PRELIMINARY STUDY ON CHEMICAL MECHANISM OF DECREASE OF TETRODOTOXIN CONTENT IN OVARIES OF A PUFFER FISH Lagocephalus inermis (Temminck and Schlegel, 1850) FERMENTED WITH RICE BRAN

Le Ho Khanh Hy; Pham Xuan Ky; Dao Viet Ha; Nguyen Thu Hong; Phan Bao Vy; Doan Thi Thiet; Nguyen Phuong Anh

doi:10.15625/1859-3097/19/1/10742

Author affiliations

Authors

Le Ho Khanh Hy Institute of Oceanography, VAST, Vietnam
Pham Xuan Ky Institute of Oceanography, VAST, Vietnam
Dao Viet Ha Institute of Oceanography, VAST, Vietnam
Nguyen Thu Hong Institute of Oceanography, VAST, Vietnam
Phan Bao Vy Institute of Oceanography, VAST, Vietnam
Doan Thi Thiet Institute of Oceanography, VAST, Vietnam
Nguyen Phuong Anh Institute of Oceanography, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/19/1/10742

Keywords:

4, 9-anhydrotetrodotoxin, fermentation, Lagocephalus inermis, ovaries, rice bran, tetrodotoxin.

Abstract

Tetrodotoxins in salted and fermented ovaries with rice bran of a puffer fish Lagocephalus inermis (Temminck & Schlegel, 1850) during five months were determined by high-performance liquid chromatography with fluorometric detector (HPLC- FLD). The analysed results showed that the original ovaries contained only tetrodotoxin with content of 7.59 µg/g fresh weight. 4,9-Anhydrotetrodotoxin - an isomer which is less toxic than tetrodotoxin has been found in all ovary samples since the second month. Tetrodotoxin in the ovaries disappeared in the 4^thmonth of the experiment. In addition, 4,9-anhydrotetrodotoxin level in ovaries increased during fermentation. The present study contributes to clarifying the chemical mechanism of decreasing tetrodotoxin content in fermented ovary of puffer fish.

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