EFFECTS OF DIETARY MANNAN OLIGOSACCHARIDE ON MUSCLE PROTEIN, INTESTINAL MORPHOLOGY AND BLOOD CELL COUNT OF CLOWNFISH, AMPHIPRION OCELLARIS
Keywords:Clownfish, Amphiprion ocellaris, mannan oligosaccharide, gut morphology, protein, blood cell.
AbstractClownfish, Amphiprion ocellaris (2.4 cm) were cultured in recirculation system, volume of 50 L and density of 20 fish per tank. Six levels of mannan oligosacharide (MOS), including 0.05%, 0.10%, 0.15%, 0.20%, 0.25% and 0.30% were supplemented to the control diet and fed to the fishfor 10 weeks. Collected data included ratio of internal and external intestinal perimeter of the gut (PR), muscle protein content and total blood cell count. Result showed that the PR was highest in the fish fed by 0.1 and 0.15% MOS and lowest in two groups of fish fed by control and 0.3% MOS in their diet (P <0.05). After 4 weeks of diet feeding, protein content in the fish among the treatments ranged from 12.85%P to 15.53%P. Those values seemed to increase at week 10 in compared to week 4, however; there was no significant difference in protein content among the treatments at either week 4 or week 10 measurements. Total blood cell count of fish at week 10 were highest in the group of fish fed on 0.05% MOS (26.22×105 ± 2.80×105 cells/mm3), followed by the groups of fish fed 0.25% and 0.1% with blood count being 21.54×105 ± 3.65×105 cells/mm3 and 20.73×105 ± 7.88 ×105 cells/mm3. Respectively the lowest blood cell count was in the fish fed by 0.15% MOS MOS (11,02×105 ± 2,00 ×105 cells /mm3). There was a statistically significant difference between blood cell count of fish fed by 0.05% MOS in compared to fish fed by control diet and 0.15% MOS (P < 0,05).
Akrami, R., Chitsaz, H., Hezarjaribi, A. and Ziaei, R., 2012a. Effect of Dietary Mannan Oligosaccharide (MOS) on Growth Performance and Immune Response of Gibel Carp Juveniles (Carassius auratus gibelio). J Vet Adv. 2, 507-513.
Akrami, R., Mansour, M. R., Chitsaz, H., Ziaei, R. and Ahmadi, Z., 2012b. Effect of Dietary Mannan Oligosaccharide on Growth Performance, Survival, Body Composition and Some Hematological Parameters of Carp Juvenile (Cyprinus Carpio). J. Anim. Sci. Adv. 2, 879-885.
Akrami, R., Razeghi Mansour, M., Ghobadi, S., Ahmadifar, E., Shaker Khoshroudi, M. and Moghimi Haji, M.S., 2013. Effect of prebiotic mannan oligosaccharide on hematological and blood serum biochemical parameters of cultured juvenile great sturgeon (Huso huso Linnaeus, 1754). Journal of Applied Ichthyology, doi: 10.1111/jai.12245.
Daniels, C. L., Merrifield, D. L., Boothroyd, D. P., Davies, S. J., Factor, J. R. and Arnold, K. E., 2010. Effect of dietary Bacillus spp. and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance, gut morphology and gut microbiota. Aquaculture. 304, 49-57.
Dimitroglou, A., Davies, S. and Sweetman, J., 2008. The effect of dietary mannan oligosaccharides on the intestinal histology of rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology, Part A. 150, Abstract.
Dimitroglou, A., Moate, R., Janssens, T., Spring, P., Sweetman, J. W. and Davies, S. J., 2011a. Field Observations on the Effect of a Mannan Oligosaccharide on Mortality and Intestinal Integrity of Sole (Solea senegalensis, Kaup) Infected by Photobacterium damselae subsp. piscicida. J Aquac Res Development. S1, 013.
Dimitroglou, A., Reynolds, P., Ravnoy, B., Johnsen, F., Sweetman, J. W., Johansen, J. and Davies, S. J., 2011b. The effect of Mannan oligosaccharide supplementation on Atlantic salmon Smolts (Samo salar L.) Fed diet with high levels of plant proteins. Aquaculture Research & Development. S1, 11 p.
Genc, M. A., Aktas, M., Genc, E. and Yilmaz, E., 2007. Effects of dietary mannan oligosaccharide on growth, body composition and hepatopancreas histology of Penaeus semisulcatus (de Haan 1844). Aquaculture Nutrition. 13, 156-161.
Gultepe, N., Salnur, S., HoŞSu, B. and Hisar, O., 2011. Dietary supplementation with Mannanoligosaccharides (MOS) from Bio-Mos enhances growth parameters and digestive capacity of gilthead sea bream (Sparus aurata). Aquaculture Nutrition. 17, 482-487.
Hai, N. V. and Fotedar, R., 2009. Comparison of the effects of the prebiotics (Bio-Mos® and β-1,3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P. aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye, 1896). Aquaculture. 289, 310-316.
Jagadeesh, T. D., Rather, M. A., Chethan, N., Divya, K. H. V. and Suresh, A. N., 2013. Effect of dietary supplementation of mannan oligosaccharide on water quality parameters in a recirculatory aquaculture system. European Journal of Experimental Biology. 3, 48-55
Nekoubin, H., Gharedaashi, E., Imanpour, M. R., Nowferesti, H. and Asgharimoghadam, A., 2012. The Influence of Synbiotic (Biomin imbo) on Growth Factors and Survival Rate of Zebrafish (Danio rerio) Larvae via Supplementation with Biomar. Global Veterinaria. 8, 503-506.
Peterson, B. C., Bramble, T. C. and Manning, B. B., 2010. Effects of Bio-Mos® on Growth and Survival of Channel Catfish Challenged with Edwardsiella ictaluri. Journal of the World Aquaculture Society. 41, 149-155.
Sang, H. M. and Fotedar, R., 2010. Prebiotic Mannan Oligosaccharide Diet Improves Health Status of the Digestive System of Marron, Cherax tenuimanus (Smith 1912). Journal of Applied Aquaculture. 22, 240-250.
Zhang, J., Liu, Y., Tian, L., Yang, H., Liang, G. and Xu, D., 2012. Effects of dietary mannan oligosaccharide on growth performance, gut morphology and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology. 33, 1,027-1,032