Screening of actinomycetes from Ninh Thuan and Binh Thuan sea for antimicrobial producers

Thi Thu Huyen Vu; Thi Hong Minh Le; Mai Anh Nguyen; Thi Quyen Vu; Thi Mai Huong Doan; Van Cuong Pham

doi:10.15625/1859-3097/17208

Author affiliations

Authors

Vu Thi Thu Huyen Institute of Marine Biochemistry, VAST, Vietnam
Le Thi Hong Minh Institute of Marine Biochemistry, VAST, Vietnam
Nguyen Mai Anh Institute of Marine Biochemistry, VAST, Vietnam
Vu Thi Quyen Institute of Marine Biochemistry, VAST, Vietnam
Doan Thi Mai Huong Institute of Marine Biochemistry, VAST, Vietnam
Pham Van Cuong Institute of Marine Biochemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/17208

Keywords:

Actinomycetes, antimicrobial activity, bioassay, MIC, marine microbial, 16S rRNA.

Abstract

The sea is a unique ecosystem for finding secondary metabolites from marine microorganisms. Many compounds produced by marine microorganisms with antibacterial activity have been applied in life. Furthermore, microorganisms are renewable raw materials and media for drug production, thus crucial for sustainable development in our life. In this study, we screened for antibacterial activity of microbial strains isolated from tern samples from Ninh Thuan and Binh Thuan seas. The antibacterial activity of the marine microbial crude extracts was performed by the Bioassay method in a 96-well tray. The minimum inhibitory concentration (MIC) test results showed that from 86 strains of microorganisms isolated from the waters of Ninh Thuan and Binh Thuan, 68 strains were able to inhibit the growth of 1 to 6 strains of the tested microorganisms, and 27 over 86 strains had antibacterial activity against at least three strains of microorganisms tested. In which strains G631, G756, G769, and G778 inhibited 4 to 6 tested microorganisms with MIC values equal to or lower than positive controls. Morphological characteristics and molecular biological analysis identified the four most active strains. The results showed that all four strains were actinomycetes.

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