Bacteria associated with soft coral from Mot island - Nha Trang bay and their antimicrobial activities
Keywords:Bacillus sp., antimicrobial activity, soft coral associated bacteria.
AbstractMicrobial communities associated with invertebrates had been considered as a new source of bioactive compounds. The soft coral associated bacteria in Mot island, Nha Trang bay were isolated, extracted and assessed for antagonistic activity against human and coral pathogens, the strongly active strains were identified by 16S rRNA analysis. The soft coral associated bacterium SCN10 had abcd antibacterial pattern which was named for inhibition towards Bacillus subtilis (pattern a), Escherichia coli (pattern b), Salmonella typhimurium (pattern c) and Serratia marcescens (pattern d). It was the nearest strain to the well-known antibiotic producer Bacillus amyloliquefaciens with 99% sequence similarity. Whereas strain SCL19 had abde pattern which means inhibition of the growth of B. subtilis, E. coli, S. marcescens and Vibrio parahaemolyticus (pattern e). This strain SCL19 affiliated with Bacillus sp. strain A-3-23B with 99.8% identity. In addition to antimicrobial activity to the aforementioned tested bacteria, the isolate SCX15 also inhibited Vibrio alginolyticus (pattern f) and Candida albicans (pattern g), so this isolate possessed abcdefg antimicrobial pattern. The coral associated isolate SCX15 was identified as Bacillus velezensis with 99% sequence similarity. Among the 78 screened strains, 25 isolates possessed antibacterial activity against at least one of seven tested microorganisms and exhibited 12 different types of antimicrobial activities, suggesting that they can produce many different natural substances with antibacterial activity.
Rosenberg, E., Kushmaro, A., Kramarsky-Winter, E., Banin, E., and Yossi, L., 2009. The role of microorganisms in coral bleaching. The ISME Journal, 3(2), 139–146.
Imhoff, J. F., Labes, A., and Wiese, J., 2011. Bio-mining the microbial treasures of the ocean: new natural products. Biotechnology Advances, 29(5), 468–482.
Pedrós-Alió, C., 2006. Marine microbial diversity: can it be determined?. Trends in Microbiology, 14(6), 257–263.
Lampert, Y., Kelman, D., Dubinsky, Z., Nitzan, Y., and Hill, R. T., 2006. Diversity of culturable bacteria in the mucus of the Red Sea coral Fungia scutaria. FEMS Microbiology Ecology, 58(1), 99–108.
Neulinger, S. C., Järnegren, J., Ludvigsen, M., Lochte, K., and Dullo, W. C., 2008. Phenotype-specific bacterial communities in the cold-water coral Lophelia pertusa (Scleractinia) and their implications for the coral’s nutrition, health, and distribution. Appl. Environ. Microbiol., 74(23), 7272–7285.
Shnit-Orland, M., and Kushmaro, A., 2009. Coral mucus-associated bacteria: a possible first line of defense. FEMS Microbiology Ecology, 67(3), 371–380.
Zhang, X., Sun, Y., Bao, J., He, F., Xu, X., and Qi, S., 2012. Phylogenetic survey and antimicrobial activity of culturable microorganisms associated with the South China Sea black coral Antipathes dichotoma. FEMS Microbiology Letters, 336(2), 122–130.
Peng, J., Zhang, X., Xu, X., He, F., and Qi, S., 2013. Diversity and chemical defense role of culturable non-actinobacterial bacteria isolated from the South China Sea gorgonians. J. Microbiol Biotechnol, 23(4), 437–43.
Kvennefors, E. C. E., Sampayo, E., Kerr, C., Vieira, G., Roff, G., and Barnes, A. C., 2012. Regulation of bacterial communities through antimicrobial activity by the coral holobiont. Microbial Ecology, 63(3), 605–618.
Nithyanand, P., and Pandian, S. K., 2009. Phylogenetic characterization of culturable bacterial diversity associated with the mucus and tissue of the coral Acropora digitifera from the Gulf of Mannar. FEMS Microbiology Ecology, 69(3), 384–394.
Nissimov, J., Rosenberg, E., and Munn, C. B., 2009. Antimicrobial properties of resident coral mucus bacteria of Oculina patagonica. FEMS Microbiology Letters, 292(2), 210–215.
Rocha, J., Peixe, L., Gomes, N., and Calado, R., 2011. Cnidarians as a source of new marine bioactive compounds—An overview of the last decade and future steps for bioprospecting. Marine Drugs, 9(10), 1860–1886.
Tung, N. H., Van Minh, C., Van Kiem, P., Huong, H. T., Nam, N. H., Cuong, N. X., ... and Kim, Y. H., 2010. Chemical components from the Vietnamese soft coral Lobophytum sp. Archives of Pharmacal Research, 33(4), 503–508.
Quang, T. H., Ha, T. T., Van Minh, C., Van Kiem, P., Huong, H. T., Ngan, N. T. T., ... and Song, S. B., 2011. Cytotoxic and anti-inflammatory cembranoids from the Vietnamese soft coral Lobophytum laevigatum. Bioorganic & Medicinal Chemistry, 19(8), 2625–2632.
Quang, T. H., Ha, T. T., Van Minh, C., Van Kiem, P., Huong, H. T., Ngan, N. T. T., ... and Song, S. B., 2011. Cytotoxic and PPARs transcriptional activities of sterols from the Vietnamese soft coral Lobophytum laevigatum. Bioorganic & Medicinal Chemistry Letters, 21(10), 2845–2849.
Thao, N. P., Luyen, B. T. T., Ngan, N. T. T., Song, S. B., Cuong, N. X., Nam, N. H.,... and Van Minh, C., 2014. New anti-inflammatory cembranoid diterpenoids from the Vietnamese soft coral Lobophytum crassum. Bioorganic & medicinal chemistry letters, 24(1), 228–232.
Thao, N. P., Luyen, B. T. T., Sun, Y. N., Song, S. B., Van Thanh, N., Cuong, N. X., ... and Van Minh, C., 2014. NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. Bioorganic & Medicinal Chemistry Letters, 24(13), 2834–2838.
Thao, N. P., Nam, N. H., Cuong, N. X., Quang, T. H., Tung, P. T., Chae, D., ... and Kim, Y. H., 2013. Anti-inflammatory norditerpenoids from the soft coral Sinularia maxima. Bioorganic & Medicinal Chemistry Letters, 23(1), 228–231.
Mien, P. T., Thang, N. N., and Hanh, N. K., 2018. Bacillus sp. VK2 isolated from Acropora hyacinthus from Ninh Thuan and its antimicrobial activities against cause of white pox disease in Acropora palmate. Vietnam Journal of Marine Science and Technology, 18(2), 197–204.
Mincer, T. J., Jensen, P. R., Kauffman, C. A., and Fenical, W., 2002. Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl. Environ. Microbiol., 68(10), 5005–5011.
Bauer, A. W., Kirby, W. M., Sherris, J. C., and Turck, M., 1966. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45(4), 493–496.
Halebian, S., Harris, B., Finegold, S. M., and Rolfe, R. D., 1981. Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. Journal of Clinical Microbiology, 13(3), 444–448.
Pham, T. M., Wiese, J., Wenzel-Storjohann, A., and Imhoff, J. F., 2016. Diversity and antimicrobial potential of bacterial isolates associated with the soft coral Alcyonium digitatum from the Baltic Sea. Antonie Van Leeuwenhoek, 109(1), 105–119.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J., 1990. Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410.
Zhang, H., Lee, Y. K., Zhang, W., and Lee, H. K., 2006. Culturable actinobacteria from the marine sponge Hymeniacidon perleve: isolation and phylogenetic diversity by 16S rRNA gene-RFLP analysis. Antonie Van Leeuwenhoek, 90(2), 159–169.
Taylor, M. W., Radax, R., Steger, D., and Wagner, M., 2007. Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. Microbiol. Mol. Biol. Rev., 71(2), 295–347.
Mien, P. T., Ha, D. V., and Hanh, N. K., 2017. Antibiotic resistances of marine bacteria from Hon Mot, Nha Trang bay. Vietnam Journal of Marine Science and Technology, 17(4), 480–489.
Simon, C., and Daniel, R., 2011. Metagenomic analyses: past and future trends. Appl. Environ. Microbiol., 77(4), 1153–1161.
Patterson, K. L., Porter, J. W., Ritchie, K. B., Polson, S. W., Mueller, E., Peters, E. C., ... and Smith, G. W., 2002. The etiology of white pox, a lethal disease of the Caribbean elkhorn coral, Acropora palmata. Proceedings of the National Academy of Sciences, 99(13), 8725–8730.
Nguyen, K. H., Pham, T. M., Bui, H. H., Vo, H. T., 2016. Screening of coral associated bacteria with antimicrobial activities from scleractinian coral Acropora muricata in the Nha Trang bay. Collection of Marine Research Works, 22, 83–95. (in Vietnamese).
Mien, P. T., and Van Khoa, N., 2019. Screening of mangrove-derived bacteria from Ninh Ich, Ninh Hoa, Khanh Hoa for antimicrobial producers. Vietnam Journal of Marine Science and Technology, 19(4), 601–610.
Martínez-Luis, S., Ballesteros, J., and Gutiérrez, M., 2011. Antibacterial constituents from the octocoral-associated bacterium Pseudoalteromonas sp. Revista Latinoamericana de Química, 39(1–2), 75–83.
Gao, C. H., Tian, X. P., Qi, S. H., Luo, X. M., Wang, P., and Zhang, S., 2010. Antibacterial and antilarval compounds from marine gorgonian-associated bacterium Bacillus amyloliquefaciens SCSIO 00856. The Journal of Antibiotics, 63(4), 191–193.
Rabbee, M. F., Ali, M., Choi, J., Hwang, B. S., Jeong, S. C., and Baek, K. H., 2019. Bacillus velezensis: a valuable member of bioactive molecules within plant microbiomes. Molecules, 24(6), 1046.
Chakraborty, K., Thilakan, B., and Raola, V. K., 2014. Polyketide Family of Novel Antibacterial 7-O-Methyl-5′-hydroxy-3′-heptenoate-Macrolactin from Seaweed-Associated Bacillus subtilis MTCC 10403. Journal of Agricultural and Food Chemistry, 62(50), 12194-12208.
Chakraborty, K., Thilakan, B., and Raola, V. K., 2018. Previously undescribed antibacterial polyketides from heterotrophic Bacillus amyloliquefaciens associated with seaweed Padina gymnospora. Applied Biochemistry and Biotechnology, 184(2), 716–732.
Caulier, S., Nannan, C., Gillis, A., Licciardi, F., Bragard, C., and Mahillon, J., 2019. Overview of the antimicrobial compounds produced by members of the Bacillus subtilis group. Frontiers in microbiology, 10, 302–321.
Pham, T. M., 2014. Community of soft coral Alcyonium digitatum associated bacteria and their antimicrobial activities. Doctoral dissertation, Christian-Albrechts Universität Kiel.
Wiese, J., Abdelmohsen, U. R., Motiei, A., Humeida, U. H., and Imhoff, J. F., 2018. Bacicyclin, a new antibacterial cyclic hexapeptide from Bacillus sp. strain BC028 isolated from Mytilus edulis. Bioorganic & Medicinal Chemistry Letters, 28(4), 558–561.