• Pham Xuan Ky Institute of Oceanography, VAST, Vietnam
  • Phan Bao Vy Institute of Oceanography, VAST, Vietnam
  • Dao Viet Ha Institute of Oceanography, VAST, Vietnam
  • Le Ho Khanh Hy Institute of Oceanography, VAST, Vietnam
  • Nguyen Thu Hong Institute of Oceanography, VAST, Vietnam
  • Doan Thi Thiet Institute of Oceanography, VAST, Vietnam
  • Nguyen Phuong Anh Institute of Oceanography, VAST, Vietnam



ASC, PSC, hydrolyzed collagen, enzymes, SDS-PAGE, antioxidant activity, fan-bellied leatherjacket.


Collagen extracted from the skin of fan-bellied leatherjacket was hydrolyzed and tested for antioxidant activity. The yields of acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were 14.8% and 19.6%, respectively, based on the wet weight of skin. The SDS-PAGE results showed that collagen from the skin of this fish consists of α1 and α2 chains with molecular weight (MW) approximately 100 kDa, identified as type I collagen. Hydrolysis of collagen by the other enzymes, such as papain, bromelain, pepsin and alcalase could produce the peptides with MW less than 28 kDa. Hydrolyzed collagen possessed the antioxidant activity with different levels and greater than that of normal collagen.


Download data is not yet available.


Aberoumand, A., 2012. Comparative study between different methods of collagen extraction from fish and its properties. World Applied Sciences Journal, 16(3), 316–319.

Zeugolis, D., Khew, S., Yew, E., Ekaputra, A., Tong, Y., Yung, L., Hutmacher, D., Sheppard, C. and Raghunath, M., 2008. Electro-spinning of pure collagen nano-fibres - Just an expensive way to make gelatin?. Biomaterials, 29(15), 2293–2305.

Kim, J. and Park, J., 2004. Characterization of acid-soluble collagen from Pacific whiting surimi processing byproducts. Journal of Food Science, 69(8), 637–642.

Whitehurst, E., and Oort, E., 2009. Enzymes in Food Technology, 2nd Edition. John Wiley & Sons.

Yang, H., Wang, H., Zhao, Y., Wang, H. and Zhang, H., 2014. Effect of heat treatment on the enzymatic stability of grass carp skin collagen and its ability to form fibrils in vitro. Journal of the Science of Food and Agriculture, 95(2), 329–336.

Silva, T., Moreira-Silva, J., Marques, A., Domingues, A., Bayon, Y. and Reis, R., 2014. Marine origin collagens and its potential applications. Marine Drugs, 12(12), 5881–5901.

Mahboob, S., 2014. Isolation and characterization of collagen from fish waste material-skin, scales and fins of Catla catla and Cirrhinus mrigala. Journal of Food Science and Technology, 52(7), 4296–4305.

Wang, B., Wang, Y., Chi, C., Luo, H., Deng, S. and Ma, J., 2013. Isolation and Characterization of collagen and antioxidant collagen peptides from scales of Croceine Croaker (Pseudosciaena crocea). Marine Drugs, 11(11), 4641–4661.

Nagai, T. and Suzuki, N., 2000. Isolation of collagen from fish waste material-skin, bone and fins. Food Chemistry, 68, 277–281.

Laemmli, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature, 227, 680–685.

Hema, G. S., Joshy, C. G., Shyni, K., Chatterjee, N. S., Ninan, G and Mathew, S., 2017. Optimization of process parameters for the production of collagen peptides from fish skin (Epinephelus malabaricus) using response surface methodology and its characterization. Journal of Food Science and Technology, 54(2), 488–496.

Chi, C. F., Cao, Z. H., Bin, B., Hu, F. Y., Li, Z. R., and Zhang, B., 2014. Antioxidant and functional properties of collagen hydrolysates from Spanish mackerel skin as influenced by average molecular weight. Molecules, 19, 11211–11230.

Baehaki, A., Nopianti, R., and Anggraeni, S., 2015. Antioxidant activity of skin and bone collagen hydrolyzed from striped catfish (Pangasius pangasius) with papain enzyme. Journal of Chemical and Pharmaceutical Research, 7(11), 131–135.

Jamilah, B., Umi Hartina, M. R., Mat Hashim, D., and Sazili, A. Q., 2013. Properties of collagen from barramundi (Lates calcarifer) skin. International Food Research Journal, 20(2), 835–842.

Yang, L., Wu, X., Feng, W., Zhao, T., Zhou, Y., Li, F., Zou, Y., Bai, S., and Wang, W., 2013. Optimization of enzyme-assisted extraction and characterization of collagen from Chinese sturgeon (Acipenser sturio Linnaeus) skin. Pharmacognosy Magazine, 9(36), 32–37.

Muralidharan, N., Jeya Shakila, R., Sukumar, D., and Jeyasekaran, G., 2011. Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization. Journal of Food Science and Technology, 50(6), 1106–1113.

Nalinanon, S., Benjakul, S., and Kishimura, H., 2010. Collagens from the skin of arabesque greenling (Pleurogrammus azonus) solubilized with the aid of acetic acid and pepsin from albacore tuna (Thunnus alalunga) stomach. Journal of the Science of Food and Agriculture, 90(9), 1492–1500.

Kiew, P. L., Mat Don, M., 2013. The influence of acetic acid concentration on the extractability of collagen from the skin of hybrid Clarias sp. and its physicochemical properties: a preliminary study. Focusing on Modern Food Industry (FMFI), 2, 123–128.

Khiari, Z., Ndagijimana, M., and Betti, M., 2014. Low molecular weight bioactive peptides derived from the enzymatic hydrolysis of collagen after isoelectric solubilization/precipitation process of turkey by-products. Poultry Science, 93(9), 2347–2362.

Liua, F., Liub, C. E., Lorenac, D., and Zhang X., 2012. Evaluation of the antioxidant activity of collagen peptide additive extracted from Cod skin. Journal of Environmental Protection and Ecology, 13(3), 1836–1841.