Screening of actinomycetes from Ninh Thuan and Binh Thuan sea for antimicrobial producers

Vu Thi Thu Huyen, Le Thi Hong Minh, Nguyen Mai Anh, Vu Thi Quyen, Doan Thi Mai Huong, Pham Van Cuong
Author affiliations


  • Vu Thi Thu Huyen Institute of Marine Biochemistry, VAST, Vietnam
  • Le Thi Hong Minh Institute of Marine Biochemistry, VAST, Vietnam
  • Nguyen Mai Anh Institute of Marine Biochemistry, VAST, Vietnam
  • Vu Thi Quyen Institute of Marine Biochemistry, VAST, Vietnam
  • Doan Thi Mai Huong Institute of Marine Biochemistry, VAST, Vietnam
  • Pham Van Cuong Institute of Marine Biochemistry, VAST, Vietnam



Actinomycetes, antimicrobial activity, bioassay, MIC, marine microbial, 16S rRNA.


The sea is a unique ecosystem for finding secondary metabolites from marine microorganisms. Many compounds produced by marine microorganisms with antibacterial activity have been applied in life. Furthermore, microorganisms are renewable raw materials and media for drug production, thus crucial for sustainable development in our life. In this study, we screened for antibacterial activity of microbial strains isolated from tern samples from Ninh Thuan and Binh Thuan seas. The antibacterial activity of the marine microbial crude extracts was performed by the Bioassay method in a 96-well tray. The minimum inhibitory concentration (MIC) test results showed that from 86 strains of microorganisms isolated from the waters of Ninh Thuan and Binh Thuan, 68 strains were able to inhibit the growth of 1 to 6 strains of the tested microorganisms, and 27 over 86 strains had antibacterial activity against at least three strains of microorganisms tested. In which strains G631, G756, G769, and G778 inhibited 4 to 6 tested microorganisms with MIC values equal to or lower than positive controls. Morphological characteristics and molecular biological analysis identified the four most active strains. The results showed that all four strains were actinomycetes.


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Zhang, L., An, R., Wang, J., Sun, N., Zhang, S., Hu, J., and Kuai, J., 2005. Exploring novel bioactive compounds from marine microbes. Current opinion in microbiology, 8(3), 276–281.

Ng, T. B., Cheung, R. C. F., Wong, J. H., Bekhit, A. A., and Bekhit, A. E. D., 2015. Antibacterial products of marine organisms. Applied Microbiology and Biotechnology, 99, 4145–4173.

Baharum, S. N., Beng, E. K., and Mokhtar, M. A. A., 2010. Marine microorganisms: potential application and challenges. Journal of Biological Sciences, 10(6), 555–564.

Bhatnagar, I., and Kim, S. K., 2010. Immense essence of excellence: marine microbial bioactive compounds. Marine drugs, 8(10), 2673–2701.

Williams, S. T., and Cross, T., 1971. Chapter XI actinomycetes. In Methods in microbiology (Vol. 4, pp. 295–334). Academic press.

Orelle, C., Carlson, S., Kaushal, B., Almutairi, M. M., Liu, H., Ochabowicz, A., Quan, S., Pham, V. C., Squires, C. L., Murphy, B. T., and Mankin, A. S., 2013. Tools for characterizing bacterial protein synthesis inhibitors. Antimicrobial agents and chemotherapy, 57(12), 5994–6004.

Hadacek, F., and Greger, H., 2000. Testing of antifungal natural products: methodologies, comparability of results and assay choice. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 11(3), 137–147.

Muthu, M. R., Subbaiya, R., Balasubramanian, M., Ponmurugan, P., and Selvam, M., 2013. Isolation and identification of actinomycetes Isoptericola variabilis from Cauvery river soil sample. Int. J. Curr. Microbiol. App. Sci, 2(6), 236–245.

Kim, M., and Chun, J., 2014. 16S rRNA gene-based identification of bacteria and archaea using the EzTaxon server. In Methods in microbiology (Vol. 41, pp. 61–74). Academic Press.

Schlecht, L. M., Peters, B. M., Krom, B. P., Freiberg, J. A., Hänsch, G. M., Filler, S. G., Jabra-Rizk, M. A., and Shirtliff, M. E., 2015. Systemic Staphylococcus aureus infection mediated by Candida albicans hyphal invasion of mucosal tissue. Microbiology, 161(Pt 1), 168–181.

Saitou, N., and Nei, M., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular biology and evolution, 4(4), 406–425.

Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K., 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6), 1547–1549.

Galaviz-Silva, L., Iracheta-Villarreal, J. M., and Molina-Garza, Z. J., 2018. Bacillus and Virgibacillus strains isolated from three Mexican coasts antagonize Staphylococcus aureus and Vibrio parahaemolyticus. FEMS microbiology letters, 365(19), fny202.

Shin, D., Byun, W. S., Moon, K., Kwon, Y., Bae, M., Um, S., Lee, S. K., and Oh, D. C., 2018. Coculture of marine Streptomyces sp. with Bacillus sp. produces a new piperazic acid-bearing cyclic peptide. Frontiers in chemistry, 6, 498.

Hifnawy, M. S., Fouda, M. M., Sayed, A. M., Mohammed, R., Hassan, H. M., AbouZid, S. F., Rated, M. E., Keller, A., Adamek, M., Ziemert, N., and Abdelmohsen, U. R., 2020. The genus Micromonospora as a model microorganism for bioactive natural product discovery. RSC advances, 10(35), 20939–20959.

Pérez-Bonilla, M., Oves-Costales, D., Cruz, M. D. L., Kokkini, M., Martín, J., Vicente, F., Genilloud, O., and Reyes, F., 2018. Phocoenamicins B and C, new antibacterial spirotetronates isolated from a marine Micromonospora sp. Marine drugs, 16(3), 95.

Qi, S., Gui, M., Li, H., Yu, C., Li, H., Zeng, Z., and Sun, P., 2020. Secondary metabolites from marine micromonospora: Chemistry and bioactivities. Chemistry & Biodiversity, 17(4), e2000024.

Urzì, C., Salamone, P., Schumann, P., Rohde, M., and Stackebrandt, E., 2004. Blastococcus saxobsidens sp. nov., and emended descriptions of the genus Blastococcus Ahrens and Moll 1970 and Blastococcus aggregatus Ahrens and Moll 1970. International journal of systematic and evolutionary microbiology, 54(1), 253–259.

Yan, Z. F., Lin, P., Chu, X., Kook, M., Li, C. T., and Yi, T. H., 2016. Aeromicrobium halotolerans sp. nov., isolated from desert soil sample. Archives of Microbiology, 198, 423–427.

Yang, Z. W., Asem, M. D., Li, X., Li, L. Y., Salam, N., Alkhalifah, D. H. M., Hozzein, W. N., Nie, G. X., and Li, W. J., 2019. Blastococcus deserti sp. nov., isolated from a desert sample. Archives of microbiology, 201, 193–198.

Normand, P., Daffonchio, D., and Gtari, M., 2014. The family geodermatophilaceae. In The prokaryotes: actinobacteria (pp. 361–379). Springer-Verlag Berlin Heidelberg.

Contreras-Castro, L., Martinez-Garcia, S., Cancino-Diaz, J. C., Maldonado, L. A., Hernandez-Guerrero, C. J., Martínez-Díaz, S. F., González-Acosta, B., and Quintana, E. T., 2020. Marine Sediment Recovered sp. Inhibits the Growth of Emerging Bacterial Pathogens and other Multi-Drug-Resistant Bacteria. Polish Journal of Microbiology, 69(3), 321–330.

Bose, U., Hewavitharana, A. K., Vidgen, M. E., Ng, Y. K., Shaw, P. N., Fuerst, J. A., and Hodson, M. P., 2014. Discovering the recondite secondary metabolome spectrum of Salinispora species: a study of inter-species diversity. PloS one, 9(3), e91488.

Nouioui, I., Rückert, C., Willemse, J., van Wezel, G. P., Klenk, H. P., Busche, T., Kalinowski, J., Bredholt, H., and Zotchev, S. B., 2017. Actinoalloteichus fjordicus sp. nov. isolated from marine sponges: phenotypic, chemotaxonomic and genomic characterisation. Antonie van Leeuwenhoek, 110, 1705–1717.

Fujita, K., Sugiyama, R., Nishimura, S., Ishikawa, N., Arai, M. A., Ishibashi, M., and Kakeya, H., 2016. Stereochemical assignment and biological evaluation of BE-14106 unveils the importance of one acetate unit for the antifungal activity of polyene macrolactams. Journal of Natural Products, 79(7), 1877–1880.

Wang, X. J., Zhang, J., Wang, J. D., Qian, P. T., Liu, C. X., and Xiang, W. S., 2013. Novel cyclopentenone derivatives produced by a rare actinobacterial strain Actinoalloteichus nanshanensis sp. nov. NEAU 119. Natural product research, 27(20), 1863–1869.

Sato, S., Iwata, F., Yamada, S., and Katayama, M., 2012. Neomaclafungins A–I: oligomycin-class macrolides from a marine-derived actinomycete. Journal of Natural Products, 75(11), 1974–1982.

Fu, P., Kong, F., Li, X., Wang, Y., and Zhu, W., 2014. Cyanogramide with a new spiro [indolinone-pyrroloimidazole] skeleton from Actinoalloteichus cyanogriseus. Organic letters, 16(14), 3708–3711.

Fu, P., Wang, S., Hong, K., Li, X., Liu, P., Wang, Y., and Zhu, W., 2011. Cytotoxic bipyridines from the marine-derived actinomycete Actinoalloteichus cyanogriseus WH1-2216-6. Journal of natural products, 74(8), 1751–1756.

Zhang, X., Song, C., Bai, Y., Hu, J., and Pan, H., 2021. Cytotoxic and antimicrobial activities of secondary metabolites isolated from the deep-sea-derived Actinoalloteichus cyanogriseus 12A22. 3 Biotech, 11(6), 283.




How to Cite

Vu, T. T. H., Le, T. H. M., Nguyen, M. A., Vu, T. Q., Doan, T. M. H., & Pham, V. C. (2022). Screening of actinomycetes from Ninh Thuan and Binh Thuan sea for antimicrobial producers. Vietnam Journal of Marine Science and Technology, 22(4), 423–432.




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