Antimicrobial activity assessment of crude broth fermented extracts of marine-derived fungi collected from Bai Tu Long Bay (North Vietnam)

Nguyen Thi Hoang Anh Nguyen Thi Hoang Anh; Thi Thu Huyen Vu; Mai Anh Nguyen; Thi Mai Huong Doan; Van Cuong Pham; Thanh Xuan Dam; Duc Tuan Cao; Thi Hong Minh Le

doi:10.15625/1859-3097/18686

Author affiliations

Authors

Nguyen Thi Hoang Anh Institute of Marine Biochemistry, VAST, Vietnam; Thai Binh Medical College, Thai Binh, Vi https://orcid.org/0000-0002-3211-5254
Vu Thi Thu Huyen Institute of Marine Biochemistry, VAST, Vietnam
Nguyen Mai Anh Institute of Marine Biochemistry, VAST, Vietnam
Doan Thi Mai Huong Institute of Marine Biochemistry, VAST, Vietnam
Pham Van Cuong Institute of Marine Biochemistry, VAST, Vietnam
Dam Thanh Xuan Hanoi University of Pharmacy, Hanoi, Vietnam
Cao Duc Tuan Hai Phong University of Medicine and Pharmacy, Hai Phong, Vietnam
Le Thi Hong Minh Institute of Marine Biochemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/18686

Keywords:

Aspergillus, Antimicrobial activity, MIC, Marine fungi, Hamigera avellanea.

Abstract

This study aimed to isolate and identify marine fungi from Bai Tu Long Bay and assess their antimicrobial potential. We successfully isolated twenty strains of marine-derived fungi. The crude extracts from these fungi were tested against pathogenic microorganisms. All twenty strains exhibit some degree of growth inhibition against the tested microorganisms. Notably, strains M223, M250, M253, and M256 showed significant antimicrobial activity, with MIC values equal to or lower than the positive control. These results highlight the potential of marine fungi as a rich source of antimicrobial agents, a finding of considerable importance to marine mycology and pharmaceuticals. Further analysis was conducted on four promising isolates. M253 was identified as Hamigera avellanea, while M223, M250, and M256 were found to belong to the Aspergillus genus. These isolates were then analyzed using a phylogenetic tree based on MegaX software.

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References

Beygmoradi, A., and Homaei, A., 2017. Marine microbes as a valuable resource for brand new industrial biocatalysts. Biocatalysis and Agricultural Biotechnology, 11, 131–152.

Gonçalves, M. F., Esteves, A. C., and Alves, A., 2022. Marine fungi: Opportunities and challenges. Encyclopedia, 2(1), 559–577.

Bills, G. F., and Gloer, J. B., 2016. Biologically active secondary metabolites from the fungi. Microbiology spectrum, 4(6), 10–1128.

Le Thi, H. M., Nguyen, M. A., Vu, T. Q., Phi , T. D., Tran , V. H., Doan Thi, M. H., Pham, V. C., and Vu Thi, T. H., 2023. Antibacterial activities and characteristics of some marine fungi strains isolated from Co To beach, Quang Ninh Province. Vietnam Journal of Marine Science and Technology, 23(3), 311–320.

Hasan, S., Ansari, M. I., Ahmad, A., and Mishra, M., 2015. Major bioactive metabolites from marine fungi: A Review. Bioinformation, 11(4), 176–181.

Machado, F. P., Kumla, D., Pereira, J. A., Sousa, E., Dethoup, T., Freitas-Silva, J., Costa, P. M., Mistry, S., Silva, A. M. S., and Kijjoa, A., 2021. Prenylated phenylbutyrolactones from cultures of a marine sponge-associated fungus Aspergillus flavipes KUFA1152. Phytochemistry, 185, 112709.

Hadacek, F., and Greger, H., 2000. Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 11(3), 137–147.

Xu, X., Guo, S., Chen, H., Zhang, Z., Li, X., Wang, W., and Guo, L., 2021. Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam. 3 Biotech, 11(4), 193.

Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547.

Nei, M., and Gojobori, T., 1986. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Molecular biology and evolution, 3(5), 418–426.

Cen, S., Jia, J., Ge, Y., Ma, Y., Li, X., Wei, J., Bai, Y., Wu, X., Song, J., Bi, H., and Wu, B., 2021. A new antibacterial 3, 5-dimethylorsellinic acid-based meroterpene from the marine fungus Aspergillus sp. CSYZ-1. Fitoterapia, 152, 104908.

Isaka, M., Chinthanom, P., Kongthong, S., Supothina, S., and Ittiworapong, P., 2010. Hamigeromycins C–G, 14-membered macrolides from the fungus Hamigera avellanea BCC 17816. Tetrahedron, 66(4), 955–961.

Klaram, R., Dethoup, T., Machado, F. P., Gales, L., Kumla, D., Hafez Ghoran, S., Sousa, E., Mistry, S., Silva, A. M. S., and Kijjoa, A., 2023. Pentaketides and 5-p-hydroxyphenyl-2-pyridone derivative from the culture extract of a marine sponge-associated fungus Hamigera avellanea KUFA0732. Marine Drugs, 21(6), 344.

Song, F., Lin, R., Yang, N., Jia, J., Wei, S., Han, J., Li, J., Bi, H., and Xu, X., 2021. Antibacterial secondary metabolites from marine-derived fungus Aspergillus sp. IMCASMF180035. Antibiotics, 10(4), 377.

Luo, Y., Wei, X., Yang, S., Gao, Y. H., and Luo, Z. H., 2020. Fungal diversity in deep-sea sediments from the Magellan seamounts as revealed by a metabarcoding approach targeting the ITS2 regions. Mycology, 11(3), 214–229.