INVESTIGATION OF THE LIPID, PHOSPHOLIPID, FATTY ACID AND PHOSPHATIDYLCHOLINE CONTENT AND COMPOSITION OF THE VIETNAMESE SOFT CORAL CAPNELLA SP.
Keywords:Total lipid (TL), polar lipid (PoL), non-polar lipid (NL), free fatty acid (FFA), sterol (ST), triacylglycerol (TAG), monoankyldiacylglycerol (MADAG), hydrocarbon - wax (WE), phosphatidylcholine (PC).
AbstractThe total lipid content of the soft coral Capnella sp. contained 1.44 ± 0.10% of the fresh weight. In the fatty acid content, the tetracosapolyenoic fatty acids 24:5n-6 and 24:6n-3, which were the typical markers for the soft corals (the eight compartment coral), and the fatty acids that marked for symbiotic microorganism including 18:5n-3, 18:2n-6, 18:3n-6, 18:4n-3, 20:4n-3 were presented. The result of the lipid composition analysis showed that the total lipid contained polar lipid, sterol, free fatty acid, triacylglycerol, monoalkyldiacylglycerol, hydrocarbon and wax. Of these, the polar lipid displayed the highest content with 21.14 ± 1.17% of the total lipid. The phospholipid composition of the soft coral Capnella sp. contained the characteristic phospholipid subclasses of the animals of the phylum Cnidaria, including phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, and ceramide aminoethylphosphonate (phosphonolipid, CAEP). Of these, phosphatidylcholine exhibited the highest content with 35.53 ± 1.46% of the total phospholipid. This is the first time that the content and composition of the molecular types of the phosphatidylcholine from a Vietnamese soft coral species have been investigated and reported. By using modern mass spectrometry IT TOF LC-MS, the presence of 13 molecular types have been precisely identified, which PC 18:0e/20:4 and PC 16:0e/20:4 were the two ingredients displaying the highest content.
Phạm Quốc Long, Imbs Andrey Borisovich, 2012. Lipit, axit béo và oxylipin của san hô. Nxb. Khoa học Tự nhiên và Công nghệ. Tr. 125-184.
Folch, J., Lees, M., and Stanley, G. S., 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry, 226(1): 497-509.
Phattanawasin, P., Sotanaphun, U., Sriphong, L., Kanchanphibool, I., and Piyapolrungroj, N., 2011. A comparison of image analysis software for quantitative TLC of ceftriaxone sodium. Silpakorn University Science & Technology Journal, 5(1): 7-13.
Svetashev, V. T., and Vaskovsky, V. E., 1972. A simplified technique for thin-layer microchromatography of lipids. Journal of Chromatography A, 67(2): 376-378.
Vaskovsky, V. E., and Terekhova, T. A., 1979. HPTLC of phospholipid mixtures containing phosphatidylglycerol. Journal of High Resolution Chromatography, 2(11): 671-672.
Kostetsky, E. Y., 1984. The phospholipid-composition of Spongia, Coelenterata, Plathelminthes, Nemertini, Annelida, Sipunculida and Echiurida. Biologiya Morya-Marine Biology, (5), 46-53.
Imbs, A. B., Dang, L. P., Rybin, V. G., and Svetashev, V. I., 2015. Fatty acid, lipid class, and phospholipid molecular species composition of the soft coral Xenia sp. (Nha Trang bay, the South China Sea, Vietnam). Lipids, 50(6): 575-589.
Imbs, A. B., Maliotin, A. N., Lưu Văn Huyền, Phạm Quốc Long, 2005. Nghiên cứu thành phần axit béo của 17 loài san hô Việt Nam. Tạp chí Khoa học và Công nghệ, 43, 84-91.
Lưu Văn Huyền, 2010. Nghiên cứu lipid và axit béo của một số loài san hô biển Việt Nam. Luận án Tiến sỹ. Viện Hóa học Các hợp chất thiên nhiên. Tr. 63-64.
Yamashiro, H., Oku, H., Higa, H., Chinen, I., and Sakai, K., 1999. Composition of lipids, fatty acids and sterols in Okinawan corals. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 122(4): 397-407.
Hamoutene, D., Puestow, T., Miller-Banoub, J., and Wareham, V., 2008. Main lipid classes in some species of deep-sea corals in the Newfoundland and Labrador region (Northwest Atlantic Ocean). Coral reefs, 27(1): 237-246.
Nichols, P. D., Danaher, K. T., and Koslow, J. A., 2003. Occurrence of high levels of tetracosahexaenoic acid in the jellyfish Aurelia sp.. Lipids, 38(11): 1207-1210.
Imbs, A. B., Demidkova, D. A., Dautova, T. N., and Latyshev, N. A., 2009. Fatty acid biomarkers of symbionts and unusual inhibition of tetracosapolyenoic acid biosynthesis in corals (Octocorallia). Lipids, 44(4): 325-335.
Latyshev, N. A., Nguen, K. H., To, T. N., and Svetashev, V. I., 1986. Composition and seasonal fluctuations of alcyonarian phospholipids. Russian Journal of Marine Biology, 12, 178-182.
de Souza, L. M., Iacomini, M., Gorin, P. A., Sari, R. S., Haddad, M. A., and Sassaki, G. L., 2007. Glyco-and sphingophosphonolipids from the medusa Phyllorhiza punctata: NMR and ESI-MS/MS fingerprints. Chemistry and physics of lipids, 145(2): 85-96.
Mukhamedova, K. S., and Glushenkova, A. I., 2000. Natural phosphonolipids. Chemistry of Natural Compounds, 36(4): 329-341.