. Vietnam Journal of Marine Science and Technology, 20(4A), 187–198. Retrieved from https://vjs.ac.vn/index.php/jmst/article/view/15660

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