Antibacterial activities and characteristics of some marine fungi strains isolated from Co To beach, Quang Ninh province

Hong Minh Le Thi; Mai Anh Nguyen; Thi Quyen Vu; Thi Dao Phi; Van Hieu Tran; Mai Huong Doan Thi; Van Cuong Pham; Thu Huyen Vu Thi

doi:10.15625/1859-3097/18252

Author affiliations

Authors

Le Thi Hong Minh Institute of Marine Biochemistry, VAST, Vietnam https://orcid.org/0000-0002-3211-5254
Nguyen Mai Anh Institute of Marine Biochemistry, VAST, Vietnam
Vu Thi Quyen Institute of Marine Biochemistry, VAST, Vietnam
Phi Thi Dao Institute of Marine Biochemistry, VAST, Vietnam
Tran Van Hieu Institute of Marine Biochemistry, VAST, Vietnam
Doan Thi Mai Huong Institute of Marine Biochemistry, VAST, Vietnam
Pham Van Cuong Institute of Marine Biochemistry, VAST, Vietnam
Vu Thi Thu Huyen Institute of Marine Biochemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/18252

Keywords:

Fungus, antimicrobial activity, bioassay, MIC, marine fungi, 18S rRNA.

Abstract

Resistance to pathogenic bacteria may lead to serious health problems. Scientists found that discovering novel antimicrobial compounds is possible by exploring rarely investigated environments. Therefore, this work focused on isolating and identifying some fungal strains collected from the Co To sea. We tested whether such strains can produce compounds with vital activities, including antibacterial and antifungal. The antimicrobial activity of the marine fungi crude extracts was performed by the Bioassay method in a 96-well tray. The minimum inhibitory concentration (MIC) test results showed that 22 strains of marine fungi from samples with different geographic coordinates and 20/22 strains had antibacterial activity against at least two strains of microorganisms tested. The biological evaluation revealed that strains M257 and M238 inhibited 4 to 5 tested strains with MIC values equal to or lower than positive controls. Using a BLAST analysis in the GenBank database, morphological comparisons of the two selected candidate strains with similar known species and phylogenetic analyses were conducted on the 18S rRNA gene regions, and maximum likelihood revealed that M257 belongs to Talaromyces genus, and M238 belongs to Aspergillus penicillioides. The isolates were analyzed in a phylogenetic tree based on MegaX software.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Amend, A., Burgaud, G., Cunliffe, M., Edgcomb, V. P., Ettinger, C. L., Gutiérrez, M. H., Heitman, J., Hom, E. F. Y., Ianiri, G., Jones, A. C., Kagami, M., Picard, K. T., Quandt, C. A., Raghukumar, S., Riquelme, M., Stajich, J., Vargas-Muñiz, J., Walker, A. K., Yarden, O., and Gladfelter, A. S., 2019. Fungi in the marine environment: Open questions and unsolved problems. MBio, 10(2), e01189–18. DOI: https://doi.org/10.1128/mBio.01189-18

Richards, T. A., Jones, M. D., Leonard, G., and Bass, D., 2012. Marine fungi: their ecology and molecular diversity. Annual Review of Marine Science, 4, 495–522. DOI: https://doi.org/10.1146/annurev-marine-120710-100802

Silber, J., Kramer, A., Labes, A., and Tasdemir, D., 2016. From discovery to production: biotechnology of marine fungi for the production of new antibiotics. Marine drugs, 14(7), 137. DOI: https://doi.org/10.3390/md14070137

Tarman, K., 2020. Marine Fungi as a Source of Natural Products. Encyclopedia of Marine Biotechnology, 4, 2147–2160. DOI: https://doi.org/10.1002/9781119143802.ch96

Queirós, B., Barreira, J. C., Sarmento, A. C., and Ferreira, I. C., 2009. In search of synergistic effects in antioxidant capacity of combined edible mushrooms. International Journal of Food Sciences and Nutrition, 60(sup6), 160–172. DOI: https://doi.org/10.1080/09637480903153845

Zeghal, E., Vaksmaa, A., Vielfaure, H., Boekhout, T., and Niemann, H., 2021. The potential role of marine fungi in plastic degradation–a review. Frontiers in Marine Science, 8, 738877. https://doi.org/10.3389/fmars.2021.738877 DOI: https://doi.org/10.3389/fmars.2021.738877

Gomes, N. G., Madureira-Carvalho, Á., Dias-da-Silva, D., Valentao, P., and Andrade, P. B., 2021. Biosynthetic versatility of marine-derived fungi on the delivery of novel antibacterial agents against priority pathogens. Biomedicine & Pharmacotherapy, 140, 111756. DOI: https://doi.org/10.1016/j.biopha.2021.111756

Carroll, A. R., Copp, B. R., Davis, R. A., Keyzers, R. A., and Prinsep, M. R., 2019. Marine natural products. Natural Product Reports, 36(1), 122–173. DOI: https://doi.org/10.1039/C8NP00092A

Papon, N., Copp, B. R., and Courdavault, V., 2022. Marine drugs: Biology, pipelines, current and future prospects for production. Biotechnology Advances, 54, 107871. DOI: https://doi.org/10.1016/j.biotechadv.2021.107871

Orelle, C., Carlson, S., Kaushal, B., Almutairi, M. M., Liu, H., Ochabowicz, A., Quan, S., Pham, V. C., Squires, C. L., Murphy, B. T., and Mankin, A. S., 2013. Tools for characterizing bacterial protein synthesis inhibitors. Antimicrobial agents and chemotherapy, 57(12), 5994–6004. DOI: https://doi.org/10.1128/AAC.01673-13

Ngo , T. D. N., Phan , T. H. T., Dinh, T. T., Yurchenko, A. N., Huynh , H. N. K., Le , D. H., Vo , T. D. T., Le, T. H., and Pham, D. T., 2023. Screening of antibacterial and antioxidant activities of marine fungi isolated from the North Sea of Vietnam. Vietnam Journal of Marine Science and Technology, 23(2), 189–201. DOI: https://doi.org/10.15625/1859-3097/17458

Ostrosky, E. A., Mizumoto, M. K., Lima, M. E., Kaneko, T. M., Nishikawa, S. O., and Freitas, B. R., 2008. Métodos para avaliação da atividade antimicrobiana e determinação da concentração mínima inibitória (CMI) de plantas medicinais. Revista brasileira de Farmacognosia, 18, 301–307. DOI: https://doi.org/10.1590/S0102-695X2008000200026

Durães, F., Szemerédi, N., Kumla, D., Pinto, M., Kijjoa, A., Spengler, G., and Sousa, E., 2021. Metabolites from marine-derived fungi as potential antimicrobial adjuvants. Marine drugs, 19(9), 475. DOI: https://doi.org/10.3390/md19090475

Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. DOI: https://doi.org/10.1093/molbev/msy096

Zhou, J., Feng, Z., Zhang, W., and Xu, J., 2022. Evaluation of the antimicrobial and cytotoxic potential of endophytic fungi extracts from mangrove plants Rhizophora stylosa and R. mucronata. Scientific Reports, 12(1), 2733. DOI: https://doi.org/10.1038/s41598-022-06711-9

Fukuda, T., Kurihara, Y., Kanamoto, A., and Tomoda, H., 2014. Terretonin G, a new sesterterpenoid antibiotic from marine-derived Aspergillus sp. OPMF00272. The Journal of Antibiotics, 67(8), 593–595. DOI: https://doi.org/10.1038/ja.2014.46

Song, F., Ren, B., Chen, C., Yu, K., Liu, X., Zhang, Y., Yang, N., He, H., Liu, X., Dai, H., and Zhang, L., 2014. Three new sterigmatocystin analogues from marine-derived fungus Aspergillus versicolor MF359. Applied Microbiology and Biotechnology, 98, 3753–3758. DOI: https://doi.org/10.1007/s00253-013-5409-5

Ding, L., Li, T., Liao, X., He, S., and Xu, S., 2018. Asperitaconic acids A–C, antibacterial itaconic acid derivatives produced by a marine-derived fungus of the genus Aspergillus. The Journal of Antibiotics, 71(10), 902–904. DOI: https://doi.org/10.1038/s41429-018-0079-2

Zhuang, Y., Teng, X., Wang, Y., Liu, P., Wang, H., Li, J., Li, G., and Zhu, W., 2011. Cyclopeptides and polyketides from coral-associated fungus, Aspergillus versicolor LCJ-5-4. Tetrahedron, 67(37), 7085–7089. DOI: https://doi.org/10.1016/j.tet.2011.07.003

Wu, B., Ohlendorf, B., Oesker, V., Wiese, J., Malien, S., Schmaljohann, R., and Imhoff, J. F., 2015. Acetylcholinesterase inhibitors from a marine fungus Talaromyces sp. strain LF458. Marine biotechnology, 17, 110–119. DOI: https://doi.org/10.1007/s10126-014-9599-3

Liu, F., Cai, X. L., Yang, H., Xia, X. K., Guo, Z. Y., Yuan, J., Li, M. F., She, Z. G., and Lin, Y. C., 2010. The bioactive metabolites of the mangrove endophytic fungus Talaromyces sp. ZH-154 isolated from Kandelia candel (L.) Druce. Planta Medica, 76(02), 185–189. DOI: https://doi.org/10.1055/s-0029-1186047